LOCUS       Exported                2403 bp ds-DNA    circular SYN 30-OCT-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pUO-pp-327-3b-RFP-Linker
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ulli
  TITLE     Direct Submission
  JOURNAL   Exported 24 Oct 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label="BsmBI"
                     /note="BsmBI"
                     /note="color: #00ff15"
     misc_feature    complement(7..115)
                     /label="CamR Terminator"
                     /note="CamR Terminator"
                     /note="color: #66ccff; direction: LEFT"
     CDS             complement(116..775)
                     /label="CamR"
                     /note="CamR"
                     /note="color: #0000ff; direction: LEFT"
     promoter        complement(776..880)
                     /label="CamR Promoter"
                     /note="CamR Promoter"
                     /note="color: #66ccff; direction: LEFT"
     rep_origin      complement(881..1644)
                     /direction=LEFT
                     /label="ColE1"
                     /note="ColE1"
                     /note="color: #7f7f7f"
     misc_feature    1660..2394
                     /note="RFP"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1660..1662
                     /note="Start with second codon!!! ATG deleted!"
                     /note="color: #ff00ff"
     misc_feature    1674..1679
                     /note="OLD BsaI"
                     /note="color: #00ff00"
     misc_feature    1677
                     /note="c to g to remove BsaI"
                     /note="color: #ff0000"
     misc_feature    1791..1796
                     /note="OLD BsaI"
                     /note="color: #00ff00"
     misc_feature    1794
                     /note="a to g to remove BsaI"
                     /note="color: #ff0000"
ORIGIN
        1 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
       61 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      181 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      241 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      841 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
      901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtagtcgg tctcattctg caacttccgg tatggtgtca
     1681 aagggagagg aaaataatat ggctattatt aaggagttta tgcgttttaa ggtacatatg
     1741 gaaggttctg tcaacggtca cgaattcgaa attgaaggtg agggggaggg gaggccatac
     1801 gagggaactc agactgctaa gttaaaggtc actaaaggtg gtcctttacc tttcgcctgg
     1861 gatatcctgt ctccacagtt tatgtacggt tcaaaggctt atgtgaaaca tcctgccgat
     1921 atcccagatt atcttaaact ttctttccct gagggtttta agtgggagag ggtaatgaac
     1981 tttgaagacg gtggtgtggt cactgttact caggactcaa gtctgcagga cggtgagttc
     2041 atctacaagg tgaagctgag aggtaccaat tttccatcag atggtcccgt gatgcaaaaa
     2101 aagacaatgg gttgggaagc ttctagtgaa cgtatgtatc ccgaagatgg agctttgaaa
     2161 ggtgaaatta agcaaagact aaaacttaag gatggtggac attacgatgc tgaagttaag
     2221 acgacctaca aggccaaaaa gccagtccag ttgcctggag catacaatgt taacatcaaa
     2281 ttggatataa cttcccataa tgaagactat accatcgtcg agcaatacga acgagccgaa
     2341 gggagacaca gtactggtgg tatggatgaa ctttataaag gatccggaac cgcaggatcc
     2401 tga
//
