LOCUS       pPEPY-lacI-GmR          5925 bp    DNA     circular SYN 12-NOV-2016
DEFINITION  Ligation of inverted Cut pPEPY into cut lacI-GmR
ACCESSION   pPEPY-lacI-GmR
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             1..356
                     /gene="'prsA"
                     /codon_start=3
     misc_feature    4..504
                     /gene="ISU#"
                     /product="Integration Site Up"
     misc_signal     362..387
                     /label=TER
     misc_signal     513..572
                     /label=PF6 ->
                     /product="PF6 promoter (-52 to +8)"
     CDS             601..1689
                     /gene="lacI"
                     /product="LacI repressor (E. coli)"
     CDS             1713..2252
                     /gene="GmR"
                     /product="Gentamicin Resistance (Gentamicin
                     3'-acetyltransferase)"
     misc_signal     2005
                     /label=g\a
     misc_signal     2274..2313
                     /label=T_B1006 ->|<-
                     /product="Terminator: B1006 (modified) (synthetic;
                     bidirectional)"
     misc_feature    2344..2763
                     /gene="ISD#"
                     /product="Integration Site Down"
     misc_signal     2356..2405
                     /label=TER
     CDS             complement(2409..2642)
                     /gene="hypoth."
     misc_feature    complement(3644..4302)
                     /gene="'oUC18"
                     /product="pUC18 origin of replication (multi copy)"
     misc_signal     complement(4881..4906)
                     /label=T_KanR
                     /product="Terminator: KanR"
     CDS             complement(4944..5735)
                     /gene="kan"
     misc_signal     complement(5736..5824)
                     /label=P_PF6
                     /product="Promoter: PF6"
ORIGIN      
        1 ctagtgaagt tatgaacatc atcggtaagg ttgaaggcaa gacttgtatc ttgattgatg
       61 atatgattga taccgctgga acgatttgtc atgcggcaga tgctcttgcg gaagctggtg
      121 ctgttgaagt ctatgcaagc tgtacgcacc cagttctttc tggtcctgct acggacaata
      181 tccaaaaatc agctattaag aaattggttg ttttggatac catctatctg ccagaagagc
      241 gtttgattga taagattgag cagatttcaa tcgctcatct cctaggggat gctatcgtac
      301 gtattcatga aaaacgacca ctttctccac ttttcgatat tgagaaaaaa atttaatgac
      361 caagcctgag atgattctca ggtttttcgt cttttttccg aataaataga tagtagcggt
      421 gaatctagta aagctagatt taaaactgtg atataataaa aagagaagaa atatgactgt
      481 acgtcatcag aagtttcagc gaccctagat cctgctcgaa gatttcagct tgacattgca
      541 ctgtccccct ggtataataa ctatacatgc aaggatctaa ataaataagg aggaaaatta
      601 atgaaaccag tcacccttta cgatgtcgca gaatacgcag gagtctctta ccaaacagtc
      661 agtcgtgtag tcaatcaggc tagtcatgta tcagcaaaaa ctcgtgaaaa ggttgaagct
      721 gcaatggctg aacttaatta catcccaaat cgtgttgccc aacagttggc tggtaaacaa
      781 agtttgctta tcggagttgc tacctcttca ttggcacttc atgccccttc tcaaattgtt
      841 gccgctatca aatctcgtgc agatcaattg ggtgcaagtg ttgtcgtatc tatggttgaa
      901 cgtagtggag tcgaagcctg taaggcagcc gtccacaatt tattggctca acgtgtatct
      961 ggtttaatta tcaattaccc attggatgac caagatgcca ttgctgtcga agctgcatgt
     1021 accaatgtac cagcattatt tttggatgtt tcagatcaaa cacctatcaa ttcaatcatc
     1081 tttagtcatg aagatggtac tcgtttggga gtcgaacatt tggtagctct tggtcaccaa
     1141 cagattgctc ttttagcagg accacttagt tctgtttcag cccgtcttcg tttagctggt
     1201 tggcacaaat acttaactcg taatcaaatt cagcctatcg ctgaacgtga gggtgattgg
     1261 tctgcaatgt caggatttca acagacaatg caaatgttga atgaaggtat tgtaccaact
     1321 gcaatgttgg ttgccaatga ccaaatggcc cttggagcta tgcgtgcaat taccgaatca
     1381 ggtttgcgtg ttggagctga tattagtgtt gtcggttacg atgatacaga agattcaagt
     1441 tgttacattc cacctttgac aactatcaag caagattttc gtttgcttgg acagacaagt
     1501 gttgatcgtt tattgcaact ttctcagggt caggctgtca agggaaatca gcttttacct
     1561 gtatctcttg ttaagcgtaa gaccacatta gcaccaaata ctcaaaccgc ctcacctcgt
     1621 gccttggcag atagtcttat gcagcttgca cgtcaggtct ctcgtttgga aagtggacaa
     1681 taataataag gatctaaata ggaggaaaat taatgttacg cagcagcaac gatgttacgc
     1741 agcagggcag tcgccctaaa acaaagttag gtggctcaag tatgggcatc attcgcacat
     1801 gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg
     1861 agttcggaga cgtagccacc tactcccaac atcagccgga ctccgattac ctcgggaact
