LOCUS       pKIR1_1                18518 bp ds-DNA     circular     17-NOV-2016
DEFINITION  Cloning vector pKIR1_1 DNA, complete sequence.
ACCESSION   LC189564
VERSION     LC189564.[1]
KEYWORDS    .
SOURCE      Cloning vector pKIR1_1
  ORGANISM  Cloning vector pKIR1_1 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2016) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroki Tsutsui Nagoya University, Graduate School of
            Science; 1 Furo-cho, Nagoya, Aichi 464-8602, Japan
REFERENCE   2  
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene
            knockout in Arabidopsis thaliana
  JOURNAL   Plant Cell Physiol. (2016) In press
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..18518
                     /db_xref="taxon:1907936"
                     /mol_type="other DNA"
                     /organism="Cloning vector pKIR1_1"
                     /label=source:Cloning vector pKIR1_1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      76..1739
                     /note="AtRPS5A promoter"
                     /regulatory_class="promoter"
                     /label=AtRPS5A promoter
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=#66ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1742..1810
                     /note="3xFLAG"
                     /label=3xFLAG
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1811..1861
                     /note="NLS"
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1862..5962
                     /note="human-codon-optimized SpCas9"
                     /label=human-codon-optimized SpCas9
                     /ApEinfo_fwdcolor=#ffff66
                     /ApEinfo_revcolor=#ffff66
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5963..6010
                     /note="nucleoplasmin NLS peptide"
                     /label=nucleoplasmin NLS peptide
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6011..6013
                     /note="stop codon"
                     /label=stop codon
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      6023..6272
                     /note="Heat_shock_protein18.2 terminator"
                     /regulatory_class="terminator"
                     /label=Heat_shock_protein18.2 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6318..9398
                     /note="OLE1-TagRFP cassette"
                     /label=OLE1-TagRFP cassette
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      9418..9860
                     /note="AtU6.26 promoter"
                     /regulatory_class="promoter"
                     /label=AtU6.26 promoter
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=#80ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9861..9861
                     /note="initial G for transcription"
                     /label=initial G for transcription
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(9866..9872)
                     /note="AarI recognition site"
                     /label=AarI recognition site
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9879..9885
                     /note="AarI recognition site"
                     /label=AarI recognition site(1)
                     /ApEinfo_label=AarI recognition site
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9890..9965
                     /note="sgRNA_scaffold"
                     /label=sgRNA_scaffold
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      9966..9976
                     /regulatory_class="terminator"
                     /label=regulatory
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10065..10089
                     /note="Right Border"
                     /label=Right Border
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    16600..16625
                     /note="Left Border"
                     /label=Left Border
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cgacgtcgca tgctcccggc cgccatggcc gcgggatatc acaagtttgt acaaaaaagc
       61 aggctccgcg gccgcgatcc ctcaactttt gattcgctat ttgcagtgca cctgtggcgt
      121 tcatcacatc ttttgtgaca ctgtttgcac tggtcattgc tattacaaag gaccttcctg
      181 atgttgaagg agatcgaaag taagtaactg cacgcataac cattttcttt ccgctctttg
      241 gctcaatcca tttgacagtc aaagacaatg tttaaccagc tccgtttgat atattgtctt
      301 tatgtgtttg ttcaagcatg tttagttaat catgcctttg attgatcttg aataggttcc
      361 aaatatcaac cctggcaaca aaacttggag tgagaaacat tgcattcctc ggttctggac
      421 ttctgctagt aaattatgtt tcagccatat cactagcttt ctacatgcct caggtgaatt
      481 catctatttc cgtcttaact atttcggtta attaaagcac gaacaccatt actgcatgta
      541 gaagcttgat aaactatcgc caccaattta tttttgttgc gatattgtta ctttcctcag
      601 tatgcagctt tgaaaagacc aaccctctta tcctttaaca atgaacaggt ttttagaggt
      661 agcttgatga ttcctgcaca tgtgatcttg gcttcaggct taattttcca ggtaaagcat
      721 tatgagatac tcttatatct cttacatact tttgagataa tgcacaagaa cttcataact
      781 atatgcttta gtttctgcat ttgacactgc caaattcatt aatctctaat atctttgttg
      841 ttgatctttg gtagacatgg gtactagaaa aagcaaacta caccaaggta aaatactttt
      901 gtacaaacat aaactcgtta tcacggaaca tcaatggagt gtatatctaa cggagtgtag
      961 aaacatttga ttattgcagg aagctatctc aggatattat cggtttatat ggaatctctt
     1021 ctacgcagag tatctgttat tccccttcct ctagctttca atttcatggt gaggatatgc
     1081 agttttcttt gtatatcatt cttcttcttc tttgtagctt ggagtcaaaa tcggttcctt
     1141 catgtacata catcaaggat atgtccttct gaatttttat atcttgcaat aaaaatgctt
     1201 gtaccaattg aaacaccagc tttttgagtt ctatgatcac tgacttggtt ctaaccaaaa
     1261 aaaaaaaatg tttaatttac atatctaaaa gtaggtttag ggaaacctaa acagtaaaat
     1321 atttgtatat tattcgaatt tcactcatca taaaaactta aattgcacca taaaattttg
     1381 ttttactatt aatgatgtaa tttgtgtaac ttaagataaa aataatattc cgtaagttaa
     1441 ccggctaaaa ccacgtataa accagggaac ctgttaaacc ggttctttac tggataaaga
     1501 aatgaaagcc catgtagaca gctccattag agcccaaacc ctaaatttct catctatata
     1561 aaaggagtga cattagggtt tttgttcgtc ctcttaaagc ttctcgtttt ctctgccgtc
     1621 tctctcattc gcgcgacgca aacgatcttc aggtgatctt ctttctccaa atcctctctc
     1681 ataactctga tttcgtactt gtgtatttga gctcacgctc tgtttctctc accacagccc
     1741 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
