LOCUS       pAAV_hSyn1_bPAC\cMyc\T2A\tDimer        7176 bp    DNA     circular      1-NOV-2017
DEFINITION  .
SOURCE      .
  ORGANISM  .
COMMENT     
            ApEinfo:methylated:0
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11919871|
COMMENT     VNTDBDATE|-12008838|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV_hSyn1_bPAC cMyc T2A tDimer|
COMMENT     VNTAUTHORNAME|Iris Ohmert|
COMMENT     VNTAUTHORTEL|004940741055085|
COMMENT     VNTAUTHORFAX|004940741058364|
COMMENT     VNTAUTHOREML|iohmert@zmnh.uni-hamburg.de|
COMMENT     VNTAUTHORAD1|Zentrum fuer Molekulare Neurobiologie Hamburg|
COMMENT     VNTAUTHORAD2|Institut fuer Synaptische Physiologie|
COMMENT     VNTAUTHORAD3|Falkenried 94/Martinistr.85|
COMMENT     VNTAUTHORAD4|D-20251 Hamburg|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    489..1580
                     /ApEinfo_fwdcolor=#8dbd4a
                     /ApEinfo_revcolor=#8dbd4a
                     /vntifkey="21"
                     /label=bPAC
     polyA_signal    3063..3077
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /vntifkey="25"
                     /label=SV40
     misc_feature    3086..3681
                     /ApEinfo_fwdcolor=#ff629d
                     /ApEinfo_revcolor=#ff629d
                     /vntifkey="21"
                     /label=WRE
                     /note="Oriinal location: 2863..3458"
     CDS             1668..3062
                     /ApEinfo_fwdcolor=#ff9b79
                     /ApEinfo_revcolor=#ff9b79
                     /vntifkey="4"
                     /label=tdimer2
     polyA_signal    3763..4240
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /vntifkey="25"
                     /label=hGH\polyA\Signal
                     /note="Oriinal location: 3533..4010"
     CDS             1593..1652
                     /ApEinfo_fwdcolor=#59ff62
                     /ApEinfo_revcolor=#59ff62
                     /vntifkey="4"
                     /label=T2A
     misc_signal     4281..4420
                     /ApEinfo_fwdcolor=#3f3cff
                     /ApEinfo_revcolor=#3f3cff
                     /vntifkey="87"
                     /label=right\ITR
                     /note="Oriinal location: 4051..4190"
     rep_origin      4513..4818
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /vntifkey="33"
                     /label=f1\origin
                     /note="Oriinal location: 4283..4588"
     misc_structure  5338..6191
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /vntifkey="88"
                     /label=amp\resistance
                     /note="Oriinal location: 5108..5961"
     rep_origin      6346..7012
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="Oriinal location: 6116..6782"
     repeat_unit     7019..7158
                     /ApEinfo_fwdcolor=#5156ff
                     /ApEinfo_revcolor=#5156ff
                     /vntifkey="35"
                     /label=left\ITR
                     /note="Oriinal location: 6789..6928"
     misc_feature    6..474
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /vntifkey="21"
                     /label=hSyn1_Promotor
     misc_feature    1551..1580
                     /vntifkey="21"
                     /label=c\Myc
BASE COUNT     1544 a      2147 c      1982 g      1503 t 
ORIGIN
        1 ctagactgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg 
       61 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt 
      121 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg 
      181 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc 
      241 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc 
      301 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg 
      361 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg 
      421 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat 
      481 tcgccaccat gatgaagcgg ctggtgtaca tcagcaagat cagcggccac ctgagcctgg 
      541 aagagatcca gcggatcggc aaggtgtcca tcaagaacaa ccagcgggac aacatcaccg 
      601 gcgtgctgct gtacctgcag ggcctgttct tccagatcct ggaaggcgag aacgagaagg 
      661 tggacaagct gtacaagaaa atcctggtgg acgaccggca caccaacatc ctgtgcctga 
      721 aaaccgagta cgacatcacc gaccggatgt tccccaactg ggccatgaaa accatcaacc 
      781 tgaacgagaa cagcgagctg atgatccagc ccatcaagag cctgctgcag accatcaccc 
      841 agagccaccg ggtgctggaa aagtacatgc ccgccagagt gatctacctg atcaaccagg 
      901 gcatcaaccc cctgaccgtg gagccccagc tggtcgagaa gatcatcttc ttcagcgaca 
      961 tcctggcctt cagcaccctg accgagaagc tgcccgtgaa cgaggtggtc atcctggtca 
     1021 accggtactt cagcatctgc acccggatca tcagcgccta cggcggcgaa gtgaccaagt 
     1081 tcatcggcga ctgcgtgatg gccagcttca ccaaagagca gggcgacgcc gccatccgga 
     1141 ccagcctgga catcatctcc gagctgaagc agctgcggca ccacgtggag gccaccaacc 
     1201 ccctgcacct gctgtacacc ggcatcggcc tgagctacgg ccacgtgatc gagggcaaca 
     1261 tgggcagcag cctgaagatg gaccacaccc tgctggggga cgccgtgaac gtggccgcca 
     1321 ggctggaagc cctgacaaga cagctgccct acgccctggc ctttacagcc ggcgtgaaga 
     1381 agtgctgcca ggcccagtgg accttcatca acctgggagc ccaccaggtc aagggcaagc 
     1441 aggaagccat cgaggtctac accgtgaatg aggcccagaa gtactacgac accctgcaga 
     1501 tcacccagct gatccggcag accctggaaa acgacaagcc gcggacgtac gagcaaaagc 
     1561 tgatctcgga ggaggacctg gtacccgcgg ccgagggcag aggaagtctt ctaacatgcg 
     1621 gtgacgtgga ggagaatccc ggccctgcac cgggatccgc ggccgccatg gtggcctcct 
     1681 ccgaggacgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg 
     1741 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 
     1801 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc 
     1861 agttccagta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gactacaaga 
     1921 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 
