LOCUS       P1087_mCherry-FKBP-ARHGEF1(380-630)        6661 bp    DNA     circular     16-JUL-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|739641485|
COMMENT     VNTDBDATE|739641485|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    18..381
                     /vntifkey="21"
                     /label=CMV\enhancer
     intron          662..1625
                     /vntifkey="15"
                     /label=Chicken\beta-actin\intron
     promoter        382..661
                     /vntifkey="29"
                     /label=Chicken\beta-actin\promoter
     intron          1626..1718
                     /vntifkey="15"
                     /label=Rabbit\globin\intron
     polyA_signal    3608..4142
                     /vntifkey="25"
                     /label=Rabbit\globin\polyA
     rep_origin      4333..4669
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      4880..5514
                     /vntifkey="33"
                     /label=ColiE1\ori
     CDS             complement(5671..6531)
                     /vntifkey="4"
                     /label=AmpR
     promoter        18..1718
                     /vntifkey="29"
                     /label=CAG\promoter
     CDS             1725..2432
                     /vntifkey="4"
                     /label=mCherry
     CDS             2445..2765
                     /vntifkey="4"
                     /label=FKBP
     CDS             2844..3599
                     /vntifkey="4"
                     /label=ARHGEF1(380-630)
BASE COUNT     1406 a      1824 c      1934 g      1497 t 
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcatggtg agcaagggcg 
     1741 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 
     1801 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 
     1861 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 
     1921 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 
     1981 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 
     2041 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca 
     2101 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 
     2161 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 
     2221 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 
     2281 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 
     2341 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 
     2401 actccaccgg cggcatggac gagctgtaca agagtgctgg tggtggagtg caggtggaaa 
     2461 ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact 
     2521 acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct 
     2581 ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga 
     2641 tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg 
     2701 ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac 
     2761 tggaaagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg 
     2821 gtggtcctcg aggtgcggcc gccgaggggg agccggggcg gtcgggactg gagcttgaac 
     2881 cagaagagcc tcccggctgg cgggaactcg tccccccaga caccctgcac agcctgccca 
     2941 agagccaggt gaagcggcag gaggtcatca gcgagctgct ggtgacagag gcggcccacg 
     3001 tgcgcatgct gcgggtgctg cacgacctct tcttccagcc catggcagaa tgcctgttct 
     3061 tccccttgga ggagctgcag aacatcttcc ccagcctgga cgagctcatc gaggtgcatt 
     3121 ccctgttcct cgatcgcctg atgaagcgga ggcaggagag tggctacctc atcggggaga 
     3181 tcggagacgt gctgctggcc cggtttgatg gtgctgaggg ctcctggttc cagaaaatct 
     3241 cctcccgctt ctgcagccgc cagtcatttg ccttagagca gctcaaagcc aagcaacgca 
     3301 aggaccctcg gttctgtgcc ttcgtgcagg aagctgagag ccgcccgcgg tgccgccgcc 
     3361 tgcagctgaa ggacatgatc cccacggaga tgcagcggct gaccaagtac cccctgctcc 
     3421 tgcagagcat cgggcagaac acagaagcgc ccacagaacg ggagaaagtg gagctggcag 
     3481 ccgagtgctg ccgggaaatt ctacaccacg tcaaccaagc cgtgcgtgac atggaggacc 
     3541 tgctgaggct caaggactat cagcggcgcc tggacttgtc ccaccttcgg cagagctgag 
     3601 cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc 
     3661 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 
     3721 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 
     3781 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 
     3841 acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg 
     3901 aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct 
     3961 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat 
     4021 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc 
     4081 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc 
     4141 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     4201 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     4261 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     4321 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     4381 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     4441 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     4501 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa 
     4561 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     4621 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat 
     4681 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     4741 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     4801 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     4861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     4921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     4981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     5041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     5101 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     5161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     5221 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     5281 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     5341 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     5401 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     5461 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     5521 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     5581 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     5641 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     5701 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     5761 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     5821 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     5881 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     5941 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     6001 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     6061 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     6121 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     6181 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     6241 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     6301 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     6361 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     6421 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     6481 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     6541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     6601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     6661 g 
//
