LOCUS       pBbA5c-Mev             10432 bp    DNA     circular
ACCESSION   pBbA5c-MevTaco-MKco-PMKco
VERSION     pBbA5c-MevTaco-MKco-PMKco.1
KEYWORDS    .
FEATURES             Location/Qualifiers
     rep_origin      complement(8724..9435)
                     /label=p15a
                     /vntifkey="33"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="pink"
     misc_binding    1516..1579
                     /label=lac operator
                     /vntifkey="20"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="pink"
     terminator      9526..9631
                     /label=TO
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="pink"
                     /vntifkey="43"
                     /ApEinfo_fwdcolor="#e70123"
     CDS             complement(49..1131)
                     /label=lacI
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#aae353"
                     /vntifkey="4"
                     /note="lacI does not contain internal EcoRV site"
     misc_RNA        5831..5833
                     /label=null
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8589..8717
                     /label=dbl term
                     /vntifkey="21"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#4efc03"
                     /ApEinfo_revcolor="#0080ff"
     misc_marker     complement(9647..10306)
                     /label=CmR
                     /ApEinfo_fwdcolor="pink"
                     /vntifkey="22"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1500..1575
                     /label=lacUV5 promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="30"
                     /ApEinfo_revcolor="pink"
     CDS             4310..5818
                     /label=HMGR
                     /ApEinfo_fwdcolor="#aae353"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /gene="HMGR"
                     /SECDrawAs="Gene"
                     /vntifkey="4"
     CDS             1610..2791
                     /label=AtoB
                     /ApEinfo_fwdcolor="#aae353"
                     /vntifkey="4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="pink"
     CDS             2815..4290
                     /label=HMGS
                     /vntifkey="4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#aae353"
                     /SECDrawAs="Gene"
                     /gene="HMGS"
                     /ApEinfo_revcolor="pink"
     CDS             7206..8561
                     /label=PMK-fixed
                     /vntifkey="21"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5854..7185
                     /label=MK
                     /ApEinfo_fwdcolor="#00ffff"
                     /vntifkey="4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="#00ffff"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa tgaaaaattg
     1621 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
     1681 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
     1741 ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc tggggcaaaa
     1801 tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
     1861 caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg ccattcaggc
     1921 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag ccccctactt
     1981 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
     2041 cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
     2101 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
     2161 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
     2221 tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga aagcgaactc
     2281 aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
     2341 tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
     2401 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
     2461 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
     2521 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
     2581 ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
     2641 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
     2701 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg gcggcggtca
     2761 gggaattgcg atggtgattg aacggttgaa ttgaggatct aggaggaaag caaaatgaaa
     2821 ctgagcacca agctgtgctg gtgtggcatc aagggtcgcc tgcgcccaca aaagcagcaa
     2881 cagctgcaca acacgaacct gcaaatgacc gagctgaaaa agcagaagac ggccgagcaa
     2941 aagacccgcc cgcagaacgt tggcatcaag ggcatccaga tttatatccc gacgcagtgt
     3001 gtcaaccaat ctgagctgga gaaattcgat ggcgtcagcc agggtaagta caccatcggc
