LOCUS       pKI1_1R                18522 bp ds-DNA     circular     23-NOV-2016
DEFINITION  Cloning vector pKI1_1R DNA, complete sequence.
ACCESSION   LC189566
VERSION     LC189566.1
KEYWORDS    .
SOURCE      Cloning vector pKI1_1R
  ORGANISM  Cloning vector pKI1_1R other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2016) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroki Tsutsui Nagoya University, Graduate School of
            Science; 1 Furo-cho, Nagoya, Aichi 464-8602, Japan
REFERENCE   2
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene
            knockout in Arabidopsis thaliana
  JOURNAL   Plant Cell Physiol. (2016) In press
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..18522
                     /db_xref="taxon:1907938"
                     /mol_type="other DNA"
                     /organism="Cloning vector pKI1_1R"
                     /label=source:Cloning vector pKI1_1R
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      76..518
                     /note="AtU6.26 promoter"
                     /regulatory_class="promoter"
                     /label=AtU6.26 promoter
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=#80ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    519..519
                     /note="initial G for transcription"
                     /label=initial G for transcription
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(524..530)
                     /note="AarI recognition site"
                     /label=AarI recognition site
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    537..543
                     /note="AarI recognition site"
                     /label=AarI recognition site(1)
                     /ApEinfo_label=AarI recognition site
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    548..623
                     /note="sgRNA_scaffold"
                     /label=sgRNA_scaffold
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      624..634
                     /regulatory_class="terminator"
                     /label=regulatory
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      643..2306
                     /note="AtRPS5A promoter"
                     /regulatory_class="promoter"
                     /label=AtRPS5A promoter
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=#66ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2312..2377
                     /note="3xFLAG"
                     /label=3xFLAG
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2378..2428
                     /note="NLS"
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2429..6529
                     /note="human-codon-optimized SpCas9"
                     /label=human-codon-optimized SpCas9
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6530..6577
                     /note="nucleoplasmin NLS peptide"
                     /label=nucleoplasmin NLS peptide
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6578..6580
                     /note="stop codon"
                     /label=stop codon
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      6590..6839
                     /note="Heat_shock_protein18.2 terminator"
                     /regulatory_class="terminator"
                     /label=Heat_shock_protein18.2 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6885..9965
                     /note="OLE1-TagRFP cassette"
                     /label=OLE1-TagRFP cassette
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9890..9965
                     /note="sgRNA_scaffold"
                     /label=sgRNA_scaffold(1)
                     /ApEinfo_label=sgRNA_scaffold
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    10065..10089
                     /note="Right Border"
                     /label=Right Border
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    16604..16629
                     /note="Left Border"
                     /label=Left Border
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cgacgtcgca tgctcccggc cgccatggcc gcgggatatc acaagtttgt acaaaaaagc
       61 aggctccgcg gccgctcgtt gaacaacgga aactcgactt gccttccgca caatacatca
      121 tttcttctta gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt
      181 acattttctt gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg
      241 tatcttgttt catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt
      301 tgattgaata aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg
      361 aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat
      421 aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga
      481 agctgagttt atatacagct agagtcgaag tagtgattga aatgcaggtg atgactcacc
      541 tgcataagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      601 aaaaagtggc accgagtcgg tgcttttttt ttttgcggcc gcgatccctc aacttttgat
      661 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtgacactg tttgcactgg
      721 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
      781 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
      841 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
      901 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
      961 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgtttca gccatatcac
     1021 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
     1081 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
     1141 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
     1201 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
     1261 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
     1321 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
     1381 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
     1441 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
     1501 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
     1561 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     1621 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     1681 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     1741 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     1801 tgatcactga cttggttcta accaaaaaaa aaaaatgttt aatttacata tctaaaagta
     1861 ggtttaggga aacctaaaca gtaaaatatt tgtatattat tcgaatttca ctcatcataa
     1921 aaacttaaat tgcaccataa aattttgttt tactattaat gatgtaattt gtgtaactta
     1981 agataaaaat aatattccgt aagttaaccg gctaaaacca cgtataaacc agggaacctg
     2041 ttaaaccggt tctttactgg ataaagaaat gaaagcccat gtagacagct ccattagagc
     2101 ccaaacccta aatttctcat ctatataaaa ggagtgacat tagggttttt gttcgtcctc
     2161 ttaaagcttc tcgttttctc tgccgtctct ctcattcgcg cgacgcaaac gatcttcagg
     2221 tgatcttctt tctccaaatc ctctctcata actctgattt cgtacttgtg tatttgagct
     2281 cacgctctgt ttctctcacc acagccccat ggactataag gaccacgacg gagactacaa
     2341 ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa
     2401 ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg
     2461 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     2521 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     2581 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     2641 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     2701 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     2761 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     2821 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     2881 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     2941 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     3001 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     3061 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     3121 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     3181 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     3241 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     3301 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     3361 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     3421 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     3481 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     3541 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     3601 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     3661 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     3721 