LOCUS       Exported               11319 bp ds-DNA     circular SYN 28-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAVS1-Neo-UBC-M2rtTA-H2BmNeon
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     [DEFINITION]:  - [LOCUS]: pCAGGS-M2rtTA-AAV-neo
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    33..833
                     /note="Homology Arm-Left"
     misc_feature    877..948
                     /note="T2A"
     misc_feature    961..1764
                     /note="Neo-R"
     misc_feature    2333..3543
                     /note="hUBC promoter"
     misc_feature    3565..4337
                     /note="rtTA-M2"
     misc_feature    complement(5052..5762)
                     /note="mNeon"
     misc_feature    complement(5763..6158)
                     /note="H2B"
     misc_feature    complement(6165..6503)
                     /note="tetO"
     misc_feature    6551..7390
                     /note="Homology Arm-Right"
     misc_feature    complement(9307..9966)
                     /note="Amp-R"
     misc_feature    10266..10894
                     /note="ColE1 origin"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagacgcgt
      961 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1021 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1081 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1141 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1201 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1261 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1321 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1381 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1441 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1501 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1561 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1621 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1681 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1741 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct
     1801 attaaacaat aaagatgtcc actaaaagga agtttttctg cagaaattga tgatctatta
     1861 aacaataaag atgtccacta aaatgaagtt ttttactgtc atactttgtt aagaagggtg
     1921 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
     1981 ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt
     2041 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
     2101 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
     2161 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
     2221 agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa
     2281 ttccatcaga agctgggagc tccaccgcgg tggcggccgc tctagaacta gtgcctccgc
     2341 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     2401 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     2461 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     2521 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     2581 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     2641 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     2701 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     2761 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     2821 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tccggggagc gcagcaaaat
     2881 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     2941 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     3001 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     3061 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     3121 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     3181 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     3241 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     3301 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     3361 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     3421 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     3481 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     3541 gaccccgggc tgcaggaatt caccatgcca aagagaccca gaccctctag actggacaag
     3601 agcaaagtca taaacggcgc tctggaatta ctcaatggag tcggtatcga aggcctgacg
     3661 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     3721 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     3781 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc
     3841 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     3901 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     3961 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     4021 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     4081 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     4141 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     4201 gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     4261 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     4321 cccgggtaac taagtaagga tccagacatg ataagataca ttgatgagtt tggacaaacc
     4381 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4441 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4501 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     4561 ggtatggctg attatgatcc tgcaagcctc gtcgtcctgg ccggaccacg ctatctgtgc
     4621 aaggtccccg gccccggacg cgcgctccat gagcagagcg cccgccgccg aggcgaagac
     4681 tcgggcggcg ccctgcccgt cccaccaggt caacaggcgg taaccggcct cttcatcggg
     4741 aatgcgcgcg accttcagca tcgccggcat gtccccctgg cggacgggaa gtatccagct
     4801 cgaccaagct tagtactgtc gaggcagtga aaaaaatgct ttatttgtga aatttgtgat
