LOCUS       pPEPX1-P3-sgRNAs        3415 bp    DNA     circular SYN 28-MAR-2015
DEFINITION  
ACCESSION   pPEPX1-P3-sgRNAs
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  Fragment 2
               Fragment ends: XhoI and EcoRI
               Fragment size: 3188
            Insert molecule:  Fragment 2
               Fragment ends: EcoRI and XhoI
               Fragment size: 227
FEATURES             Location/Qualifiers
     misc_signal     10..61
                     /label=T_rpsI ->|
                     /product="Terminator: S. pneumoniae rpsI (forward)"
                     /SECDrawAs="Label"
     misc_signal     complement(66..93)
                     /label=T_tuf |<-
                     /product="Terminator: S. pneumoniae tuf (reverse)"
                     /SECDrawAs="Label"
     misc_feature    106..324
                     /gene="MCS''"
                     /product="Multiple Cloning Site (BglFusion)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     137..170
                     /label=T_B1002 ->|<-
                     /product="Terminator: B1002 (synthetic; bidirectional)"
                     /SECDrawAs="Label"
     misc_signal     195..323
                     /label=T_B0015 ->|->|
                     /product="Terminator: B0015 (E. coli rrnBT1 + T7TE; double
                     forward)"
                     /SECDrawAs="Label"
     misc_feature    343..396
                     /gene="P3"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     361..366
                     /label=-35
                     /SECDrawAs="Label"
     misc_signal     384..389
                     /label=-10
                     /SECDrawAs="Label"
     misc_signal     396
                     /label=+1
                     /SECDrawAs="Label"
     misc_signal     397..415
                     /label=base-pairing luc
                     /SECDrawAs="Label"
     misc_feature    397..508
                     /gene="sgRNA"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     416..462
                     /label=dCas9 handle
                     /SECDrawAs="Label"
     misc_signal     468..508
                     /label=ter S. pyo
                     /SECDrawAs="Label"
     misc_signal     509..535
                     /label=from Qi vector
                     /SECDrawAs="Label"
     misc_feature    552..556
                     /gene="'MCS"
                     /product="Multiple Cloning Site (BglFusion)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     557..596
                     /label=T_B1006 ->|<-
                     /product="Terminator: B1006 (modified) (synthetic;
                     bidirectional)"
                     /SECDrawAs="Label"
     misc_feature    627..1119
                     /gene="ISD"
                     /product="Integration Site Down (S. pneumoniae D39
                     1678908-1679400)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1160..1855
                     /gene="oUC18"
                     /product="pUC18 origin of replication (multi copy)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1864..2339
                     /gene="ISU"
                     /product="Integration Site Up (S. pneumoniae D39
                     1678030-1678505)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     complement(2360..2385)
                     /label=T_SpR |<-
                     /product="Terminator: SpR"
                     /SECDrawAs="Label"
     CDS             complement(2420..3202)
                     /gene="SpR"
                     /product="Spectinomycin Resistance (Streptomycin
                     3''-adenylyltransferase)"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     complement(3357..3391)
                     /label=P_SpR <-
                     /product="Promoter: SpR (-35 to -1) (L. lactis P32)"
                     /SECDrawAs="Label"
ORIGIN      
        1 gcatgcggca aagcactcaa aagtttacct tatgggtgct tttttcgtgc ttttttgaaa