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     3781 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     3841 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     3901 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     3961 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     4021 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     4081 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     4141 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     4201 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     4261 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     4321 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     4381 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     4441 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     4501 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     4561 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     4621 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     4681 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     4741 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     4801 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     4861 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     4921 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     4981 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     5041 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     5101 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     5161 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     5221 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     5281 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     5341 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     5401 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     5461 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     5521 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     5581 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     5641 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     5701 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     5761 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     5821 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     5881 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     5941 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     6001 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     6061 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     6121 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     6181 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     6241 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     6301 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     6361 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     6421 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     6481 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca aaaggccggc
     6541 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagtaa aagggtggga tatgaagatg
     6601 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     6661 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatctca
     6721 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     6781 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagg
     6841 cgcgccgacc cagctttctt gtacaaagtg gtgatatcac tagtgtatgt aggtatagta
     6901 acatgaacaa gaacagactc aagtacaaga tcgcatacga aaatggaaat ggcaatgtca
     6961 cttccacata atcaaacacg aatcctcata tcaacaaggc ctgagattct aactagctca
     7021 taacaactta gccaatagtt acttgagact accaaatgta tgtagaacta aagactaagg
     7081 gacagagagt tcgtctaaac aggtgaatct agtcgttgtt atctaataaa caattcagcc
     7141 ccaaatgcag aacacacata gagctctcta ttgattcaaa ttacgatctg atactgataa
     7201 cgtctagatt tttagggtta aagcaatcaa tcacctgacg attcaaggtg gttggatcat
     7261 gacgattcca gaaaacatca agcaagctct caaagctaca ctctttggga tcatactgaa
     7321 ctctaacaac ctcgttatgt cccgtagtgc cagtacagac atcctcgtaa ctcggattgt
     7381 gcacgatgcc atggctatac ccaacctcgg tcttggtcac accaggaact ctctggtaag
     7441 ctagctccac tccccagaaa caaccggcgc caaattgcgc gaattgctga cctgaagacg
     7501 gaacatcatc gtcgggtcct tgggcgattg cggcggaaga tgggtcagct tgggcttgag
     7561 gacgagaccc gaatccgagt ctgttgaaaa ggttgttcat tggggatttg tatacggaga
     7621 ttggtcgtcg agaggtttga gggaaaggac aaatgggttt ggctctggag aaagagagtg
     7681 cggctttaga gagagaattg agaggtttag agagagatgc ggcggcgatg agcggaggag
     7741 agacgacgag gacctgcatt atcaaagcag tgacgtggtg aaatttggaa cttttaagag
     7801 gcagatagat ttattatttg tatccatttt cttcattgtt ctagaatgtc gcggaacaaa
     7861 ttttaaaact aaatcctaaa tttttctaat tttgttgcca atagtggata tgtgggccgt
     7921 atagaaggaa tctattgaag gcccaaaccc atactgacga gcccaaaggt tcgttttgcg
     7981 ttttatgttt cggttcgatg ccaacgccac attctgagct aggcaaaaaa caaacgtgtc
     8041 tttgaataga ctcctctcgt taacacatgc agcggctgca tggtgacgcc attaacacgt
     8101 ggcctacaat tgcatgatgt ctccattgac acgtgacttc tcgtctcctt tcttaatata
     8161 tctaacaaac actcctacct cttccaaaat atatacacat ctttttgatc aatctctcat
     8221 tcaaaatctc attctctcta gtaaacaaga acaaaaaaat ggcggataca gctagaggaa
     8281 cccatcacga tatcatcggc agagaccagt acccgatgat gggccgagac cgagaccagt
     8341 accagatgtc cggacgagga tctgactact ccaagtctag gcagattgct aaagctgcaa
     8401 ctgctgtcac agctggtggt tccctccttg ttctctccag ccttaccctt gttggaactg
     8461 tcatagcttt gactgttgca acacctctgc tcgttatctt cagcccaatc cttgtcccgg
     8521 ctctcatcac agttgcactc ctcatcaccg gttttctttc ctctggaggg tttggcattg
     8581 ccgctataac cgttttctct tggatttaca agtaagcaca catttatcat cttacttcat
     8641 aattttgtgc aatatgtgca tgcatgtgtt gagccagtag ctttggatca atttttttgg
     8701 tcgaataaca aatgtaacaa taagaaattg caaattctag ggaacatttg gttaactaaa
     8761 tacgaaattt gacctagcta gcttgaatgt gtctgtgtat atcatctata taggtaaaat
     8821 gcttggtatg atacctattg attgtgaata ggtacgcaac gggagagcac ccacagggat
     8881 cagacaagtt ggacagtgca aggatgaagt tgggaagcaa agctcaggat ctgaaagaca
     8941 gagctcagta ctacggacag caacatactg gtggggaaca tgaccgtgac cgtactcgtg
     9001 gtggccagca cactactatg agcgagctga ttaaggagaa catgcacatg aagctgtaca
     9061 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     9121 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     9181 tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg
     9241 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     9301 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     9361 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     9421 agaaaacact cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg
     9481 aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca
     9541 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     9601 tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     9661 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaag tgaatcgttc
     9721 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     9781 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     9841 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     9901 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     9961 agatcactag tgcggccgcc tgcaggtcga ccatatggga gagctcaagc ttagcttgag
    10021 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
    10081 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
    10141 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
    10201 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
    10261 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
    10321 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
    10381 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
    10441 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
    10501 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
    10561 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
    10621 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
    10681 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
    10741 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
    10801 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
    10861 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
    10921 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
    10981 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
