LOCUS       Exported               11602 bp ds-DNA     circular SYN 11-NOV-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pTC-ApoE-Tet-YAP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 11, 2016 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   415..768
                     /note="IR/DRs"
     misc_feature    842..873
                     /note="mcs"
     promoter        874..1682
                     /note="ApoE.HCR.hAAT"
     intron          1709..1995
                     /note="synthetic intron"
     gene            2007..3991
                     /note="CreER"
     misc_feature    4004..4066
                     /note="2A peptide"
     exon            4088..4795
                     /note="mCherry"
     misc_feature    4796..4798
                     /note="stop"
     repeat_region   4865..4890
                     /note="attB1"
     primer_bind     5206..5225
                     /note="pCEP fwd"
                     /note="112 - pCEP fwd"
     misc_feature    5243..6939
                     /note="x2-Flag-YAP"
     repeat_region   6954..6978
                     /note="attB2"
     promoter        7014..7822
                     /note="ApoE.HCR.hAAT"
     intron          7849..8135
                     /note="synthetic intron"
     CDS             8204..8950
                     /note="rtTA3"
     repeat_region   8979..9332
                     /note="IR/DRs"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accctacagt
      421 tgaagtcgga agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact
      481 ccacaaattt cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt
      541 gcatgacaca agtcattttt ccaacaattg tttacagaca gattatttca cttataattc
      601 actgtatcac aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa
      661 acagcttgga aaattccaga aaatgatgtc atggctttag aagcttctga tagactaatt
      721 gacatcattt gagtcaattg gaggtgtacc tgtggatgta tttcaaggaa ttctgtggaa
      781 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      841 catgcatccc tttcgcgcgc gaaaccctcc ggagttaggc tcagaggcac acaggagttt
      901 ctgggctcac cctgccccct tccaacccct cagttcccat cctccagcag ctgtttgtgt
      961 gctgcctctg aagtccacac tgaacaaact tcagcctact catgtcccta aaatgggcaa
     1021 acattgcaag cagcaaacag caaacacaca gccctccctg cctgctgacc ttggagctgg
     1081 ggcagaggtc agagacctct ctgggcccat gccacctcca acatccactc gaccccttgg
     1141 aatttcggtg gagaggagca gaggttgtcc tggcgtggtt taggtagtgt gagaggggta
     1201 cccggggatc ttgctaccag tggaacagcc actaaggatt ctgcagtgag agcagagggc
     1261 cagctaagtg gtactctccc agagactgtc tgactcacgc caccccctcc accttggaca
     1321 caggacgctg tggtttctga gccaggtaca atgactcctt tcggtaagtg cagtggaagc
     1381 tgtacactgc ccaggcaaag cgtccgggca gcgtaggcgg gcgactcaga tcccagccag
     1441 tggacttagc ccctgtttgc tcctccgata actggggtga ccttggttaa tattcaccag
     1501 cagcctcccc cgttgcccct ctggatccac tgcttaaata cggacgagga cagggccctg
     1561 tctcctcagc ttcaggcacc accactgacc tgggacagtg aatgatcccc ctgatctgcg
     1621 gcctcgacgg tatcgataag cttgatatcg aattctagtc gtcgaccact ttcacaatct
     1681 gctagcaacc tgaggaggtt atcgtacgaa attcgctgtc tgcgagggcc agctgttggg
     1741 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
     1801 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
     1861 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatcg
     1921 atctgaccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc
     1981 actcccaggt ccaaccttaa ttaagcggac catgtccaat ttactgaccg tacaccaaaa
     2041 tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat
     2101 gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg
     2161 gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga
     2221 tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca
     2281 acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag
     2341 caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg
     2401 tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga
     2461 aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac
     2521 cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg
     2581 tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga
     2641 gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt
     2701 agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc
     2761 atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg
     2821 attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag
     2881 tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat
     2941 gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag
     3001 tgaaacaggg gcaatggtgc gcctgctgga agatggcgat ctcgagccat ctgctggaga
     3061 catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag
     3121 cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc
     3181 catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt
     3241 actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc
     3301 aggctttgtg gatttgaccc tccatgatca ggtccacctt cttgaatgtg cctggctaga
     3361 gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc
     3421 tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt
     3481 cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt
     3541 tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac
     3601 cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac
     3661 tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc
     3721 ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct
     3781 gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga
     3841 cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca
     3901 aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac
     3961 gggggaggca gagggtttcc ctgccacagc tgctacgcgt gaaggcagtg gagagggcag
     4021 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaagac gaatcgccgg
     4081 cgtatggatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     4141 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     4201 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     4261 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     4321 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     4381 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4441 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4501 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4561 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4621 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4681 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4741 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtagat
     4801 gcgcatgaat tcgtttaaac agggacagca gagatccagt ttggttaatt ctagacagtt
     4861 atcaacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccc
     4921 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     4981 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     5041 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     5101 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     5161 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     5221 aaccgtcaga tcgccactag tgagctcgtt tagtgaccgt cagaattgat ctaccatgga
     5281 ctacaaagac gatgacgaca agcttgcggc cgcgaattca agcttagcca ccatggacta
     5341 caaagacgat gacgataaag caaggctcga atcggtacct aaggatcccg ggcagcagcc
     5401 gccgcctcaa ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca
     5461 gggcccgccg tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc
     5521 accccccgcc gggcatcaga tcgtgcacgt ccgcggggac tcggagaccg acctggaggc
     5581 gctcttcaac gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag
     5641 gctccggaag ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca
     5701 ggccagtact gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcattc
     5761 ctctccagct tctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt
     5821 agtctctggc ccagcagcta cacccacagc tcagcatctt cgacagtctt cttttgagat
     5881 acctgatgat gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag
     5941 atacttctta aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct
     6001 gtcccagatg aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc
     6061 ggcttcaggt cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta
     6121 ctatataaac cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt
     6181 tgccatgaac cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc
     6241 ccagagccca cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg
     6301 actgcagcaa ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg
     6361 gcaggagtta gccctgcgta gccagttacc aacactggag caggatggtg ggactcaaaa
     6421 tccagtgtct tctcccggga tgtctcagga attgagaaca atgacgacca atagctcaga
     6481 tcctttcctt aacagtggca cctatcactc tcgagatgag agtacagaca gtggactaag
     6541 catgagcagc tacagtgtcc ctcgaacccc agatgacttc ctgaacagtg tggatgagat
     6601 ggatacaggt gatactatca accaaagcac cctgccctca cagcagaacc gtttcccaga
     6661 ctaccttgaa gccattcctg ggacaaatgt ggaccttgga acactggaag gagatggaat
     6721 gaacatagaa ggagaggagc tgatgccaag tctgcaggaa gctttgagtt ctgacatcct
     6781 taatgacatg gagtctgttt tggctgccac caagctagat aaagaaagct ttcttacatg
     6841 gttatagagc cctcaggcag actgaattct gcagatatcc agcacagtgg cggccgctcg
     6901 agtctagagg atcccgggtg gcatccctgt gacccctccg tcgagatatc tagacccagc
     6961 tttcttgtac aaagtggttg ataacatctc gagccataaa gatggttaat taagttaggc
     7021 tcagaggcac acaggagttt ctgggctcac cctgccccct tccaacccct cagttcccat
     7081 cctccagcag ctgtttgtgt gctgcctctg aagtccacac tgaacaaact tcagcctact
     7141 catgtcccta aaatgggcaa acattgcaag cagcaaacag caaacacaca gccctccctg
     7201 cctgctgacc ttggagctgg ggcagaggtc agagacctct ctgggcccat gccacctcca
     7261 acatccactc gaccccttgg aatttcggtg gagaggagca gaggttgtcc tggcgtggtt
     7321 taggtagtgt gagaggggta cccggggatc ttgctaccag tggaacagcc actaaggatt
     7381 ctgcagtgag agcagagggc cagctaagtg gtactctccc agagactgtc tgactcacgc
     7441 caccccctcc accttggaca caggacgctg tggtttctga gccaggtaca atgactcctt
     7501 tcggtaagtg cagtggaagc tgtacactgc ccaggcaaag cgtccgggca gcgtaggcgg
     7561 gcgactcaga tcccagccag tggacttagc ccctgtttgc tcctccgata actggggtga
     7621 ccttggttaa tattcaccag cagcctcccc cgttgcccct ctggatccac tgcttaaata
     7681 cggacgagga cagggccctg tctcctcagc ttcaggcacc accactgacc tgggacagtg
     7741 aatgatcccc ctgatctgcg gcctcgacgg tatcgataag cttgatatcg aattctagtc
     7801 gtcgaccact ttcacaatct gctagcaacc tgaggaggtt atcgtacgaa attcgctgtc
     7861 tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga cttctgcgct
     7921 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc
     7981 tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt caagcttgag
     8041 gtgtggcagg cttgagatcg atctgaccat acacttgagt gacaatgaca tccactttgc
     8101 ctttctctcc acaggtgtcc actcccaggt ccaacggcgc gccgctagcg ctaccggact
     8161 cagatctcga gctcaagctt cgaattctgc agtcgaattc accatgtcta gactggacaa
     8221 gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac
     8281 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     8341 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     8401 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     8461 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     8521 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     8581 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     8641 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     8701 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     8761 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     8821 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     8881 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     8941 ccccgggtaa ctaagtaagc tcgagcaaat ttggatcccc ttgaaataca tccacaggta
     9001 cacctccaat tgactcaaat gatgtcaatt agtctatcag aagcttctaa agccatgaca
     9061 tcattttctg gaattttcca agctgtttaa aggcacagtc aacttagtgt atgtaaactt
     9121 ctgacccact ggaattgtga tacagtgaat tataagtgaa ataatctgtc tgtaaacaat
     9181 tgttggaaaa atgacttgtg tcatgcacaa agtagatgtc ctaactgact tgccaaaact
     9241 attgtttgtt aacaagaaat ttgtggagta gttgaaaaac gagttttaat gactccaact
     9301 taagtgtatg taaacttccg acttcaactg tataggtcta gagtcgacct gcaggcatgc
     9361 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9421 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9481 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9541 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9601 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10921 aagggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc tttcggtcct
    10981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11521 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    11581 cgtatcacga ggccctttcg tc
//
