LOCUS       pZS1-lTlrLLtCL-t        8703 bp    DNA     linear       11-NOV-2016
FEATURES             Location/Qualifiers
     source          4005..12707
                     /dnas_title="pZS1-lTlrLLtCL-tet-mVenus-pRNA1-mSCFP3nodegTa
                     g"
                     /organism="repressilator (pZS1-lTlrLLtCL)"
                     /mol_type="other DNA"
     CDS             complement(7703..8653)
                     /label=ORF frame 3
                     /note="ORF frame 3"
     gene            complement(836..1696)
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI*"
     CDS             complement(836..1696)
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(1738..1766)
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
     promoter        complement(2038..2769)
                     /note="pBR322 RNA Promoter from pPM60"
     CDS             complement(2053..2769)
                     /note="mSCFP3 from Cluzel lab"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNG
                     IKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
     misc_feature    complement(1935..2032)
                     /note="T1 terminator"
     promoter        complement(2812..2817)
                     /note="-10 element"
     promoter        complement(2791..2880)
                     /note="pRNA1 from pBR322"
     misc_feature    complement(2777..2790)
                     /note="RBS"
     promoter        complement(2770..2880)
                     /note="pBR322 RNA Promoter from pPM60"
     CDS             complement(3075..3791)
                     /note="mVenus from cluzel lab, (nat. bio)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG
                     IKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
     terminator      complement(2968..3011)
                     /label=rrnB_T1_terminator
                     /translation="IKRKAQSKDWAFRF"
     misc_feature    complement(2881..2886)
                     /note="SacI"
     promoter        complement(2835..2840)
                     /note="-35 element"
     promoter        complement(3841..3894)
                     /note="tetR promoter"
     gene            4111..4734
                     /label=TetR
                     /note="TetR"
     promoter        4035..4051
                     /note="LacO"
     promoter        4012..4028
                     /note="LacO"
     promoter        4029..4034
                     /note="-35"
     promoter        4052..4057
                     /note="-10"
     promoter        4012..4057
                     /note="pL Lac O1"
     terminator      4764..4807
                     /label=rrnB_T1_terminator
                     /note="rrnB_T1_terminator"
     promoter        4888..4936
                     /note="pR"
     promoter        4888..4903
                     /note="OR2"
     promoter        4901..4906
                     /note="-35"
     promoter        4911..4926
                     /note="OR1"
     promoter        4922..4927
                     /note="-10"
     gene            4974..6065
                     /note="lacI"
                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAA
                     MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMV
                     ERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPIN
                     SIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAE
                     REGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGY
                     DDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA
                     PNTQTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    4986..6053
                     /label=lacI
                     /note="lacI"
     terminator      6095..6138
                     /label=rrnB_T1_terminator
                     /note="rrnB_T1_terminator"
     CDS             6310..7023
                     /note="cI"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     promoter        6209..6262
                     /note="pL tet"
     terminator      7084..7127
                     /label=rrnB_T1_terminator
                     /note="rrnB_T1_terminator"
ORIGIN      
        1 ctagtcaatg ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct
       61 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt
      121 tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa
      181 aaaagataaa aagaatagat cccagccctg tgtataactc actactttag tcagttccgc
      241 agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc
      301 ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct
      361 ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg
