LOCUS       Exported                2664 bp ds-DNA     circular SYN 10-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPTK003
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ulli
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 10, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label=BsmBI
                     /note="BsmBI"
     misc_feature    complement(7..115)
                     /label=CamR Terminator
                     /note="CamR Terminator"
     CDS             complement(116..775)
                     /label=CamR
                     /note="CamR"
     promoter        complement(776..880)
                     /label=CamR Promoter
                     /note="CamR Promoter"
     rep_origin      complement(881..1644)
                     /direction=LEFT
                     /label=ColE1
                     /note="ColE1"
     misc_feature    1660..2657
                     /note="pENO"
     misc_feature    2349..2354
                     /note="OLD BsmBI"
     misc_feature    2350
                     /note="g to c to remove BsmBI"
     misc_feature    2423..2428
                     /note="OLD BsaI site"
     misc_feature    2424
                     /note="g to c to remove BsaI"
ORIGIN
        1 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
       61 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      181 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      241 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      841 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
      901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtagtcgg tctcaaacga gaaagcatac tatactattc
     1681 gacattcctt tcaatcctgg aattaacagt cacttttaaa aaagacatct accgtgaagg
     1741 tgccgtagag tatcgcgtta ccatatcgcc aaaaactgat atacgccgcg gaaaccaggc
     1801 aaacaattga aaagaaaaat tttgaggaac tctctgcatc gaagccgtct agagttacca
     1861 ctagtcagat gccgcgggca cttgagcacc tcatgcacag caataacaca acacaatggt
     1921 tagtagcaac ctgaattcgg tcattgatgc atgcatgtgc cgtgaagcgg gacaaccaga
     1981 aaagtcgtct ataaatgccg gcacgtgcga tcatcgtggc ggggttttaa gagtgcatat
     2041 cacaaattgt cgcattaccg cggaaccgcc agatattcat tacttgacgc aaaagcgttt
     2101 gaaataatga cgaaaaagaa ggaagaaaaa aaaagaaaaa taccgcttct aggcgggtta
     2161 tctactgatc cgagcttcca ctaggatagc acccaaacac ctgcatattt ggacgacctt
     2221 tacttacacc accaaaaacc actttcgcct ctcccgcccc tgataacgtc cactaattga
     2281 gcgattacct gagcggtcct cttttgtttg cagcatgaga cttgcatact gcaaatcgta
     2341 agtagcaacc tctcaaggtc aaaactgtat ggaaaccttg tcacctcact taattctagc
     2401 tagcctaccc tgcaagtcaa gagctctccg tgattcctag ccacctcaag gtatgcctct
     2461 ccccggaaac tgtggccttt tctggcacac atgatctcca cgatttcaac atataaatag
     2521 cttttgataa tggcaatatt aatcaaattt attttacttc tttcttgtaa catctctctt
     2581 gtaatccctt attccttcta gctatttttc ataaaaaacc aagcaactgc ttatcaacac
     2641 acaaacacta aatcaaatat gtga
//