     3061 ctgggccaga ccaacatgag cttcgtgaac gaccgtgagg acatctattc tatgagcctg
     3121 acggtgctgt ctaagctgat caagagctac aacatcgaca cgaataagat cggtcgtctg
     3181 gaggtgggta cggagacgct gattgacaag agcaaaagcg tgaagtctgt cttaatgcag
     3241 ctgttcggcg agaacacgga tgtcgagggt atcgacaccc tgaacgcgtg ttacggcggc
     3301 accaacgcac tgttcaatag cctgaactgg attgagagca acgcctggga tggccgcgat
     3361 gcgatcgtcg tgtgcggcga tatcgccatc tatgacaagg gtgcggcacg tccgaccggc
     3421 ggtgcaggca ccgttgcgat gtggattggc ccggacgcac caattgtctt cgattctgtc
     3481 cgcgcgtctt acatggagca cgcctacgac ttttacaagc cggacttcac gagcgaatac
     3541 ccgtacgtgg acggccactt ctctctgacc tgctatgtga aggcgctgga ccaggtttat
     3601 aagtcttata gcaaaaaggc gatttctaag ggcctggtca gcgacccggc aggcagcgac
     3661 gccctgaacg tgctgaagta tttcgactac aacgtgttcc atgtcccgac ctgcaaatta
     3721 gtgaccaaat cttatggccg cctgttatat aatgatttcc gtgccaaccc gcagctgttc
     3781 ccggaggttg acgccgagct ggcgacgcgt gattacgacg agagcctgac cgacaagaac
     3841 atcgagaaga ccttcgtcaa cgtcgcgaag ccgttccaca aagagcgtgt ggcccaaagc
     3901 ctgatcgtcc cgaccaacac gggcaacatg tataccgcgt ctgtctacgc ggcattcgcg
     3961 agcctgctga attacgtcgg ttctgacgac ctgcagggca agcgcgttgg cctgttcagc
     4021 tacggtagcg gcttagcggc cagcctgtat agctgcaaaa ttgtcggcga cgtccagcac
     4081 atcatcaagg agctggacat caccaacaag ctggcgaagc gcatcaccga gacgccgaaa
     4141 gattacgagg cagcgatcga gttacgcgag aatgcgcatc tgaagaagaa cttcaagccg
     4201 caaggtagca tcgagcacct gcagagcggc gtctactacc tgacgaacat tgacgacaag
     4261 ttccgccgtt cttatgacgt caaaaagtaa ctagtaggag gaaaacatca tggtgctgac
     4321 gaacaaaacc gtcattagcg gcagcaaggt gaagtctctg agcagcgccc aaagctctag
     4381 cagcggcccg tctagcagca gcgaggagga cgacagccgt gacattgagt ctctggacaa
     4441 gaagatccgc ccgctggagg agttagaggc cctgctgagc agcggcaaca ccaagcagct
     4501 gaagaacaag gaagttgcag cgctggtgat ccacggtaag ctgccactgt atgcgctgga
     4561 aaagaaactg ggcgatacga cgcgtgcggt cgcggtgcgt cgcaaagcct taagcatctt
     4621 agcggaggcc ccggtgttag ccagcgaccg cctgccgtac aagaactacg actacgaccg
     4681 cgtgtttggc gcgtgctgcg agaatgtcat tggctacatg ccgttaccgg ttggtgtgat
     4741 cggcccgctg gtcattgatg gcacgagcta tcacattcca atggcgacca cggaaggttg
     4801 cttagtcgcc agcgccatgc gtggctgtaa ggcgattaac gccggcggtg gcgcgacgac
     4861 cgtgttaacc aaggatggta tgacgcgcgg tccggtcgtc cgcttcccaa cgctgaagcg
     4921 cagcggcgcg tgtaagattt ggctggattc tgaggagggc caaaacgcga tcaagaaagc
     4981 cttcaactct acgagccgtt tcgcgcgttt acagcatatc cagacctgcc tggccggcga
     5041 cctgctgttc atgcgcttcc gcaccaccac gggcgatgcg atgggcatga acatgatcag
     5101 caagggcgtc gaatatagcc tgaaacaaat ggtggaagaa tatggctggg aggacatgga
     5161 ggttgtctct gtgagcggca actattgcac cgacaagaag ccggcagcca ttaactggat
     5221 tgagggtcgc ggcaaaagcg tcgtggcaga agcgaccatc ccaggcgacg tggtccgtaa
     5281 ggttctgaag agcgacgtca gcgccctggt tgagttaaat atcgcgaaaa acctggtcgg
     5341 cagcgcgatg gcgggcagcg tgggtggctt taacgcacat gcagcgaatc tggttacggc
     5401 ggttttctta gccttaggtc aggacccagc ccaaaatgtc gagagcagca actgcattac
     5461 cttaatgaaa gaggttgacg gtgacctgcg catcagcgtt tctatgccgt ctatcgaggt
     5521 cggcacgatc ggcggcggca ccgttttaga accgcaaggt gcgatgctgg atctgctggg
     5581 cgtgcgcggc ccacatgcaa cggccccagg caccaatgcc cgccaactgg cccgtatcgt
     5641 ggcctgcgcg gttctggcgg gtgagctgag cctgtgcgcc gcattagccg cgggccattt
     5701 agttcaatct cacatgaccc acaaccgcaa gccggcagaa ccaaccaagc caaataacct
     5761 ggacgcaacc gacattaacc gtctgaagga tggcagcgtc acgtgcatta aaagctgagg
     5821 atctggatct aaaggatcta ggaggaaata accatgtctc tgccattcct gacgtctgcg
     5881 ccaggtaagg tgatcatctt cggcgagcac tctgcggtgt acaataagcc ggccgtcgcc
     5941 gcctctgtgt ctgcgttacg cacctacctg ctgatcagcg aatcttctgc accggacacg
     6001 atcgagctgg actttccgga catcagcttc aaccacaagt ggagcatcaa cgacttcaac
     6061 gcgatcacgg aggaccaggt gaacagccaa aagctggcca aagcccagca agcaaccgac
     6121 ggtctgtctc aggagctggt gtctctgctg gacccgctgt tagcgcagtt aagcgagagc
     6181 ttccattacc acgccgcgtt ctgcttcctg tacatgttcg tttgcctgtg cccgcacgca
     6241 aagaacatca agttcagcct gaagagcacg ctgccgattg gcgcaggctt aggctctagc
     6301 gcatctatca gcgtgagcct ggcgctggcg atggcctatc tgggtggcct gattggcagc
     6361 aacgacctgg agaaactgag cgaaaacgac aagcacatcg tgaaccagtg ggcctttatc
     6421 ggcgagaagt gcattcatgg caccccgagc ggcattgaca acgcagttgc cacgtatggc
     6481 aacgccctgc tgttcgagaa agacagccac aacggcacga tcaacacgaa caacttcaag
     6541 ttcctggacg acttcccggc gatcccgatg attctgacct acacccgtat cccacgcagc