    11041 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
    11101 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
    11161 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
    11221 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
    11281 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
    11341 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
    11401 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
    11461 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
    11521 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
    11581 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
    11641 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
    11701 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
    11761 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
    11821 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
    11881 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
    11941 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
    12001 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
    12061 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
    12121 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
    12181 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
    12241 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
    12301 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
    12361 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
    12421 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
    12481 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
    12541 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
    12601 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
    12661 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
    12721 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
    12781 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
    12841 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
    12901 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
    12961 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
    13021 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
    13081 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
    13141 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
    13201 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
    13261 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
    13321 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
    13381 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
    13441 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
    13501 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
    13561 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
    13621 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
    13681 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
    13741 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
    13801 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
    13861 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
    13921 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
    13981 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    14041 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    14101 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
    14161 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    14221 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
    14281 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    14341 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    14401 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    14461 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    14521 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    14581 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    14641 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    14701 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    14761 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    14821 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    14881 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    14941 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    15001 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    15061 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg
    15121 atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact
    15181 tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaatcg cttgagttaa
    15241 cgccggcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt gccgactacc
    15301 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
    15361 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
    15421 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
    15481 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
    15541 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
    15601 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
    15661 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
    15721 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
    15781 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
    15841 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
    15901 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
    15961 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
    16021 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc ccccatgatg
    16081 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
    16141 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
    16201 ccaaaaaaac atgtcataac aagaagccat gaaaaccgcc actgcgccgt taccaccgct
    16261 gcgttcggtc aaggttctgg accagttgcg tgacggcagt tacgctactt gcattacagc
    16321 ttacgaaccg aacgaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca
    16381 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
    16441 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
    16501 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    16561 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    16621 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
    16681 gaattaacgc cgaattgaat tatcagcttg catgccggtc gatctagtaa catagatgac
    16741 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
    16801 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
    16861 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
    16921 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttga
    16981 ctctagggaa ttaattcctg aatcactgcg accggccctc ccgcgaccca gccgagcgag
    17041 cttagcgaac tgtggacgag aactgtgcca ccaagcgtaa ggccgttctc tcgcatttgc
    17101 cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg aaatcagctc ttgttcggtc
    17161 ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
    17221 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
    17281 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
    17341 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
    17401 tatacgcccg gagccttggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
    17461 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
    17521 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
    17581 tcaggacatt gttggagccg aatccgcgtg cacgagtgcc ggacttcggg gcagtcctcg
    17641 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
    17701 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
    17761 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
    17821 ccgcagcgat agcatccata gcctccgcga ccggctgaag aacagcgggc agttcggttt
    17881 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
    17941 cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
    18001 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
    18061 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
    18121 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
    18181 caggcttttt catagggggg attcgagttg agagtgaata tgagactcta attggatacc
    18241 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
    18301 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
    18361 taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa
    18421 acgtaaaacg gcttgtcccg cgtcatcggc ggggtcataa cgtgactccc ttaattctca
    18481 tgtatgataa ttcgagggta cccggggatc ctctagaggg cc 
//
