LOCUS       Exported                7761 bp ds-DNA     circular SYN 31-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRS405-LEU2-TEF-KpolDcr1v2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 31, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(201..2002)
                     /note="origin + beta-lactamase"
     misc_feature    2280..2680
                     /note="TEF2 promoter"
     misc_feature    2639..2661
                     /note="forward seq"
     CDS             2699..4375
                     /codon_start=1
                     /note="Kpol-Dcr1"
                     /translation="MSDQVYVPPTSAPMSNELKVREFYRLHNACVKLKESIKLIYENPL
                     VTDQNVLNLGTAENTIDYTILNTPTLNVAKTLLGNRYSLDLIDLFQSHDFKDSNTDVDM
                     FIKYPVVYDENLENLAFMHKSFPNMNAHLNDAQKTQLSNERLEFLGDSWLGALVSYIVY
                     TRFPSANEGMLSQMKESIVNNNNLFDWSTKLNFTKRLQGNIATPTRVVKDKMSKRYADC
                     VEAYIGALVIDRFGTEFLDIKEWLEELSEKKLAKSSQMVIKEPLNKNAKNELAELLQIN
                     KLGHKLHYRKLTEMPPFRVEVKIGDILLDEAEGNSIREAEHRAAMKVLENDELLRKYSV
                     YDLEDGNSKKYSIEETDLKHDIESSGISNENFLQNEGNGTENIESKFKQLNISNDSNKD
                     DLVNNITNSVFSKLQDVVSKIVIDAISESIPNNPHKIEQSRNGKVGTQTNTSATTSDVL
                     SVQTSIPKSESTSADKAPITCDPNAKQALYTLLSRSQVFPDYEMVQHSMDNFTANCIIK
                     GSGIFLASGRGRSKKIAQQNAASAALRSPILKSILEEQNNF"
     misc_feature    2702..2723
                     /note="KpD7"
     misc_feature    3203..3225
                     /note="KpD3"
     misc_feature    3402..3422
                     /note="KpD5"
     misc_feature    3529..3552
                     /note="KpD6"
     misc_feature    3728..3748
                     /note="KpD4"
     misc_feature    4353..4375
                     /note="KpD8"
     misc_feature    4382..4633
                     /note="CYC1 terminator"
     misc_feature    4432..4452
                     /note="reverse seq"
     misc_feature    5348..7575
                     /note="LEU2"
     misc_feature    6005..6007
                     /note="START"
     misc_feature    6209..6211
                     /note="GTT-->GCT in leu2-3,112"
     misc_feature    6253
                     /note="insertion in leu2-3,112"
     misc_feature    6796
                     /note="insertion in leu2-3,112"
     misc_feature    6902..6904
                     /note="GAC-->AAC in leu2-3,112"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctca
     2281 tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat
     2341 cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct
     2401 agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt
     2461 ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt
     2521 ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt
     2581 cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct
     2641 tgctcattag aaagaaagca tagcaatcta atctaagttt tctagaacta gtggatccat
     2701 gtctgatcaa gtttatgttc cgccaacatc agcaccaatg agcaacgagt taaaagttag
     2761 agaattttat agactccaca atgcttgtgt gaaattgaaa gagtctatta aattgattta
     2821 tgaaaaccca ttggtaactg accaaaatgt tttgaatttg ggaactgcag aaaatactat
     2881 tgattataca attttaaaca cacctacact aaatgttgct aaaactttac tgggcaatag
     2941 atattcatta gatctaatag atctttttca atctcatgat ttcaaagatt caaacactga
     3001 tgtggatatg tttattaaat atcctgtggt ttatgatgaa aatttggaaa atttagcatt
     3061 tatgcataaa agttttccaa atatgaatgc acatctaaat gatgctcaaa agactcaatt
     3121 atctaatgaa aggttagaat ttttgggtga tagttggcta ggtgctttag tttcatatat
     3181 tgtatacacc agatttcctt ctgcaaatga aggtatgctt tctcaaatga aagaatcaat
     3241 agtaaataat aataatctat ttgattggag tacaaaatta aacttcacca agagattaca
     3301 aggtaatatc gctacaccta ctagagtggt caaagataaa atgtctaaac gttatgctga
     3361 ttgtgttgaa gcctatattg gtgcattggt tattgataga tttggcactg agtttttgga
     3421 tatcaaggaa tggttagaag aactatcaga aaaaaaattg gccaaatcga gccaaatggt
     3481 gataaaggaa ccattaaata aaaatgctaa aaatgaatta gccgaattac tgcaaattaa
     3541 caaattagga cataaacttc attacagaaa gcttacagaa atgcctccat tcagagttga
     3601 agttaaaata ggtgacattc tgttagacga agctgaaggt aacagtataa gggaagcaga
     3661 acacagagct gcgatgaagg tattggaaaa tgatgaatta ttgaggaaat attcggttta
     3721 tgatttagag gatggaaact cgaagaaata ttctattgaa gagacggatc ttaaacatga
     3781 tattgaatcc tctggaatat ctaatgaaaa ttttttgcaa aatgagggaa atggaactga
     3841 aaatatagaa tcaaaattta aacaattgaa tatatcaaat gatagcaaca aagatgattt