     1801 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
     1861 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
     1921 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     1981 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     2041 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     2101 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     2161 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     2221 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     2281 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     2341 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     2401 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     2461 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     2521 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     2581 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     2641 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     2701 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     2761 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     2821 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     2881 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     2941 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     3001 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     3061 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     3121 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     3181 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     3241 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     3301 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     3361 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     3421 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     3481 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     3541 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     3601 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     3661 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     3721 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     3781 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     3841 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     3901 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     3961 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     4021 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     4081 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     4141 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     4201 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     4261 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     4321 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     4381 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     4441 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     4501 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     4561 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     4621 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     4681 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     4741 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     4801 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     4861 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     4921 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     4981 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     5041 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     5101 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     5161 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     5221 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     5281 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     5341 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     5401 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     5461 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     5521 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     5581 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     5641 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     5701 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     5761 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     5821 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     5881 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     5941 cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     6001 aaagaaaaag taaaagggtg ggatatgaag atgaagatga aatatttggt gtgtcaaata
     6061 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     6121 ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat
     6181 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     6241 ggtatggact atggaatatg attaaagata aggcgcgccg acccagcttt cttgtacaaa
     6301 gtggtgatat cactagtgta tgtaggtata gtaacatgaa caagaacaga ctcaagtaca
     6361 agatcgcata cgaaaatgga aatggcaatg tcacttccac ataatcaaac acgaatcctc
     6421 atatcaacaa ggcctgagat tctaactagc tcataacaac ttagccaata gttacttgag
     6481 actaccaaat gtatgtagaa ctaaagacta agggacagag agttcgtcta aacaggtgaa
     6541 tctagtcgtt gttatctaat aaacaattca gccccaaatg cagaacacac atagagctct
     6601 ctattgattc aaattacgat ctgatactga taacgtctag atttttaggg ttaaagcaat
     6661 caatcacctg acgattcaag gtggttggat catgacgatt ccagaaaaca tcaagcaagc
     6721 tctcaaagct acactctttg ggatcatact gaactctaac aacctcgtta tgtcccgtag
     6781 tgccagtaca gacatcctcg taactcggat tgtgcacgat gccatggcta tacccaacct
     6841 cggtcttggt cacaccagga actctctggt aagctagctc cactccccag aaacaaccgg
     6901 cgccaaattg cgcgaattgc tgacctgaag acggaacatc atcgtcgggt ccttgggcga
     6961 ttgcggcgga agatgggtca gcttgggctt gaggacgaga cccgaatccg agtctgttga
     7021 aaaggttgtt cattggggat ttgtatacgg agattggtcg tcgagaggtt tgagggaaag
     7081 gacaaatggg tttggctctg gagaaagaga gtgcggcttt agagagagaa ttgagaggtt
     7141 tagagagaga tgcggcggcg atgagcggag gagagacgac gaggacctgc attatcaaag
     7201 cagtgacgtg gtgaaatttg gaacttttaa gaggcagata gatttattat ttgtatccat
     7261 tttcttcatt gttctagaat gtcgcggaac aaattttaaa actaaatcct aaatttttct
     7321 aattttgttg ccaatagtgg atatgtgggc cgtatagaag gaatctattg aaggcccaaa
     7381 cccatactga cgagcccaaa ggttcgtttt gcgttttatg tttcggttcg atgccaacgc
