LOCUS       pLPT145                 2491 bp    DNA     linear       11-NOV-2016
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="pZE21-GFPaav"
                     /mol_type="other DNA"
     CDS             109..151
                     /label=ORF frame 1
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRPAANDENYAAAV"
     terminator      437..480
                     /label=rrnB_T1_terminator
                     /translation="IKRKAQSKDWAFRF"
     rep_origin      complement(669..1288)
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA.SAACKQKNHRYQRWFVCRIKSYQLFFRR.L
                     ASAERRYQILSF.CSRS.ATTSRTL.HRLHTSLC.SCYQWLLPVAISRVLPGWTQDDS
                     YRIRRSGRAERGVRAHSPAWSERPTPN.DTYSVSIEKAPRFPKGERRTGIR.AAGSEQ
                     ESARGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     promoter        6..59
                     /note="PltetO1"
     promoter        25..30
                     /note="-35"
     promoter        6..24
                     /note="tetO2"
     promoter        31..49
                     /note="tetO2"
     promoter        48..53
                     /note="-10"
     CDS             complement(1485..2279)
                     /label=ORF frame 3
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(1488..2267)
                     /dnas_title="NeoR/KanR"
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="DGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLF
                     VKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSH
                     LAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2368..2415)
                     /label=NEOKAN_promoter
                     /translation="GIASWGALW.GWEALQ"
     source          283..375
                     /organism="repressilator (pZS1-lTlrLLtCL)"
                     /mol_type="other DNA"
     promoter        complement(161..209)
                     /note="pR"
     promoter        complement(194..209)
                     /note="OR2"
     promoter        complement(191..196)
                     /note="-35"
     promoter        complement(171..186)
                     /note="OR1"
     promoter        complement(170..175)
                     /note="-10"
     promoter        383..388
                     /note="-35"
     promoter        383..405
                     /note="pL Lac O1"
     promoter        389..405
                     /note="LacO"
     source          406..3476
                     /dnas_title="pLPT145"
                     /organism="repressilator (pZS1-lTlrLLtCL)"
                     /mol_type="other DNA"
     source          complement(161..282)
                     /dnas_title="pZS1-lTlrLLtCL"
                     /organism="repressilator (pZS1-lTlrLLtCL)"
                     /mol_type="other DNA"
     promoter        248..264
                     /note="LacO"
     promoter        248..264
                     /note="pL Lac O1"
     source          265..646
                     /dnas_title="pZS1-lTlrLLtCL"
                     /organism="repressilator (pZS1-lTlrLLtCL)"
                     /mol_type="other DNA"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccgcaa ggcctgcagc
      121 aaacgacgaa aactacgctg catcagttta ataagcttga gcaaccatta tcaccgccag
      181 aggtaaaata gtcaacacgc acggtgttag atatttatcc AAGGAACGCC AGCCGCCACG
      241 ACGTTTGttg tgagcggata acaaGTGGAA TGTCTTTTGT GAcccgaaaa gtgccacctg
      301 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      361 cctttcgtct tcacctcgag aattgacatt gtgagcggat aacaagatcc catggtacgc
      421 gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      481 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggc
      541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1321 aactcacgtt aagggatttt ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc
     1381 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1441 tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca
     1501 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     1561 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     1621 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     1681 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     1741 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     1801 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     1861 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     1921 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     1981 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     2041 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     2101 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     2161 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     2221 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     2281 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt
     2341 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     2401 ccagctggca attccgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2461 aggcgtatca cgaggccctt tcgtcttcac c
//

