LOCUS       Exported                4352 bp ds-DNA     circular SYN 15-NOV-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p433-pCSDest-ACR1-Nm
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Amrani
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 29, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    97..108
                     /note="kozak"
     CDS             106..360
                     /codon_start=1
                     /note="Acr1_Nm"
                     /translation="MNKTYKIGKNAGYDGCGLCLAAISENEAIKVKYLRDICPDYDGDD
                     KAEDWLRWGTDSRVKAAALEMEQYAYTSVGMASCWEFVEL"
     promoter        complement(379..396)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    401..535
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(648..666)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(687..703)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    711..727
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(735..765)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    780..801
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1089..1677)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1848..2708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2709..2813)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(2839..3294)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3368..4352
                     /note="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaagccg ccaccatgaa taaaacttat
      121 aaaattggaa aaaatgccgg gtatgatggc tgcggtcttt gtcttgcggc catttctgaa
      181 aatgaagcta tcaaagttaa gtatttgcgc gacatttgtc ctgattacga tggcgatgat
      241 aaagctgagg attggctgag atggggaacg gacagccgcg tcaaagcagc cgctcttgaa
      301 atggagcagt acgcatatac gtcggttggt atggcctcat gttgggagtt tgttgaacta
      361 tgactcgagc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
      421 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
      481 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
      541 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
      601 cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg
      661 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1021 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1081 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1261 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1441 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1501 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1561 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1681 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1741 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2701 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2761 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2821 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     2881 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2941 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3001 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3061 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3121 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3181 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3241 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     3301 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     3361 gccagtcgac catagccaat tcaatatggc gtatatggac tcatgccaat tcaatatggt
     3421 ggatctggac ctgtgccaat tcaatatggc gtatatggac tcgtgccaat tcaatatggt
     3481 ggatctggac cccagccaat tcaatatggc ggacttggca ccatgccaat tcaatatggc
     3541 ggacttggca ctgtgccaac tggggagggg tctacttggc acggtgccaa gtttgaggag
     3601 gggtcttggc cctgtgccaa gtccgccata ttgaattggc atggtgccaa taatggcggc
     3661 catattggct atatgccagg atcaatatat aggcaatatc caatatggcc ctatgccaat
     3721 atggctattg gccaggttca atactatgta ttggccctat gccatatagt attccatata
     3781 tgggttttcc tattgacgta gatagcccct cccaatgggc ggtcccatat accatatatg
     3841 gggcttccta ataccgccca tagccactcc cccattgacg tcaatggtct ctatatatgg
     3901 tctttcctat tgacgtcata tgggcggtcc tattgacgta tatggcgcct cccccattga
     3961 cgtcaattac ggtaaatggc ccgcctggct caatgcccat tgacgtcaat aggaccaccc
     4021 accattgacg tcaatgggat ggctcattgc ccattcatat ccgttctcac gccccctatt
     4081 gacgtcaatg acggtaaatg gcccacttgg cagtacatca atatctatta atagtaactt
     4141 ggcaagtaca ttactattgg aaggacgcca gggtacattg gcagtactcc cattgacgtc
     4201 aatggcggta aatggcccgc gatggctgcc aagtacatcc ccattgacgt caatggggag
     4261 gggcaatgac gcaaatgggc gttccattga cgtaaatggg cggtaggcgt gcctaatggg
     4321 aggtctatat aagcaatgct cgtttaggga ac
//