     7441 cacattctga gctaggcaaa aaacaaacgt gtctttgaat agactcctct cgttaacaca
     7501 tgcagcggct gcatggtgac gccattaaca cgtggcctac aattgcatga tgtctccatt
     7561 gacacgtgac ttctcgtctc ctttcttaat atatctaaca aacactccta cctcttccaa
     7621 aatatataca catctttttg atcaatctct cattcaaaat ctcattctct ctagtaaaca
     7681 agaacaaaaa aatggcggat acagctagag gaacccatca cgatatcatc ggcagagacc
     7741 agtacccgat gatgggccga gaccgagacc agtaccagat gtccggacga ggatctgact
     7801 actccaagtc taggcagatt gctaaagctg caactgctgt cacagctggt ggttccctcc
     7861 ttgttctctc cagccttacc cttgttggaa ctgtcatagc tttgactgtt gcaacacctc
     7921 tgctcgttat cttcagccca atccttgtcc cggctctcat cacagttgca ctcctcatca
     7981 ccggttttct ttcctctgga gggtttggca ttgccgctat aaccgttttc tcttggattt
     8041 acaagtaagc acacatttat catcttactt cataattttg tgcaatatgt gcatgcatgt
     8101 gttgagccag tagctttgga tcaatttttt tggtcgaata acaaatgtaa caataagaaa
     8161 ttgcaaattc tagggaacat ttggttaact aaatacgaaa tttgacctag ctagcttgaa
     8221 tgtgtctgtg tatatcatct atataggtaa aatgcttggt atgataccta ttgattgtga
     8281 ataggtacgc aacgggagag cacccacagg gatcagacaa gttggacagt gcaaggatga
     8341 agttgggaag caaagctcag gatctgaaag acagagctca gtactacgga cagcaacata
     8401 ctggtgggga acatgaccgt gaccgtactc gtggtggcca gcacactact atgagcgagc
     8461 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     8521 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     8581 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     8641 acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     8701 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     8761 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     8821 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca
     8881 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     8941 tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg
     9001 ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg
     9061 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     9121 tccctagcaa actggggcac aagtgaatcg ttcaaacatt tggcaataaa gtttcttaag
     9181 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     9241 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     9301 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     9361 taaattatcg cgcgcggtgt catctatgtt actagatcac tagtgcggcc gcctgcatcg
     9421 ttgaacaacg gaaactcgac ttgccttccg cacaatacat catttcttct tagctttttt
     9481 tcttcttctt cgttcataca gttttttttt gtttatcagc ttacattttc ttgaaccgta
     9541 gctttcgttt tcttcttttt aactttccat tcggagtttt tgtatcttgt ttcatagttt
     9601 gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa tttgattgaa taaaacatct
     9661 tcattcttaa gatatgaaga taatcttcaa aaggcccctg ggaatctgaa agaagagaag
     9721 caggcccatt tatatgggaa agaacaatag tatttcttat ataggcccat ttaagttgaa
     9781 aacaatcttc aaaagtccca catcgcttag ataagaaaac gaagctgagt ttatatacag
     9841 ctagagtcga agtagtgatt gaaatgcagg tgatgactca cctgcataag ttttagagct
     9901 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     9961 ggtgcttttt tttttttgca ggtcgaccat atgggagagc tcaagcttag cttgagcttg
    10021 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
    10081 cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa
    10141 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
    10201 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
    10261 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
    10321 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
    10381 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
    10441 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
    10501 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
    10561 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
    10621 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
    10681 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
    10741 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
    10801 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
    10861 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
    10921 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
    10981 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
    11041 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
    11101 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
    11161 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
    11221 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
    11281 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
    11341 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
    11401 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
    11461 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
    11521 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
    11581 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
    11641 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
    11701 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
    11761 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
    11821 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
    11881 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
    11941 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
    12001 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
    12061 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
    12121 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
    12181 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
    12241 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
    12301 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
    12361 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
    12421 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat
    12481 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
    12541 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
    12601 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
    12661 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
    12721 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
    12781 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
    12841 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