     1981 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaagt 
     2041 tccgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg 
     2101 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg 
     2161 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca 
     2221 agaagcccgt gcagctgccc ggctactact acgtggacac caagctggac atcacctccc 
     2281 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt 
     2341 tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg 
     2401 acgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg 
     2461 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 
     2521 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc 
     2581 agtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt 
     2641 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga 
     2701 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagttccgcg 
     2761 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct 
     2821 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga 
     2881 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc 
     2941 ccgtgcagct gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg 
     3001 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt 
     3061 aggtcgactt cgagcaaaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 
     3121 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     3181 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     3241 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     3301 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     3361 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     3421 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     3481 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     3541 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     3601 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     3661 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cgcgatcgcg gcgcgccatt 
     3721 cgatggcctc tctggcctcg acctcgagtc tagagatgat ctacgggtgg catccctgtg 
     3781 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg 
     3841 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg 
     3901 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg 
     3961 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg 
     4021 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat 
     4081 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg 
     4141 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc 
     4201 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca 
     4261 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg 
     4321 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg 
     4381 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt 
     4441 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg 
     4501 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     4561 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     4621 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     4681 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg 
     4741 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     4801 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg 
     4861 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     4921 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct 
     4981 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 
     5041 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 
     5101 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 
     5161 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 
     5221 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     5281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     5341 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     5401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     5461 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     5521 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     5581 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     5641 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     5701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     5761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     5821 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     5881 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     5941 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     6001 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     6061 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     6121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     6181 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     6241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     6301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     6361 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     6421 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     6481 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 
     6541 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     6601 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     6661 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     6721 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     6781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     6841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     6901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     6961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc 
     7021 tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg 
     7081 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac 
     7141 tccatcacta ggggttcctg cggccgcacg cgtcga 
//
