LOCUS       Exported                2605 bp ds-DNA    circular SYN 02-OCT-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pUO-pp-303-2-pAOX1
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ulli
  TITLE     Direct Submission
  JOURNAL   Exported 24 Oct 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label="BsmBI"
                     /note="BsmBI"
                     /note="color: #00ff15"
     misc_feature    complement(7..115)
                     /label="CamR Terminator"
                     /note="CamR Terminator"
                     /note="color: #66ccff; direction: LEFT"
     CDS             complement(116..775)
                     /label="CamR"
                     /note="CamR"
                     /note="color: #0000ff; direction: LEFT"
     promoter        complement(776..880)
                     /label="CamR Promoter"
                     /note="CamR Promoter"
                     /note="color: #66ccff; direction: LEFT"
     rep_origin      complement(881..1644)
                     /direction=LEFT
                     /label="ColE1"
                     /note="ColE1"
                     /note="color: #7f7f7f"
     promoter        1660..2598
                     /gene="Pichia pastoris AOX1"
                     /note="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
       61 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      181 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      241 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      841 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
      901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtagtcgg tctcaaacgg atctaacatc caaagacgaa
     1681 aggttgaatg aaaccttttt gccatccgac atccacaggt ccattctcac acataagtgc
     1741 caaacgcaac aggaggggat acactagcag cagaccgttg caaacgcagg acctccactc
     1801 ctcttctcct caacacccac ttttgccatc gaaaaaccag cccagttatt gggcttgatt
     1861 ggagctcgct cattccaatt ccttctatta ggctactaac accatgactt tattagcctg
     1921 tctatcctgg cccccctggc gaggttcatg tttgtttatt tccgaatgca acaagctccg
     1981 cattacaccc gaacatcact ccagatgagg gctttctgag tgtggggtca aatagtttca
     2041 tgttccccaa atggcccaaa actgacagtt taaacgctgt cttggaacct aatatgacaa
     2101 aagcgtgatc tcatccaaga tgaactaagt ttggttcgtt gaaatgctaa cggccagttg
     2161 gtcaaaaaga aacttccaaa agtcggcata ccgtttgtct tgtttggtat tgattgacga
     2221 atgctcaaaa ataatctcat taatgcttag cgcagtctct ctatcgcttc tgaaccccgg
     2281 tgcacctgtg ccgaaacgca aatggggaaa cacccgcttt ttggatgatt atgcattgtc
     2341 tccacattgt atgcttccaa gattctggtg ggaatactgc tgatagccta acgttcatga
     2401 tcaaaattta actgttctaa cccctacttg acagcaatat ataaacagaa ggaagctgcc
     2461 ctgtcttaaa cctttttttt tatcatcatt attagcttac tttcataatt gcgactggtt
     2521 ccaattgaca agcttttgat tttaacgact tttaacgaca acttgagaag atcaaaaaac
     2581 aactaattat tcgaaacgta tgtga
//
