LOCUS       Exported                7167 bp ds-DNA     circular SYN 03-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1zeo+GagCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 3, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2457..3111
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
     promoter        209..863
                     /label=CMV promoter
                     /note="CMV promoter"
     promoter        863..882
                     /label=T7 promoter priming site
                     /note="T7 promoter priming site promoter"
     misc_feature    895..900
                     /note="NheI"
     misc_feature    911..916
                     /note="HindIII"
     misc_feature    911
                     /note="HindIII"
     CDS             923..3136
                     /codon_start=1
                     /label=GagCherry
                     /note="GagCherry"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIDVKDTKEALEKI
                     EEEQNKSKKKAQQAAAAAGTGNSSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEE
                     KAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHA
                     GPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSP
                     TSILDIRQGPKEPFRDYVDRFYKTLRAEQASQDVKNWMTETLLVQNANPDCKTILKALG
                     PAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMMQRGNFRNQRKTVKCFNCGK
                     EGHTARNCRAPRKKGCWRCGREGHQMKDCTERQANFLGKIWPSYKGRPGNFLQSRPEPT
                     APPEESFRFGEEKTTPSQKQEPIDKELYPLTSLRSLFGNDPSSQGSKGEEDNMAIIKEF
                     MRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSK
                     AYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNF
                     PSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQ
                     LPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     misc_feature    2429..2434
                     /note="BamHI"
     misc_feature    2457..3111
                     /product="monomeric red fluorescent protein"
                     /note="mCherry; engineered variant of monomeric redfluores 
                     cent protein mRFP1 in GenBank Accession NumberAF506027; N- 
                     and C-termini replaced with equivalentresidues from EGFP"
                     /db_xref="GI:55420613"
     misc_feature    3138..3142
                     /note="XhoI"
     misc_feature    3143..3148
                     /note="XbaI"
     misc_feature    3149..3154
                     /note="ApaI"
     polyA_signal    3173..3387
                     /label=BGH polyA
                     /note="BGH polyA"
     rep_origin      3450..3863
                     /label=f1 origin
                     /note="f1 origin"
     rep_origin      3928..4253
                     /label=SV40 promoter and origin
                     /note="SV40 promoter and origin"
     CDS             4336..4710
                     /codon_start=1
                     /label=Zeocin
                     /note="Zeocin"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4840..4969
                     /label=SV40 polyA
                     /note="SV40 polyA"
     rep_origin      5353..6026
                     /label=pUC origin
                     /note="pUC origin"
     CDS             complement(6171..7031)
                     /codon_start=1
                     /label=Amlicillin (bla) resistance gene
                     /note="Amlicillin (bla) resistance gene"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7032..7130)
                     /label=bla promoter
                     /note="bla promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgggcgc ccgcgccagc gtgctgagcg gcggcgagct
      961 ggacaagtgg gagaagatcc gcctgcgccc cggcggcaag aagaagtaca agctgaagca
     1021 catcgtgtgg gccagccgcg agctggagcg cttcgccgtg aaccccggcc tgctggagac
     1081 cagcgagggc tgccgccaga tcctgggcca gctgcagccc agcctgcaga ccggcagcga
     1141 ggagctgcgc agcctgtaca acaccgtggc caccctgtac tgcgtgcacc agcgcatcga
     1201 cgtcaaggac accaaggagg ccctggagaa gatcgaggag gagcagaaca agtccaagaa
     1261 gaaggcccag caggccgccg ccgccgccgg caccggcaac agcagccagg tgagccagaa
     1321 ctaccccatc gtgcagaacc tgcagggcca gatggtgcac caggccatca gcccccgcac
     1381 cctgaacgcc tgggtgaagg tggtggagga gaaggccttc agccccgagg tgatccccat
     1441 gttcagcgcc ctgagcgagg gcgccacccc ccaggacctg aacacgatgt tgaacaccgt
     1501 gggcggccac caggccgcca tgcagatgct gaaggagacc atcaacgagg aggccgccga
     1561 gtgggaccgc gtgcaccccg tgcacgccgg ccccatcgcc cccggccaga tgcgcgagcc
     1621 ccgcggcagc gacatcgccg gcaccaccag caccctgcag gagcagatcg gctggatgac
     1681 caacaacccc cccatccccg tgggcgagat ctacaagcgg tggatcatcc tgggcctgaa
     1741 caagatcgtg cggatgtaca gccccaccag catcctggac atccgccagg gccccaagga
     1801 gcccttccgc gactacgtgg accgcttcta caagaccctg cgcgctgagc aggccagcca
     1861 ggacgtgaag aactggatga ccgagaccct gctggtgcag aacgccaacc ccgactgcaa
     1921 gaccatcctg aaggctctcg gccccgcggc caccctggag gagatgatga ccgcctgcca
     1981 gggcgtgggc ggccccggcc acaaggcccg cgtgctggcc gaggcgatga gccaggtgac
     2041 gaacccggcg accatcatga tgcagcgcgg caacttccgc aaccagcgga agaccgtcaa
     2101 gtgcttcaac tgcggcaagg agggccacac cgccaggaac tgccgcgccc cccgcaagaa
     2161 gggctgctgg cgctgcggcc gcgagggcca ccagatgaag gactgcaccg agcgccaggc
     2221 caacttcctg ggcaagatct ggcccagcta caagggccgc cccggcaact tcctgcagag
     2281 ccgccccgag cccaccgccc cccccgagga gagcttccgc ttcggcgagg agaagaccac
     2341 ccccagccag aagcaggagc ccatcgacaa ggagctgtac cccctgacca gcctgcgcag
     2401 cctgttcggc aacgacccca gcagccaggg atccaagggc gaggaggata acatggccat
     2461 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     2521 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     2581 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     2641 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     2701 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     2761 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     2821 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2881 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     2941 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     3001 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     3061 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     3121 cgagctgtac aagtaactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     3181 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3241 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3301 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3361 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3421 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3601 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3661 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3781 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     3841 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     3901 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     3961 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4021 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4141 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4201 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4261 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     4321 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     4381 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     4441 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     4501 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     4561 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     4621 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     4681 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     4741 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4801 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     4861 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4921 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     4981 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5041 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5101 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5161 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5221 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5581 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     5641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5821 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     5881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7141 tttccccgaa aagtgccacc tgacgtc
//