       61 agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat agggtggtct ccgatcgtgt
      121 actgcagaaa actagtcgca aaaaaccccg cttcggcggg gttttttcgc tctagacggt
      181 gatcaacacg ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      241 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      301 gggtgggcct ttctgcgttt atagaattcg gtcgacagat ctccattcta cagtttattc
      361 ttgacattgc actgtccccc tggtataata actatataga ggatagaatg gcgccgttta
      421 agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
      481 aaagtggcac cgagtcggtg ctttttttga agcttgggcc cgaacaaaaa ctcatggatc
      541 cctccagtaa ctcgagaaaa aaaaaccgcg cccctgacag ggcgcggttt ttttttaagt
      601 ggacacctac gtatggtaac tgtacaggtc gctgaaactt ctatcgtcaa gaagaatcac
      661 caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa gatttctatg
      721 actgatattg cccatcagct ttccatctca acttcaactg ttattcgtaa gctcaatgat
      781 tttcacttta aacatgattt ttcgcgtctt cctgagatta tgtcttggga tgagtatgct
      841 tttacaaagg gaaagatgag cttcattgcg caagatttta acaatctcaa tatcatcact
      901 gttcttgagg gaagaacaca agctatcatt cgaaatcact ttcttcgcta cgatagagtt
      961 gtccgatgtc gcgtcaaaat tattactatg gatatgttta gcccttacta tgacttggct
     1021 aaacagcttc gctttcaaat ttctaggctc aggctgaaac agtctcccag actgtttcac
     1081 tcccgaatgc taaaatcgtt cttgatcgct ttcacattgg acgtcctgac catggagtca
     1141 agtgtaaggc actggtacca ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1201 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1261 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1321 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1381 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1441 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1501 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1561 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1621 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1681 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1741 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1801 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccgcggc
     1861 cgcagttaaa gaacttggac tgcgctctaa aaccaacgtg gtcagtctgg acaagattat
     1921 tattgataaa aaatcctcac tgataaccgg tttcccagag tttcggatgg tttggaaggt
     1981 ggtccgccag cgtgtggtgg atgatctggt atccgttctg gatacggact atctggatat
     2041 ggaactaatc ccaaatctca ctcgccaaat cgctgagcag tctatctatt cttatataga
     2101 aaatggcctc aaactcctta ttgattatgc tcagaaggaa atcaccattg accactcaag
     2161 cgaccgagac aagattctca tggacattgg caaagaccct tatgtcgttt cgattaaatc
     2221 aaaagtctat ctccaagacg gacgccaatt tcagtttacc gaaagtcgcc ataagttaga
     2281 aaaatttaga tttgtagatt ttgcaaaacg caagaaataa aagactgaga caccagatca
     2341 tggctagaaa gcttctagca aaaaactgga cggagtcccg tccagaaaag taagcacttg
     2401 ttattgcaat aaaattagcc taattgagag aagtttctat agaatttttc atatacttaa
     2461 cgagtgcttt cacctttgaa tatagtcctt cccacttatc atcacactct ccccgatagc
     2521 cttttctagc tatatccagt aaagttacat gctctttagg taaaagaggt atagcccatt
     2581 cagcagcgac atctttcgag gtaatttcac cagtagtcac tgtttgccac attcgagcta
     2641 gggttaaaat tacattacgc tcatcacctt ttatcccctc aattagttct ggcaaagaat
     2701 ccttaattgc tcttcgaata tctgtcaaag gtacggagac aagtatactt gaagaatcag
     2761 gaccaaatag agaaatacta ttctttcttg cttgtgctaa aacaatagcc aaatcaggat
     2821 catagcttgg ttcctgaatt tgtccattct caaattcacc cctgagccac tcaccgtata
     2881 taaattctct ttttggagga tattgccaag ggacaacttc actcctattt ataaccgtaa
     2941 cttcaagtgg tctaacagaa tccgtatttc caatccttcc tgatatagtc attagtcttt
     3001 ctgttagttt ttttcgagtt aattgaggta aactatgatt cacgacgact agaacatcta
     3061 catcgctgtt aatgcgtaaa ccaccattta ctgctgaacc aaatagatat actccaacta
     3121 ttgaacttcc aaataaatct tttacgattt ttaatgtttg aatcgcttga tttggtattt
     3181 ttccgttaat caaattgctc atgatttcac ctcgttgatt atgttcatat aaagtttata
     3241 ttgatactca atttacttac cctagattgg acatatactt aaattactgt tcaataaagc
     3301 tgaccgttag cgtttaagta catcctttca caatttgtct acagaacagt actggcctac
     3361 ctagtatagc attttgtgaa gtttttttct agtccaagct cacaaaaatc caaag
//
