LOCUS       P882_AMPH-BAR-mC        6292 bp    DNA     circular     19-APR-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|623771805|
COMMENT     VNTDBDATE|623771805|
COMMENT     LSOWNER|
COMMENT     VNTNAME|P882_AMPH-BAR-mCherry|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    18..381
                     /vntifkey="21"
                     /label=CMV\enhancer
     intron          662..1625
                     /vntifkey="15"
                     /label=Chicken\beta-actin\intron
     promoter        382..661
                     /vntifkey="29"
                     /label=Chicken\beta-actin\promoter
     intron          1626..1718
                     /vntifkey="15"
                     /label=Rabbit\globin\intron
     polyA_signal    3239..3773
                     /vntifkey="25"
                     /label=Rabbit\globin\polyA
     rep_origin      3964..4300
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      4511..5145
                     /vntifkey="33"
                     /label=ColiE1\ori
     CDS             complement(5302..6162)
                     /vntifkey="4"
                     /label=AmpR
     promoter        18..1718
                     /vntifkey="29"
                     /label=CAG\promoter
     CDS             1725..2492
                     /vntifkey="4"
                     /label=AMPH-BAR
     CDS             2508..3218
                     /vntifkey="4"
                     /label=mCherry
BASE COUNT     1410 a      1671 c      1771 g      1440 t 
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcatggcc gacatcaaga 
     1741 cgggcatctt cgccaagaac gtccagaagc gactcaaccg cgcgcaggaa aaggtcctcc 
     1801 aaaagctggg gaaagctgat gagacaaaag acgaacagtt cgaagaatat gtccagaact 
     1861 tcaaacggca agaagcagag ggtaccagac ttcagcgaga actccgagga tatttagcag 
     1921 caatcaaagg catgcaggag gcctccatga agctcacaga gtcgctgcat gaagtctatg 
     1981 agcctgactg gtatgggcgg gaagatgtga aaatggttgg tgagaaatgt gatgtgctgt 
     2041 gggaagactt ccatcaaaaa ctcgtggatg ggtccttgct aacactggat acctacctgg 
     2101 ggcaatttcc tgacataaag aatcgcatcg ccaagcgcag caggaagcta gtggactatg 
     2161 acagtgcccg ccaccatctg gaagctctgc agagctccaa gaggaaggat gagagtcgaa 
     2221 tctctaaggc agaagaagaa tttcagaaag cacagaaagt gtttgaagag tttaacgttg 
     2281 acttacaaga agagttacca tcattatggt caagacgagt tggattttat gttaatactt 
     2341 tcaaaaacgt ctccagcctt gaagccaagt ttcataagga aattgcggtg ctttgccaca 
     2401 aactgtatga agtgatgaca aaactgggtg accagcacgc cgacaaggcc ttcaccatcc 
     2461 aaggagcgcc cagtgattcg ggtcctctcc gcggaccggt cgccaccatg gtgagcaagg 
     2521 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg 
     2581 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 
     2641 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 
     2701 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 
     2761 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 
     2821 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct 
     2881 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 
     2941 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 
     3001 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca 
     3061 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg 
     3121 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc 
     3181 gccactccac cggcggcatg gacgagctgt acaagtaact cgaggatatc gcggccgcac 
     3241 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca 
     3301 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct 
     3361 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa 
     3421 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa 
     3481 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt 
     3541 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata 
     3601 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt 
     3661 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac 
     3721 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg cagcccaagc 
     3781 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     3841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     3901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     3961 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     4021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     4081 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     4141 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 
     4201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     4261 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 
     4321 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     4381 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     4441 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     4501 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     4561 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     4621 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     4681 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 
     4741 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     4801 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     4861 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     4921 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     4981 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     5041 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     5101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     5161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     5221 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     5281 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     5341 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     5401 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     5461 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     5521 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     5581 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     5641 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     5701 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     5761 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     5821 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     5881 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     5941 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     6001 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     6061 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     6121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     6181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     6241 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg 
//
