LOCUS       Exported               11031 bp ds-DNA     circular SYN 01-JUN-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CMV-pTACSLIK-YAP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Eric
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 11, 2016 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        86..389
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             680..1426
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /note="rtTA-Advanced"
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1855..1871)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1879..1895
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1903..1933)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1948..1969
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2257..2845)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        complement(3879..3983)
                     /gene="bla"
                     /note="AmpR promoter"
     primer_bind     4457..4473
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4952..5760
                     /note="ApoE.HCR.hAAT"
     misc_feature    5787..6073
                     /note="synthetic intron"
     misc_feature    6085..8069
                     /note="CreER"
     CDS             8091..8144
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8166..8876
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     protein_bind    8944..8964
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        8999..9313
                     /note="tight TRE promoter"
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9007..9025
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9043..9061
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9078..9096
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9114..9132
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9150..9168
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9185..9203
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9221..9239
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    9321..11017
                     /note="x2 Flag YAP"
     CDS             9357..9380
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             9414..9437
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
ORIGIN
        1 tgataacatc tcgagccata aagatggtta attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gggcgcgccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
      661 ttctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa acggagctct
      721 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
      781 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
      841 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
      901 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
      961 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     1021 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     1081 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     1141 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     1201 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     1261 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     1321 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     1381 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaagctcga
     1441 gcaaatttgg atccccttga aatacatcca caggtacacc tccaattgac tcaaatgatg
     1501 tcaattagtc tatcagaagc ttctaaagcc atgacatcat tttctggaat tttccaagct
     1561 gtttaaaggc acagtcaact tagtgtatgt aaacttctga cccactggaa ttgtgataca
     1621 gtgaattata agtgaaataa tctgtctgta aacaattgtt ggaaaaatga cttgtgtcat
     1681 gcacaaagta gatgtcctaa ctgacttgcc aaaactattg tttgttaaca agaaatttgt
     1741 ggagtagttg aaaaacgagt tttaatgact ccaacttaag tgtatgtaaa cttccgactt
     1801 caactgtata ggtctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
     1861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3361 tggtatggct tcattcagct ccggttccca acgatcaagg gcgagttaca tgatccccca
     3421 tgttgtgcaa aaaagcggtt agctcctttc ggtcctccga tcgttgtcag aagtaagttg
     3481 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3541 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3601 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3661 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3721 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3781 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3841 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3901 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3961 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     4021 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     4081 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4141 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4201 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4261 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     4321 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     4381 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4441 tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac cctacagttg
     4501 aagtcggaag tttacataca cttaagttgg agtcattaaa actcgttttt caactactcc
     4561 acaaatttct tgttaacaaa caatagtttt ggcaagtcag ttaggacatc tactttgtgc
     4621 atgacacaag tcatttttcc aacaattgtt tacagacaga ttatttcact tataattcac
     4681 tgtatcacaa ttccagtggg tcagaagttt acatacacta agttgactgt gcctttaaac
     4741 agcttggaaa attccagaaa atgatgtcat ggctttagaa gcttctgata gactaattga
     4801 catcatttga gtcaattgga ggtgtacctg tggatgtatt tcaaggaatt ctgtggaatg
     4861 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4921 tgcatccctt tcgcgcgcga aaccctccgg agttaggctc agaggcacac aggagtttct
     4981 gggctcaccc tgcccccttc caacccctca gttcccatcc tccagcagct gtttgtgtgc
     5041 tgcctctgaa gtccacactg aacaaacttc agcctactca tgtccctaaa atgggcaaac
     5101 attgcaagca gcaaacagca aacacacagc cctccctgcc tgctgacctt ggagctgggg
     5161 cagaggtcag agacctctct gggcccatgc cacctccaac atccactcga ccccttggaa
     5221 tttcggtgga gaggagcaga ggttgtcctg gcgtggttta ggtagtgtga gaggggtacc
     5281 cggggatctt gctaccagtg gaacagccac taaggattct gcagtgagag cagagggcca
     5341 gctaagtggt actctcccag agactgtctg actcacgcca ccccctccac cttggacaca
     5401 ggacgctgtg gtttctgagc caggtacaat gactcctttc ggtaagtgca gtggaagctg
     5461 tacactgccc aggcaaagcg tccgggcagc gtaggcgggc gactcagatc ccagccagtg
     5521 gacttagccc ctgtttgctc ctccgataac tggggtgacc ttggttaata ttcaccagca
