LOCUS       Exported               12876 bp ds-DNA     circular SYN 15-NOV-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pHR_Gal4UAS_IRES_mC_pGK_tBFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Kole Roybal
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2016 from SnapGene 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="match to pHR-CMVLacZ"
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="SP6"
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla (AmpR)
                     /note="bla"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="AmpR"
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen
                     /note="SV40 - Invitrogen promoter"
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="SV40 pA"
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="SV40 late polyA"
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="SV40 early poly A"
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A (2)
                     /note="SV40 early poly A"
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="cPPT"
                     /note="color: #a6acb3"
     primer_bind     7673..7696
                     /note="cPPT_5'seq"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    7722..7807
                     /note="Gal4"
                     /note="color: #99ccff"
     misc_feature    7848..7853
                     /note="SpeI"
                     /note="color: #a6acb3"
     promoter        7854..7971
                     /note="mCMV"
                     /note="color: #ffffff"
     misc_feature    7992..8000
                     /note="KOZAK"
                     /note="color: #a6acb3"
     CDS             8001..9623
                     /codon_start=1
                     /note="color: #000000"
                     /translation="MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEM
                     VVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLL
                     HKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSS
                     DPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYEN
                     YTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKS
                     KREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCSGGGGSGGGGSGG
                     GGSRNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDK
                     TSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVE
                     FKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLC
                     ILLHAFRIRAVTIDRVMSYLNAS"
     misc_feature    8001..8984
                     /note="IL-12 p40"
                     /note="color: #33cccc"
     misc_feature    8985..9029
                     /note="3x GGGGS Linker"
                     /note="color: #a6acb3"
     misc_feature    9030..9623
                     /note="IL-12 p35"
                     /note="color: #00ffff"
     misc_feature    9642..10215
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
                     /note="color: #a6acb3"
     CDS             10234..10941
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    10957..10963
                     /label=AarI
                     /note="AarI"
                     /note="color: #ff0080"
     misc_feature    10968..10971
                     /label=Part D
                     /note="Part D"
                     /note="color: #00ffff"
     misc_feature    11029..11528
                     /note="promPGK"
                     /note="color: #996633; direction: RIGHT"
     CDS             11549..12247
                     /codon_start=1
                     /gene="TagBFP"
                     /note="TagBFP"
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     enhancer        12285..12876
                     /label=WPRE
                     /note="WPRE"
                     /note="color: #a6acb3"
     misc_feature    complement(12308..12331)
                     /label=cw11
                     /note="cw11"
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     12339..12359
                     /label=QB0112 WPRE-R binding site
                     /note="QB0112 WPRE-R binding site"
                     /note="color: #00ffff; direction: RIGHT"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
     7741 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
     7801 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
     7861 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     7921 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
     7981 ttcgttggat cgccgccacc atgtgtcatc agcaactggt cattagctgg ttttccttgg
     8041 tgttcctggc ctcacctctc gttgcaattt gggaactgaa gaaagacgta tatgtcgtgg
     8101 aactggactg gtatcccgat gccccagggg aaatggtggt ccttacttgc gacacccccg
     8161 aagaggacgg cattacatgg acccttgacc agtccagtga ggtcctcggg agtggaaaga
     8221 cactgacaat tcaggttaag gagttcggcg atgccggtca gtacacctgt cataaagggg
     8281 gagaggttct gtcccattca ctcctgctgc tgcacaagaa ggaagatggt atctggtcca
     8341 cggacatttt gaaagatcaa aaggagccca agaacaaaac tttcctgcgg tgtgaagcca
     8401 aaaattacag cgggcgattt acttgttggt ggctcaccac tattagtaca gatctcacct
     8461 tcagcgttaa gagttctagg ggctccagtg atccgcaggg agtgacgtgt ggcgctgcga
     8521 ccctgtccgc tgagcgagtg aggggcgata ataaggagta tgagtatagt gtggagtgtc
     8581 aggaggattc tgcttgccct gctgccgagg agtcccttcc tatcgaagtg atggtcgatg
     8641 ctgtacacaa actgaaatac gagaattata ctagctcctt tttcataagg gacatcataa
     8701 agccagatcc ccccaagaac cttcagctga aaccgctgaa aaattcacgc caggtggagg
     8761 tatcttggga gtacccagac acatggagca cacctcatag ttacttcagc ctgaccttct
     8821 gtgtccaggt ccaggggaag tctaaaaggg aaaaaaagga cagagttttc accgacaaga
     8881 catccgcaac cgtaatctgt aggaaaaacg cctcaatttc cgttagagcg caagataggt
     8941 attattcctc ttcctggtct gaatgggcct ctgtaccctg cagcgggggt ggcggtagcg
     9001 gtgggggagg gagcggaggt ggaggcagcc gaaacctgcc tgtggccaca cctgatcctg
     9061 ggatgttccc ttgcttgcac cacagtcaga atctgctcag ggccgttagc aacatgctgc
     9121 agaaggcgcg gcagacactt gagttctacc cttgcacaag tgaagagatc gatcacgaag
     9181 atatcacaaa ggataaaacg tctactgtcg aggcctgcct ccccctcgaa ctcacaaaga
     9241 acgagagttg cctcaattcc cgggaaacca gcttcatcac taatgggtcc tgcctggcaa
     9301 gcagaaaaac ttctttcatg atggcattgt gtttgagtag catctatgag gacctcaaaa
     9361 tgtaccaagt tgaatttaag actatgaacg ctaagctcct catggaccct aagcggcaga
     9421 tcttcctgga tcagaacatg ttggctgtca tcgacgaact catgcaagcc ctgaacttta
     9481 attccgaaac agttccccag aagtccagtc tggaggaacc cgatttctac aaaaccaaaa
     9541 ttaagctgtg tatcttgctg catgctttcc ggatcagggc cgttacgatc gatcgggtaa
     9601 tgagctacct gaatgccagt taggatcctt gacttgcggc ccccctctcc ctcccccccc
     9661 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     9721 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     9781 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     9841 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     9901 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     9961 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
    10021 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
    10081 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
    10141 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
    10201 aaaacacgat gataaatgag cgctggcggg tccatggtga gcaagggcga ggaggataac
    10261 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
    10321 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
    10381 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
    10441 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
    10501 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
    10561 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
    10621 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
    10681 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
    10741 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
    10801 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
    10861 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
    10921 ggcatggacg agctgtacaa gtagggccgc aactcccacc tgcaacatgc gtgactgact
    10981 gaggccgcga ctctagagtc gacctgcacg aggttaacga attctaccgg gtaggggagg
    11041 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
    11101 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
    11161 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
    11221 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
    11281 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
    11341 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
    11401 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
    11461 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
    11521 gcctttcgac ctgcagccca agcttaccat gagcgagctg attaaggaga acatgcacat
    11581 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
    11641 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
    11701 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
    11761 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
    11821 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
    11881 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
    11941 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
    12001 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
    12061 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
    12121 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
    12181 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
    12241 gcttaattaa tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
    12301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    12361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    12421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    12481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    12541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    12601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    12661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    12721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    12781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    12841 acgagtcgga tctccctttg ggccgcctcc ccgcat
//