    12901 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
    12961 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
    13021 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
    13081 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
    13141 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
    13201 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
    13261 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
    13321 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
    13381 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
    13441 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
    13501 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
    13561 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
    13621 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
    13681 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
    13741 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
    13801 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
    13861 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
    13921 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
    13981 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    14041 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    14101 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
    14161 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    14221 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
    14281 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    14341 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    14401 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    14461 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    14521 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    14581 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    14641 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    14701 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    14761 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    14821 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    14881 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    14941 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    15001 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    15061 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
    15121 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
    15181 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taatcgcttg agttaacgcc
    15241 ggcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg
    15301 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
    15361 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
    15421 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
    15481 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
    15541 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
    15601 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
    15661 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
    15721 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
    15781 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
    15841 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
    15901 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
    15961 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
    16021 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta
    16081 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
    16141 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
    16201 aaaaacatgt cataacaaga agccatgaaa accgccactg cgccgttacc accgctgcgt
    16261 tcggtcaagg ttctggacca gttgcgtgac ggcagttacg ctacttgcat tacagcttac
    16321 gaaccgaacg aggcttatgt ccactgggtt cgtgcccgaa ttgatcacag gcagcaacgc
    16381 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
    16441 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
    16501 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
    16561 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
    16621 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat
    16681 taacgccgaa ttgaattatc agcttgcatg ccggtcgatc tagtaacata gatgacaccg
    16741 cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt
    16801 ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt
    16861 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
    16921 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct gcttgactct
    16981 agggaattaa ttcctgaatc actgcgaccg gccctcccgc gacccagccg agcgagctta
    17041 gcgaactgtg gacgagaact gtgccaccaa gcgtaaggcc gttctctcgc atttgccttg
    17101 ctaggctcgc gcgagttgct ggctgaggcg ttctcgaaat cagctcttgt tcggtcggca
    17161 tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
    17221 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
    17281 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
    17341 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
    17401 cgcccggagc cttggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
    17461 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
    17521 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
    17581 gacattgttg gagccgaatc cgcgtgcacg agtgccggac ttcggggcag tcctcgccca
    17641 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
    17701 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
    17761 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
    17821 agcgatagca tccatagcct ccgcgaccgg ctgaagaaca gcgggcagtt cggtttcagg
    17881 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
    17941 gaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
    18001 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
    18061 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
    18121 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
    18181 ctttttcata ggggggattc gagttgagag tgaatatgag actctaattg gataccgagg
    18241 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
    18301 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
    18361 ctccagaaac ccgcggctca gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
    18421 aaaacggctt gtcccgcgtc atcggcgggg tcataacgtg actcccttaa ttctcatgta
    18481 tgataattcg agggtacccg gggatcctct agagggcc  
//