     5581 gcctcccccg ttgcccctct ggatccactg cttaaatacg gacgaggaca gggccctgtc
     5641 tcctcagctt caggcaccac cactgacctg ggacagtgaa tgatccccct gatctgcggc
     5701 ctcgacggta tcgataagct tgatatcgaa ttctagtcgt cgaccacttt cacaatctgc
     5761 tagcaacctg aggaggttat cgtacgaaat tcgctgtctg cgagggccag ctgttggggt
     5821 gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac
     5881 gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc
     5941 tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatcgat
     6001 ctgaccatac acttgagtga caatgacatc cactttgcct ttctctccac aggtgtccac
     6061 tcccaggtcc aaccttaatt aagcggacca tgtccaattt actgaccgta caccaaaatt
     6121 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
     6181 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
     6241 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
     6301 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
     6361 atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca
     6421 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
     6481 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
     6541 atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc
     6601 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
     6661 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
     6721 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
     6781 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
     6841 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
     6901 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
     6961 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
     7021 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
     7081 aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct gctggagaca
     7141 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
     7201 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
     7261 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
     7321 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
     7381 gctttgtgga tttgaccctc catgatcagg tccaccttct tgaatgtgcc tggctagaga
     7441 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc
     7501 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
     7561 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
     7621 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
     7681 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
     7741 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
     7801 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
     7861 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag gcggcggacg
     7921 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
     7981 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
     8041 gggaggcaga gggtttccct gccacagctg ctacgcgtga aggcagtgga gagggcagag
     8101 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaagacga atcgccggcg
     8161 tatggatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     8221 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     8281 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     8341 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     8401 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     8461 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     8521 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     8581 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     8641 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     8701 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     8761 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     8821 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtagatgc
     8881 gcatgaattc gtttaaacag ggacagcaga gatccagttt ggttaattct agacagttat
     8941 caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatcccga
     9001 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     9061 aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     9121 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     9181 tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     9241 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     9301 ccgtcagatc gccactagtg agctcgttta gtgaccgtca gaattgatct accatggact
     9361 acaaagacga tgacgacaag cttgcggccg cgaattcaag cttagccacc atggactaca
     9421 aagacgatga cgataaagca aggctcgaat cggtacctaa ggatcccggg cagcagccgc
     9481 cgcctcaacc ggccccccag ggccaagggc agccgccttc gcagcccccg caggggcagg
     9541 gcccgccgtc cggacccggg caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac
     9601 cccccgccgg gcatcagatc gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc
     9661 tcttcaacgc cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc
     9721 tccggaagct gcccgactcc ttcttcaagc cgccggagcc caaatcccac tcccgacagg
     9781 ccagtactga tgcaggcact gcaggagccc tgactccaca gcatgttcga gctcattcct
     9841 ctccagcttc tctgcagttg ggagctgttt ctcctgggac actgaccccc actggagtag
     9901 tctctggccc agcagctaca cccacagctc agcatcttcg acagtcttct tttgagatac
     9961 ctgatgatgt acctctgcca gcaggttggg agatggcaaa gacatcttct ggtcagagat
    10021 acttcttaaa tcacatcgat cagacaacaa catggcagga ccccaggaag gccatgctgt
    10081 cccagatgaa cgtcacagcc cccaccagtc caccagtgca gcagaatatg atgaactcgg
    10141 cttcaggtcc tcttcctgat ggatgggaac aagccatgac tcaggatgga gaaatttact
    10201 atataaacca taagaacaag accacctctt ggctagaccc aaggcttgac cctcgttttg
    10261 ccatgaacca gagaatcagt cagagtgctc cagtgaaaca gccaccaccc ctggctcccc
    10321 agagcccaca gggaggcgtc atgggtggca gcaactccaa ccagcagcaa cagatgcgac
    10381 tgcagcaact gcagatggag aaggagaggc tgcggctgaa acagcaagaa ctgcttcggc
    10441 aggagttagc cctgcgtagc cagttaccaa cactggagca ggatggtggg actcaaaatc
    10501 cagtgtcttc tcccgggatg tctcaggaat tgagaacaat gacgaccaat agctcagatc
    10561 ctttccttaa cagtggcacc tatcactctc gagatgagag tacagacagt ggactaagca
    10621 tgagcagcta cagtgtccct cgaaccccag atgacttcct gaacagtgtg gatgagatgg
    10681 atacaggtga tactatcaac caaagcaccc tgccctcaca gcagaaccgt ttcccagact
    10741 accttgaagc cattcctggg acaaatgtgg accttggaac actggaagga gatggaatga
    10801 acatagaagg agaggagctg atgccaagtc tgcaggaagc tttgagttct gacatcctta
    10861 atgacatgga gtctgttttg gctgccacca agctagataa agaaagcttt cttacatggt
    10921 tatagagccc tcaggcagac tgaattctgc agatatccag cacagtggcg gccgctcgag
    10981 tctagaggat cccgggtggc atccctgtga cccctccgtc gagatatcta g
//