     6601 accaaggatt tagtcgcccg cgtgcgtgtt ttagtcaccg aaaagttccc ggaggtgatg
     6661 aagccgatcc tggacgcgat gggcgagtgc gcgctgcagg gtctggagat catgaccaag
     6721 ctgagcaagt gcaagggcac cgacgatgag gcggtggaaa ccaacaatga gctgtacgag
     6781 cagctgctgg agctgatccg tatcaatcac ggcctgctgg tttctatcgg tgtgtctcac
     6841 ccgggcctgg aactgatcaa aaacctgagc gacgacctgc gcattggctc tacgaaatta
     6901 acgggtgcag gtggcggtgg ctgctcttta acgctgctgc gccgtgacat tacgcaggag
     6961 caaatcgaca gcttcaagaa gaagctgcag gacgacttca gctacgagac gttcgagacg
     7021 gacctgggcg gcacgggctg ttgcctgctg agcgccaaaa atctgaacaa ggacctgaag
     7081 atcaaaagcc tggtgttcca gctgttcgaa aacaagacga ccacgaagca gcagatcgac
     7141 gacctgttac tgccgggtaa caccaatctg ccgtggacgt cttaaggatc taggagggag
     7201 atcatatgag cgaattacgt gcattcagcg cgccaggtaa ggcactgctg gccggtggct
     7261 acctggtgtt agacaccaag tacgaggcgt tcgtcgtcgg cttatctgcc cgtatgcatg
     7321 cagttgccca cccgtatggt agcctgcagg gctctgacaa gttcgaagtg cgtgtgaaga
     7381 gcaagcagtt caaggacggc gagtggctgt accacattag cccaaagagc ggcttcatcc
     7441 cggttagcat tggtggcagc aagaacccat ttatcgagaa ggtcattgcc aacgtcttca
     7501 gctacttcaa gccgaatatg gacgattact gcaaccgcaa cctgttcgtc atcgacattt
     7561 tcagcgacga cgcgtaccac agccaagagg actctgttac ggagcatcgt ggtaaccgcc
     7621 gcctgagctt ccacagccat cgcattgagg aggtgccgaa gacgggtctg ggttctagcg
     7681 ccggtttagt taccgtctta acgacggcgt tagcgagctt cttcgtgagc gacctggaga
     7741 acaacgtgga caagtaccgc gaagtgattc ataacctggc gcaggtggca cattgtcagg
     7801 cccaaggtaa gattggctct ggttttgatg tggcagcggc cgcctatggc tctatccgct
     7861 atcgccgctt tccgccggcc ctgatcagca atctgccgga catcggctct gcgacgtatg
     7921 gtagcaaact ggcgcatctg gtggacgaag aagactggaa catcaccatt aagtctaatc
     7981 acctgccgag cggcttaacg ttatggatgg gcgatatcaa gaacggcagc gaaacggtta
     8041 agctggtgca gaaagtgaaa aactggtacg acagccacat gccggaaagc ctgaagattt
     8101 acacggagct ggaccacgcc aatagccgtt tcatggatgg tctgagcaag ctggaccgcc
     8161 tgcacgaaac ccacgacgac tacagcgacc aaatcttcga gagcctggag cgcaatgact
     8221 gcacctgcca gaagtacccg gagatcacgg aggtccgcga tgccgtggca acgattcgcc
     8281 gtagcttccg caaaattacg aaggagagcg gcgcggatat cgaaccaccg gtccagacgt
     8341 ctctgctgga cgactgtcaa accttaaagg gcgtgttaac gtgcctgatt ccgggcgcgg
     8401 gtggttacga cgccattgcc gtcatcacga aacaggacgt cgatctgcgc gcacaaacgg
     8461 ccaacgacaa acgtttcagc aaagtccaat ggctggatgt tacgcaggcc gactggggtg
     8521 ttcgcaagga gaaggacccg gaaacgtatc tggataagtg attaggatcc aaactcgagt
     8581 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     8641 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     8701 gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc
     8761 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     8821 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     8881 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     8941 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     9001 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     9061 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     9121 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     9181 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     9241 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     9301 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     9361 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     9421 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
     9481 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
     9541 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     9601 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     9661 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     9721 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     9781 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     9841 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     9901 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     9961 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
    10021 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
    10081 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
    10141 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
    10201 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
    10261 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
    10321 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
    10381 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata tc
//