     3901 agttaataat attacaaatt ctgtttttag taaattgcag gatgtcgtaa gtaaaattgt
     3961 cattgacgct atttccgaat caatcccaaa taatccccac aaaatagaac aatctagaaa
     4021 tggtaaagta ggaacacaaa caaatactag tgctacaact tcagatgtcc tttcagttca
     4081 aacgtctatt cctaaaagcg agtcaacttc ggctgataaa gctcctatca catgtgatcc
     4141 caatgccaaa caggcattgt ataccctgtt atctagatca caagtgttcc cagactatga
     4201 aatggttcaa cattccatgg ataatttcac cgctaactgt atcattaaag gcagcggtat
     4261 atttttagcg tccggaagag gtcgcagtaa gaagattgct cagcaaaatg cagcatcagc
     4321 ggcacttcgg agcccaatct taaaatctat tttggaagaa caaaacaatt tttaactcga
     4381 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     4441 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     4501 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     4561 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     4621 ttaatttgcg gccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     4681 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4741 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4801 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     4861 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4921 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4981 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5041 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5101 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5161 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5221 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     5281 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5341 tcgacggtcg aggagaactt ctagtatatc cacataccta atattattgc cttattaaaa
     5401 atggaatccc aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact
     5461 tccttgttca tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca
     5521 acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac
     5581 cgcagttaac tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca
     5641 accattattt ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa
     5701 attcgatgac tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc
     5761 aactgaaaaa ttgggagaaa aaggaaaggt gagaggccgg aaccggcttt tcatatagaa
     5821 tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt
     5881 aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt
     5941 accttttaca tttcagcaat atatatatat atttcaagga tataccattc taatgtctgc
     6001 ccctatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat
     6061 cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt
     6121 cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg tcccacttcc
     6181 agatgaggcg ctggaagcct ccaagaaggt tgatgccgtt ttgttaggtg ctgtggctgg
     6241 tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga
     6301 acttcaattg tacgccaact taagaccatg taactttgca tccgactctc ttttagactt
     6361 atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt
     6421 gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag
     6481 tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct
     6541 acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatcttt tggcctcttc
     6601 aagattatgg agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt
     6661 tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa
     6721 tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat
     6781 cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac
     6841 cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt
     6901 tgaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc
     6961 tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac
     7021 tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt
     7081 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat
     7141 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag
     7201 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatagtaa
     7261 aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca
     7321 ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa
     7381 atcatgaaac tacgattcct aatttgatat tggaggattt tctctaaaaa aaaaaaaata
     7441 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct
     7501 tgaagcattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc
     7561 ttacgacgta gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7621 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7681 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7741 cgtcatcacc gaaacgcgcg a
//
