LOCUS       P1072_CDH5-mRuby3-IRES-Puro.gb       12398 bp    DNA     circular      3-JUN-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT       (CSeqView 10 10 (CObjectList #285=(XLATFRAGMENT 6330 237 0 1 6331 713)) 
COMMENT        (CObList #286=(AnlzXLATItem 6330 7043 1 6330 7043)) 1 (CObList)) 
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FEATURES             Location/Qualifiers
     misc_feature    8320..8911
                     /vntifkey="21"
                     /label=WPRE
     misc_structure  2028..2151
                     /vntifkey="88"
                     /label=cPPT
     CDS             7697..8296
                     /vntifkey="4"
                     /label=Puro
     promoter        2220..3920
                     /vntifkey="29"
                     /label=CAG
     CDS             3931..6282
                     /vntifkey="4"
                     /label=CDH5
     CDS             6331..7044
                     /vntifkey="4"
                     /label=mRuby3
BASE COUNT     2972 a      3233 c      3467 g      2726 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgacat tgattattga 
     2221 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 
     2281 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 
     2341 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 
     2401 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 
     2461 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 
     2521 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 
     2581 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca 
     2641 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg 
     2701 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag 
     2761 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg 
     2821 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc 
     2881 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc 
     2941 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt 
     3001 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga 
     3061 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga 
     3121 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct 
     3181 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg 
     3241 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg 
     3301 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag 
     3361 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg 
     3421 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag 
     3481 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 
     3541 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 
     3601 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag 
     3661 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc 
     3721 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg 
     3781 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta 
     3841 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc 
     3901 tgtctcatca ttttggcaaa gaattccacc atgcagaggc tcatgatgct cctcgccaca 
     3961 tcgggcgcct gcctgggcct gctggcagtg gcagcagtgg cagcagcagg tgctaaccct 
     4021 gcccaacggg acacccacag cctgctgccc acccaccggc gccaaaagag agattggatt 
     4081 tggaaccaga tgcacattga tgaagagaaa aacacctcac ttccccatca tgtaggcaag 
     4141 atcaagtcaa gcgtgagtcg caagaatgcc aagtacctgc tcaaaggaga atatgtgggc 
     4201 aaggtcttcc gggtcgatgc agagacagga gacgtgttcg ccattgagag gctggaccgg 
     4261 gagaatatct cagagtacca cctcactgct gtcattgtgg acaaggacac tggcgaaaac 
     4321 ctggagactc cttccagctt caccatcaaa gttcatgacg tgaacgacaa ctggcctgtg 
     4381 ttcacgcatc ggttgttcaa tgcgtccgtg cctgagtcgt cggctgtggg gacctcagtc 
     4441 atctctgtga cagcagtgga tgcagacgac cccactgtgg gagaccacgc ctctgtcatg 
     4501 taccaaatcc tgaaggggaa agagtatttt gccatcgata attctggacg tattatcaca 
     4561 ataacgaaaa gcttggaccg agagaagcag gccaggtatg agatcgtggt ggaagcgcga 
     4621 gatgcccagg gcctccgggg ggactcgggc acggccaccg tgctggtcac tctgcaagac 
     4681 atcaatgaca acttcccctt cttcacccag accaagtaca catttgtcgt gcctgaagac 
     4741 acccgtgtgg gcacctctgt gggctctctg tttgttgagg acccagatga gccccagaac 
     4801 cggatgacca agtacagcat cttgcggggc gactaccagg acgctttcac cattgagaca 
     4861 aaccccgccc acaacgaggg catcatcaag cccatgaagc ctctggatta tgaatacatc 
     4921 cagcaataca gcttcatcgt cgaggccaca gaccccacca tcgacctccg atacatgagc 
     4981 cctcccgcgg gaaacagagc ccaggtcatt atcaacatca cagatgtgga cgagcccccc 
     5041 attttccagc agcctttcta ccacttccag ctgaaggaaa accagaagaa gcctctgatt 
     5101 ggcacagtgc tggccatgga ccctgatgcg gctaggcata gcattggata ctccatccgc 
     5161 aggaccagtg acaagggcca gttcttccga gtcacaaaaa agggggacat ttacaatgag 
     5221 aaagaactgg acagagaagt ctacccctgg tataacctga ctgtggaggc caaagaactg 
     5281 gattccactg gaacccccac aggaaaagaa tccattgtgc aagtccacat tgaagttttg 
     5341 gatgagaatg acaatgcccc ggagtttgcc aagccctacc agcccaaagt gtgtgagaac 
     5401 gctgtccatg gccagctggt cctgcagatc tccgcaatag acaaggacat aacaccacga 
     5461 aacgtgaagt tcaaattcac cttgaatact gagaacaact ttaccctcac ggataatcac 
     5521 gataacacgg ccaacatcac agtcaagtat gggcagtttg accgggagca taccaaggtc 
     5581 cacttcctac ccgtggtcat ctcagacaat gggatgccaa gtcgcacggg caccagcacg 
     5641 ctgaccgtgg ccgtgtgcaa gtgcaacgag cagggcgagt tcaccttctg cgaggatatg 
     5701 gccgcccagg tgggcgtgag catccaggca gtggtagcca tcttactctg catcctcacc 
     5761 atcacagtga tcaccctgct catcttcctg cggcggcggc tccggaagca ggcccgcgcg 
     5821 cacggcaaga gcgtgccgga gatccacgag cagctggtca cctacgacga ggagggcggc 
     5881 ggcgagatgg acaccaccag ctacgatgtg tcggtgctca actcggtgcg ccgcggcggg 
     5941 gccaagcccc cgcggcccgc gctggacgcc cggccttccc tctatgcgca ggtgcagaag 
     6001 ccaccgaggc acgcgcctgg ggcacacgga gggcccgggg agatggcagc catgatcgag 
     6061 gtgaagaagg acgaggcgga ccacgacggc gacggccccc cctacgacac gctgcacatc 
     6121 tacggctacg agggctccga gtccatagcc gagtccctca gctccctggg caccgactca 
     6181 tccgactctg acgtggatta cgacttcctt aacgactggg gacccaggtt taagatgctg 
     6241 gctgagctgt acggctcgga cccccgggag gagctgctgt ataagggtgg gcgcgccgac 
     6301 ccagctttct tgtacaaagt ggtaccggtc atggtgtcta agggcgaaga gctgatcaag 
     6361 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc 
     6421 acaggtgaag gagaaggcag accgtacgag ggagtgcaaa ccatgaggat caaagtcatc 
     6481 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc 
     6541 cgtaccttta tcaagtaccc ggccgacatc cctgatttct ttaaacagtc ctttcctgag 