      421 gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg
      481 aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt
      541 ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca
      601 gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa
      661 ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc
      721 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      781 cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct gacagttacc
      841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1141 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1441 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1741 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1801 gcacatttcc ccgaaaagtg ccacctgacg tctctttcct gcgttatccc ctgattctgt
     1861 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc cagcctacac
     1921 CCCGGGTCTA GGGCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA
     1981 TAAAACGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTCTAGACTC
     2041 AGCTAATTAA GCTTATTTGT ATAGTTCATC CATGCCATGT GTAATCCCAG CAGCTGTTAC
     2101 AAACTCAAGA AGGACCATGT GGTCTCTCTT TTCGTTGGGA TCTTTCGAAA GCTTAGATTG
     2161 TGTGGACAGG TAATGGTTGT CTGGTAAAAG GACAGGGCCA TCGCCAATTG GAGTATTTTG
     2221 TTGATAATGG TCTGCTAGTT GAACGCCTCC ATCTTCAATG TTGTGTCTAA TTTTGAAGTT
     2281 AGCTTTGATT CCATTCTTTT GTTTGTCTGC CGTGATGTAT ACATTGTCTG AGATGTAGTT
     2341 GTATTCCAAT TTGTGTCCAA GAATGTTTCC ATCTTCTTTA AAATCAATAC CTTTTAACTC
     2401 GATTCTATTA ACAAGGGTAT CACCTTCAAA CTTGACTTCA GCACGTGTCT TGTAGTTCCC
     2461 GTCATCTTTG AAAAATATAG TTCTTTCCTG TACATAACCT TCGGGCATGG CACTCTTGAA
     2521 AAAGTCATGC TGTTTCATAT GATCTGGGTA TCTTGCAAAG CATTGAACAC CCCAAGTGAG
     2581 AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA TAAATTTAAG
     2641 GGTAAGTTTT CCGTATGTTG CATCACCTTC ACCCTCTCCA CTGACAGAAA ATTTGTGCCC
     2701 ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC
     2761 TTTACTCATg gatccatctc cttcttaaat gaattCAAAT ACTGTCCTTC TAGTGTAGCC
     2821 GTAGTTAGGC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     2881 GAGCTCGAAT TCACTGGCCG TCGTTTTACA taggtctagg gcggcggatt tgtcctactc
     2941 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     3001 tttcgtttta tttgatgcct ctagcacgcg taccatggga tcccccgggc tgcaggaatt
     3061 cgatatcaag cttaTTATTT GTATAGTTCA TCCATGCCAT GTGTAATCCC AGCAGCTGTT
     3121 ACAAACTCAA GAAGGACCAT GTGGTCTCTC TTTTCGTTGG GATCTTTCGA AAGCTTAGAT
     3181 TGGTAGGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT TGGAGTATTT
     3241 TGTTGATAAT GGTCTGCTAG TTGAACACCT CCATCTTCAA TGTTGTGTCT AATTTTGAAG
     3301 TTCGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG TGAGTTATAG
     3361 TTGTATTCCA ATTTGTGTCC AAGAATGTTT CCATCTTCTT TAAAATCAAT ACCTTTTAAC
     3421 TCGATTCTAT TAACAAGGGT ATCACCTTCA AACTTGACTT CAGCACGTGT CTTGTAGTTC
     3481 CCGTCATCTT TGAAAAATAT AGTTCTTTCC TGTACATAAC CTTCGGGCAT GGCACTCTTG
     3541 AAAAAGTCAT GCTGTTTCAT ATGATCTGGG TATCTCGCAA AGCATTGAAC ACCATAACCC
     3601 AAAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA AATCAATTTA
     3661 AGGGTAAGTT TTCCGTATGT TGCATCACCT TCACCCTCTC CACTGACAGA AAATTTGTGC
     3721 CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA AAGTTCTTCT
     3781 CCTTTACTCA Tgcggtacct ttctcctctt taatgaattc ggtcagtgcg tcctgctgat
     3841 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactcgag
     3901 gtgaagacga aagggcctcg tgctcgagta cgtctaagaa accattatta tcatgacatt
     3961 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgaga aaacactcgc
     4021 ctattgttaa ctgtaacact cgcctattgt tctatgactc gtgtagtcgt cctgcgtgac
     4081 tggcttaagt aatttctcct ctttccatgg tacaggtcta atctattttc atttcactaa
     4141 ttgtcgcgta atctcgacga attactccag ccttagcttc caaattgttg ggcatttgag
     4201 cgggtcttcg atccacatct cgtcggatgt aacataaccg tacatttttt attcgcccga
     4261 aacgagctgc ggaatcggta actctacaat ctatccgtgg tatgagtgaa aacgggaaat
     4321 cttccccttt cgaccgttct aaaaaatgca ttattgcgat tttcaaaatc tacacgaaat
     4381 gattcagtag cgctacctcg ttttcatgta aatccatgtg ccggatgtct ttttgtcata
     4441 ctttgagagc ttttagttaa tcggaaaaat acggttgttc caaaaagtga tctcttacgt
     4501 aatatacgtg agtcgcgaca ccccgtaaaa tgaaatccaa cgcataacct tctagttctc
     4561 gtagttcagc gatttcttct ttccctttgt ggatgatgac tatcatacgg cggtaataat
     4621 gctgttcgat agcttaataa actagtggtt ccacgtctcg gtcggaagaa taagccggaa
     4681 cttaactagt atacgcctaa tctttttgtt gaatttacac tttcacccag aattcgaaat
     4741 cgtcgaatta gatctccgta gtttattttg ctttccgagt cagctttctg acccggaaag
     4801 caaaatagac aacaaacagc cacttgcgag aggactcatc ctgtttaggc ggcgggatct
     4861 ggatcgacgt ccagctccta tttatagatt gtggcacgca caactgataa aatggagacc