     1921 tgctccgtag taagacattc atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg
     1981 ctctcgcggc ttacgttctg cccaagtttg agcagccgcg tagtgaaatc tatatctatg
     2041 atctcgcagt ctccggcgag caccggaggc agggcattgc caccgcgctc atcaatctcc
     2101 tcaagcatga ggccaacgcg cttggtgctt atgtgatcta cgtgcaagca gattacggtg
     2161 acgatcccgc agtggctctc tatacaaagt tgggcatacg ggaagaagtg atgcactttg
     2221 atatcgaccc aagtaccgcc acctaataat aaggatccct ccagtaactc gagaaaaaaa
     2281 aaccgcgccc ctgacagggc gcggtttttt tttaagtgga cacctacgta tggtaactgt
     2341 acactacaca aaataggctc cataatatcc ataggggatt tactcactat ggatattatg
     2401 gagccatttt acaacagtgc ctcaaattca gatactgaca tacctaattg ctggctggca
     2461 acctcagaag tcagaagacc ttggcgtact atgtctagga aggtaaaaag tttcctttct
     2521 gcccttcctt caactttccc acgctctagt ccttgttcaa ggtcttcagc cctagcagtt
     2581 tccaaggcat agtcctgtgc taacaagact tgttcttctc gcatatgtag ttgactaaac
     2641 attttcctgt cctcctcgga ccagctcttg tagtccagca gttgatttgc ttgggtaatg
     2701 gctcgttgcg gttgctgggt aaagggcttg ttgccgaaaa actccaacca tggcttgcga
     2761 accttgtctt tgctggtttc tctgtatttt ttaattccaa gaatgccatc ttaaccagat
     2821 ggttttcctg tccattgttg gtaatcgcca atacctcacc tgttgtgtct tcgcgcatac
     2881 taaagctatg aaaagccagg tcatctgaga aataattact atccacgata gcaatgacgt
     2941 aaacaggagc gatgtgtttg tagatctaaa taaataagga ggaaaattaa tgccggatcc
     3001 ctccagtaac tcgagaaagt gtaaagttga gcagtgtctt tagtaaattt agggacttgt
     3061 tttgtcatag ttttgatacc tgtattcgtt gtttcatgag catttgacgt ttcttttcat
     3121 gtaggcattt caacaattag aatgatattt ttccgaggat gaacttatat attgtgactt
     3181 tttagagtta tgatgcctct tgatttgact atcgctatca atttcatcca cttttacagt
     3241 ttcatggttt tatgaaccga aacatctagg tacaggtttt agcatcaatt gattgttttt
     3301 gagccatatg aagttgaaac gtggagatac ttttcttgtg ataacgccct attcaaagag
     3361 gaataaccgt ttttggagag agttcagtac tatgtggcgc taaatactat atgtaaaaca
     3421 tatattaaac aagatttaac taaggagaat tgtaaaaaca aaaatgagaa tttcatataa
     3481 agtttattct aggagtatgt atcttgaata aaaattgtta tttaagttct gggggttatg
     3541 atgaatgttt ttatgcggcc gcgggataac gcaggaaaga acatgtgagc aaaaggcata
     3601 aaaacattca tcataacccc cagaacttaa ataacgcggc cgcgggataa cgcaggaaag
     3661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4141 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4261 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4321 ttgatctttt ctacggggtg gtaccaaacc ttactaaagt atataattta ggcagtcaat
     4381 tctgtatgat aatgtttgta ctatcgtcga gtttttgctt gattgcctaa cgagataaat
     4441 tttagaagag ttttcggtgt gatttgacaa gtttctgact ggaaggatag ttacactagt
     4501 attgtttatt aaaatgagca gatagagaaa atttggaatc tatttgctta ttatcaatcg
     4561 tttttttagc ttatttgtct tagattggta tagaactgag ctatggatta atcttcttct
     4621 atcttatcct gttcctaaat atgttggtag aaaaatgaag cagttgttac gagtgtctgt
     4681 attggaaaaa aattttgtct atacaattct ttaaatgata tgaagactag gaatgccaaa
     4741 acactgacat atttcgttta aagcctatcc agtctcttca attgtatttg tagaaatgct
     4801 atcagtaata tattcgcgaa cactatgatt tacaacagtg tggtcgtaca tctttgtaac
     4861 tttattatac agcttctagc aaaaaactgg acggagtccc gtccagaaaa gtaagcactt
     4921 gttattgcaa taaaattagc ctaaaacaat tcatccagta aaatataata ttttattttc
     4981 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
     5041 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
     5101 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
     5161 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
     5221 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
     5281 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
     5341 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
     5401 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
     5461 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
     5521 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
     5581 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
     5641 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
     5701 tttttcaatt ccggtgatat tctcatttta gccattaatt ttcctcctta tttatttaga
     5761 tccttgcatg tatagttatt ataccagggg gacagtgcaa tgtcaagctg aaatcttcga
     5821 gcagaagaac cttgccaagc aagattcttt tgatagggtg gtctccgatc gtgtactgca
     5881 gaaaactagt cgcaaaaaac cccgcttcgg cggggttttt tcgct
//