     4861 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4921 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     4981 ctctacaaat gtggtatggc tgattatgat cctctagaga tatcgtcgac aagcttatcg
     5041 atgcggccgc tttacttgta cagctcgtcc atgcccatca catcggtaaa ggccttttgc
     5101 cactccttga agttgagctc ggtcttggag tgcttgagct ccgtcttacg gaacacgtac
     5161 atcggctggt tcttcagata gttagccgcc attggcttgg caaaggtgta ggtggtccgc
     5221 gcagtgctcc ggtagcgctt gccatttcca gtggtgtaac tccacttaaa ggtactgatg
     5281 atggttttgt cgttggggta agtcttcttc gacctgcacc agtccgcagc ggtcagcgag
     5341 ttggtcatca caggaccgtc agcagggaaa ccagtcccct tcacctgggc ctctcctttg
     5401 atgtggcttc cctcgtaggt gtagcggtag ttaacagtaa gggaggcacc atcttcaaac
     5461 tgcattgtgc gatggacttg gtatccggag ccatctacca tggcggcctg gaaaggcgac
     5521 atcccgtcag ggtagggcag gtactgatgg aagccatacc cgatatgagg gaccagaatc
     5581 cagggggaga actggaggtc acccttggtg gacttcaggt ttaactcctc ataaccatca
     5641 tttggattgc cggtgccctg acccaccatg tcaaagtcca caccgttgat ggagccaaag
     5701 atgtgtaact catgtgtcgc tgggagagag gccatgttat cctcctcgcc cttgctcacc
     5761 atggtggcga ccggtggatc cttagcgctg gtgtacttgg tgatggcctt agtaccctcg
     5821 gacacggcgt gcttggccaa ctccccaggc agcagcaggc gcacggccgt ctggatctcc
     5881 ctggaggtga tggtcgagcg cttgttgtaa tgcgccaggc gggaagcctc acctgcgatg
     5941 cgctcgaaaa tgtcgttcac aaacgaattc atgatgccca tggccttgga cgaaatgccg
     6001 gtgtcagggt ggacctgctt cagaaccttg tacacataga tggaatagct ctccttgcgg
     6061 ctgcgcttgc gcttcttgcc gcctttcttc tgcgccttag tcaccgcctt cttggagccc
     6121 tttttcgggg cgggagcaga cttcgctggc tctggcatgg tggcggtacc gagctcgaat
     6181 tctccaggcg atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac
     6241 acgcctacct cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc
     6301 tatcactgat agggataaac tcgacatacg ttctctatca ctgataggga gtaaactcga
     6361 catacgttct ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg
     6421 gagtaaactc gacatcgttc tctatcactg atagggagta aactcgacat acgttctcta
     6481 tcactgatag ggagtaaact cgacccatag agcccaccgc atccccagca tgcctgctat
     6541 taagctttac tagggacagg attggtgaca gaaaagcccc atccttaggc ctcctccttc
     6601 ctagtctcct gatattgggt ctaaccccca cctcctgtta ggcagattcc ttatctggtg
     6661 acacaccccc atttcctgga gccatctctc tccttgccag aacctctaag gtttgcttac
     6721 gatggagcca gagaggatcc tgggagggag agcttggcag ggggtgggag ggaagggggg
     6781 gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct accctctccc agaacctgag
     6841 ctgctctgac gcggctgtct ggtgcgtttc actgatcctg gtgctgcagc ttccttacac
     6901 ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa taagttggtc ctgagttcta
     6961 actttggctc ttcacctttc tagtccccaa tttatattgt tcctccgtgc gtcagtttta
     7021 cctgtgagat aaggccagta gccagccccg tcctggcagg gctgtggtga ggaggggggt
     7081 gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg
     7141 ccgcctctac tccctttctc tttctccatc cttctttcct taaagagtcc ccagtgctat
     7201 ctgggacata ttcctccgcc cagagcaggg tcccgcttcc ctaaggccct gctctgggct
     7261 tctgggtttg agtccttggc aagcccagga gaggcgctca ggcttccctg tcccccttcc
     7321 tcgtccacca tctcatgccc ctggctctcc tgccccttcc ctacaggggt tcctggctct
     7381 gctctaaggg caagggcgaa ttcgcggccg ctaaattcaa ttcgccctat agtgagtcgt
     7441 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7501 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7561 gcaccgatcg cccttcccaa cagttgcgca gcctatacgt acggcagttt aaggtttaca
     7621 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     7681 cgccggggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     7741 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     7801 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     7861 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggcatga
     7921 gattatcaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac
     7981 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
     8041 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
     8101 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
     8161 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
     8221 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     8281 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     8341 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     8401 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     8461 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     8521 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     8581 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     8641 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     8701 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     8761 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     8821 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8881 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8941 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     9001 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt
     9061 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9121 cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9181 aatacattca aatatgtatc cgctcatgag attatcaaaa aggatcttca cctagatcct
     9241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10201 gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    11101 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    11161 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    11221 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    11281 tgattacgcc aagctcagaa ttaaccctca ctaaaggga
//