     6601 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcacgcag 
     6661 gacaccagcc ttgaggatgg cgagctcgtc tacaacgtca aggtcagagg ggtaaacttt 
     6721 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg 
     6781 atgtatccag cagatggtgg tctgagagga tacactgaca tcgcactgaa agttgatggt 
     6841 ggtggccatc tgcactgcaa cttcgtgaca acttacaggt caaaaaagac cgtcgggaac 
     6901 atcaagatgc ccggtgtcca tgccgttgat caccgcctgg aaaggatcga ggagagtgac 
     6961 aatgaaacct acgtagtgca aagagaagtg gcagttgcca aatacagcaa ccttggtggt 
     7021 ggcatggacg agctgtacaa gtaagcggcc gctctagagt cgacgggccg cggtaacaat 
     7081 tgttaactaa cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc 
     7141 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     7201 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     7261 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     7321 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     7381 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     7441 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     7501 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     7561 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt 
     7621 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     7681 aaaacacgat aataccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 
     7741 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 
     7801 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 
     7861 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 
     7921 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 
     7981 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 
     8041 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 
     8101 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 
     8161 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 
     8221 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 
     8281 caagcccggt gcctgacccg ggcggggcgc gtctggaaca atcaacctct ggattacaaa 
     8341 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 
     8401 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 
     8461 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 
     8521 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 
     8581 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 
     8641 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     8701 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 
     8761 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 
     8821 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 
     8881 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa 
     8941 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag 
     9001 aagcacaaga ggaggaggag gtgggttttt ccagtcacac ctcaggtacc tttaagacca 
     9061 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 
     9121 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 
     9181 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 
     9241 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 
     9301 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 
     9361 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 
     9421 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 
     9481 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 
     9541 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 
     9601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 
     9661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 
     9721 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 
     9781 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 
     9841 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     9901 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     9961 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
    10021 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
    10081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
    10141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
    10201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
    10261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
    10321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
    10381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
    10441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
    10501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
    10561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
    10621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
    10681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
    10741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
    10801 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
    10861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
    10921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
    10981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
    11041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
    11101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
    11161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
    11221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
    11281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
    11341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
    11401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
    11461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
    11521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
    11581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
    11641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
    11701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
    11761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
    11821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
    11881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
    11941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
    12001 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt 
    12061 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
    12121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
    12181 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 
    12241 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt 
    12301 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga 
    12361 aattgtattt gttaaatatg tactacaaac ttagtagt 
//
