LOCUS       pCS2+cMyc_Dam_f_        5782 bp    DNA     circular     24-NOV-2016
DEFINITION  .
ACCESSION   urn.local...3f-5clzxyp
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/
            ORIGDB|GenBank VNTDATE|476633773| VNTDBDATE|476633773| LSOWNER|
            VNTNAME|pCS2plus| VNTAUTHORNAME|Arturo Gutierrez|
            VNTREPLTYPE|Phagemid VNTEXTCHREPL|Bacteria
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            #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 95 0 0 "")
            #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 29 0 0 "")
            #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") #7=(CRSite
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            #16=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 378 0 0 ""))
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FEATURES             Location/Qualifiers
     promoter        35..51
     CDS             95..1801
     CDS             95..127
                     /gene="Myc (3087-3116)"
                     /note="[pGEM-HeReg-PACa_cMyc]"
                     /codon_start=0
     misc_feature    <140..970
                     /note="DNA adenine methylase"
                     /note="Geneious type: CDS"
     misc_feature    500..502
                     /note="L122A"
     misc_feature    977..1051
                     /note="Flexible linker"
                     /note="Geneious type: CDS"
     CDS             <1058..>1801
     misc_feature    1771^1772
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1772..1798
                     /note="Geneious type: NLS"
     polyA_site      1832..2028
     misc_feature    2032..2061
     primer_bind     2077..2096
     misc_feature    complement(2130..2153)
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      2463..3129
     CDS             complement(3278..4138)
     promoter        4798..5782
ORIGIN      
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccaccggt caacatggag caaaagctga tctcggagga
      121 ggacctgggc ggctcaggca agaaaaatcg cgcttttttg aagtgggcag ggggcaagta
      181 tcccctgctt gatgatatta aacggcattt gcccaagggc gaatgtctgg ttgagccttt
      241 tgtaggtgcc gggtcggtgt ttctcaacac cgacttttct cgttatatcc ttgccgatat
      301 caatagcgac ctgatcagtc tctataacat tgtgaagatg cgtactgatg agtacgtaca
      361 ggccgcacgc gagctgtttg ttcccgaaac aaattgcgcc gaggtttact atcagttccg
      421 cgaagagttc aacaaaagcc aggatccgtt ccgtcgggcg gtactgtttt tatatttgaa
      481 ccgctacggt tacaacggcg cttgtcgtta caatctgcgc ggtgagttta acgtgccgtt
      541 cggccgctac aaaaaaccct atttcccgga agcagagttg tatcacttcg ctgaaaaagc
      601 gcagaatgcc tttttctatt gtgagtctta cgccgatagc atggcgcgcg cagatgatgc
      661 atccgtcgtc tattgcgatc cgccttatgc accgctgtct gcgaccgcca actttacggc
      721 gtatcacaca aacagtttta cgcttgaaca acaagcgcat ctggcggaga tcgccgaagg
      781 tctggttgag cgccatattc cagtgctgat ctccaatcac gatacgatgt taacgcgtga
      841 gtggtatcag cgcgcaaaat tgcatgtcgt caaagttcga cgcagtataa gcagcaacgg
      901 cggcacacgt aaaaaggtgg acgaactgct ggctttgtac aaaccaggag tcgtttcacc
      961 cgcgaaaaaa gctagcctga gcggtggagg cggttcaggc ggaggtggct ctggcggtgg
     1021 cggatcggga ggcggtggaa gtgcagccgc gactagtatg agtaaaggag aagaactttt
     1081 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggt acaaattttc
     1141 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     1201 cactactgga aaactacctg ttccatggcc aacacttgtc actactctca cttatggtgt
     1261 tcaatgcttt tcaagatatc cagatcatat gaagcggcac gacttcttca agagcgccat
     1321 gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac
     1381 acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat
     1441 cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca
     1501 caacgtatac atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg
     1561 ccacaacatc gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat
     1621 tggcgatgac cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1681 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cggctgctgg
     1741 gattacacat ggcatggatg aactatacaa accacctccc aagaggccca ggctggacta
     1801 atctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     1861 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1921 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1981 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     2041 gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     2101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2941 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4261 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4321 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4381 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4441 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4501 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4561 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4621 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4681 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     4741 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagtcgac
     4801 catagccaat tcaatatggc gtatatggac tcatgccaat tcaatatggt ggatctggac
     4861 ctgtgccaat tcaatatggc gtatatggac tcgtgccaat tcaatatggt ggatctggac
     4921 cccagccaat tcaatatggc ggacttggca ccatgccaat tcaatatggc ggacttggca
     4981 ctgtgccaac tggggagggg tctacttggc acggtgccaa gtttgaggag gggtcttggc
     5041 cctgtgccaa gtccgccata ttgaattggc atggtgccaa taatggcggc catattggct
     5101 atatgccagg atcaatatat aggcaatatc caatatggcc ctatgccaat atggctattg
     5161 gccaggttca atactatgta ttggccctat gccatatagt attccatata tgggttttcc
     5221 tattgacgta gatagcccct cccaatgggc ggtcccatat accatatatg gggcttccta
     5281 ataccgccca tagccactcc cccattgacg tcaatggtct ctatatatgg tctttcctat
     5341 tgacgtcata tgggcggtcc tattgacgta tatggcgcct cccccattga cgtcaattac
     5401 ggtaaatggc ccgcctggct caatgcccat tgacgtcaat aggaccaccc accattgacg
     5461 tcaatgggat ggctcattgc ccattcatat ccgttctcac gccccctatt gacgtcaatg
     5521 acggtaaatg gcccacttgg cagtacatca atatctatta atagtaactt ggcaagtaca
     5581 ttactattgg aaggacgcca gggtacattg gcagtactcc cattgacgtc aatggcggta
     5641 aatggcccgc gatggctgcc aagtacatcc ccattgacgt caatggggag gggcaatgac
     5701 gcaaatgggc gttccattga cgtaaatggg cggtaggcgt gcctaatggg aggtctatat
     5761 aagcaatgct cgtttaggga ac
//