     4921 gccactatta ccaacgtaca tgatcttaag taatttctcc tctttccatg gtataccact
     4981 tacactttgg tcattgcaat atgctacagc gtctcatacg gccacagaga atagtctggc
     5041 aaagggcgca ccacttggtc cggtcggtgc aaagacgctt ttgcgccctt tttcaccttc
     5101 gccgctaccg cctcgactta atgtaagggt tggcgcaccg tgttgttgac cgcccgtttg
     5161 tcagcaacga ctaaccgcaa cggtggaggt cagaccggga cgtgcgcggc agcgtttaac
     5221 agcgccgcta atttagagcg cggctagttg acccacggtc gcaccaccac agctaccatc
     5281 ttgcttcgcc gcagcttcgg acatttcgcc gccacgtgtt agaagagcgc gttgcgcagt
     5341 cacccgacta gtaattgata ggcgacctac tggtcctacg gtaacgacac cttcgacgga
     5401 cgtgattaca aggccgcaat aaagaactac agagactggt ctgtgggtag ttgtcataat
     5461 aaaagagggt acttctgcca tgcgctgacc cgcacctcgt agaccagcgt aacccagtgg
     5521 tcgtttagcg cgacaatcgc ccgggtaatt caagacagag ccgcgcagac gcagaccgac
     5581 cgaccgtatt tatagagtga gcgttagttt aagtcggcta tcgccttgcc cttccgctga
     5641 cctcacggta caggccaaaa gttgtttggt acgtttacga cttactcccg tagcaagggt
     5701 gacgctacga ccaacggttg ctagtctacc gcgacccgcg ttacgcgcgg taatggctca
     5761 ggcccgacgc gcaaccacgc ctatagagcc atcaccctat gctgctatgg cttctgtcga
     5821 gtacaatata gggcggcaat tggtggtagt ttgtcctaaa agcggacgac cccgtttggt
     5881 cgcacctggc gaacgacgtt gagagagtcc cggtccgcca cttcccgtta gtcgacaacg
     5941 ggcagagtga ccacttttct ttttggtggg accgcgggtt atgcgtttgg cggagagggg
     6001 cgcgcaaccg gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg
     6061 tcattcgaat taatcgactc agatctccgt agtttatttt gctttccgag tcagctttct
     6121 gacccggaaa gcaaaataga caacaaacag ccacttgcga gaggactcat cctgtttagg
     6181 cggcgggatc tggatcgacg tccagctcag ggatagtcac tatctctaac tgtagggata
     6241 gtcactatct ctatgactcg tgtagtcgtc ctgcgtgact ggcttaagta atttctcctc
     6301 tttccatggt actcgtgttt tttctttggt aattgtgttc tcgtcgaact cctgcgtgca
     6361 gcggaatttc gttaaatact ttttttcttt ttacttgaac cgaatagggt ccttagacag
     6421 cgtctgttct acccctaccc cgtcagtccg caaccacgaa ataaattacc gtagttacgt
     6481 aatttacgaa tattgcggcg taacgaacgt ttttaagagt ttcaatcgca acttcttaaa
     6541 tcgggaagtt agcggtctct ttagatgctc tacatacttc gccaatcata cgtcggcagt
     6601 gaatcttcac tcatactcat gggacaaaaa agagtacaag tccgtcccta caagagtgga
     6661 ctcgaatctt ggaaatggtt tccactacgc ctctctaccc attcgtgttg gttttttcgg
     6721 tcactaagac gtaagaccga actccaactt ccattaaggt actggcgtgg ttgtccgagg
     6781 ttcggttcga aaggactgcc ttacaattaa gagcaactgg gactcgtccg acaactcggt
     6841 ccactaaaga cgtatcggtc tgaaccccca ctactcaaat ggaagttctt tgactagtcc
     6901 ctatcgccag tccacaaaaa tgttggtgat ttgggtgtca tgggttacta gggtacgtta
     6961 ctctcaacaa ggcaacaccc ctttcaatag cgatcagtca ccggacttct ctgcaaaccg
     7021 acttttgatg cgaaatcgtc gaactagatc tcctagggta ccatgcgcac gatctccgta
     7081 gtttattttg ctttccgagt cagctttctg acccggaaag caaaatagac aacaaacagc
     7141 cacttgcgag aggactcatc ctgtttaggc ggcgggatct ggatcccatg cccaaaacga
     7201 cgggcgtttg cccgacaaga ccacaacgat caaacaatag tcttagcgtc taggccgaag
     7261 tccaaacggc cgactttcgc gataaagaag gtcttaacgg tactaaaaaa ggggtgccct
     7321 ccgcagtgac cgagggcaca acagccgtcg aaactaagct attcgtcgta gcggacaaag
     7381 tccgacagat acacactgac aactcgacat tgttcaacag agtccacaag ttaaagtaca
     7441 agatcaacga aacaaaatga ccaaagtgga caagataatc cacaatgtac gacaagtaga
     7501 caatgtaaca gctagacaag taccacttgt cgaaatttac gtggtttttg agcattttcg
     7561 agactacata gatagaaaaa atgtggcaaa agtagacacg tatacctgtc aaaagggaaa
     7621 ctatagattg ccacttgtca acaagatgaa aacaaacaat cagaactacg aagtgactat
     7681 ctatgttctc ggtattcttg gctcagatcc ttccgtattt agccagtatg ttctctagtg
     7741 tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca
     7801 tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt
     7861 gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt
     7921 tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat
     7981 ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt
     8041 tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc
     8101 ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa
     8161 tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa
     8221 tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt
     8281 tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc
     8341 ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc
     8401 tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat
     8461 tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt
     8521 ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct
     8581 tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa
     8641 ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac caattgagat
     8701 ggg
//

