LOCUS       pZE21-GFPaav	2982 bp 	DNA	SYN
DEFINITION  pZE21-GFPaav
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2286
                     /dnas_title="pLPT148"
                     /organism="pZE21-GFPaav"
                     /mol_type="other DNA"
     CDS             109..151
                     /label=ORF frame 1
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRPAANDENYAAAV"
     terminator      232..275
                     /label=rrnB_T1_terminator
                     /translation="IKRKAQSKDWAFRF"
     rep_origin      complement(464..1083)
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA.SAACKQKNHRYQRWFVCRIKSYQLFFRR.L
                     ASAERRYQILSF.CSRS.ATTSRTL.HRLHTSLC.SCYQWLLPVAISRVLPGWTQDDS
                     YRIRRSGRAERGVRAHSPAWSERPTPN.DTYSVSIEKAPRFPKGERRTGIR.AAGSEQ
                     ESARGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     CDS             complement(1280..2074)
                     /label=ORF frame 3
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(1283..2062)
                     /dnas_title="NeoR/KanR"
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="DGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLF
                     VKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSH
                     LAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2163..2210)
                     /label=NEOKAN_promoter
                     /translation="GIASWGALW.GWEALQ"
     promoter        6..59
                     /note="PltetO1"
     promoter        25..30
                     /note="-35"
     promoter        6..24
                     /note="tetO2"
     promoter        31..49
                     /note="tetO2"
     promoter        48..53
                     /note="-10"
     promoter        184..200
                     /note="LacO"
     promoter        161..177
                     /note="LacO"
     promoter        178..183
                     /note="-35"
     promoter        161..200
                     /note="pL Lac O1"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccgcaa ggcctgcagc
      121 aaacgacgaa aactacgctg catcagttta ataagcttga ttgtgagcgg ataacaattg
      181 acattgtgag cggataacaa gatcccatgg tacgcgtgct agaggcatca aataaaacga
      241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      301 ctgagtagga caaatccgcc gccctagacc taggcgttcg gctgcggcga gcggtatcag
      361 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      421 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      481 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      541 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      601 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      661 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      721 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      781 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      841 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      901 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      961 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1141 tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1201 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1261 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     1321 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     1381 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     1441 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     1501 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     1561 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     1621 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     1681 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     1741 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     1801 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     1861 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     1921 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1981 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     2041 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     2101 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     2161 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc gacgtctaag
     2221 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2281 ttcacc
//

