LOCUS       615-(pDestTol_Ub       11582 bp    DNA     circular     07-MAR-2011
DEFINITION  attB1-attB2-attB3-attB4.
ACCESSION   urn.local...1q-63rh3af
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/
            VNTDATE|517591782| VNTDBDATE|584009478| LSOWNER| VNTNAME|pCM283
            pDestTol2CG2_ubi-creERT2| VNTAUTHORNAME|Christian Mosimann|.
FEATURES             Location/Qualifiers
     misc_feature    1..1976
                     /note="Geneious type: Editing History Replacement"
     CDS             10..1719
     CDS             10..42
                     /gene="Myc (3087-3116)"
                     /note="[pGEM-HeReg-PACa_cMyc]"
                     /codon_start=0
     CDS             <55..>886
     primer_bind     795..814
     misc_feature    892..966
                     /note="Flexible linker"
                     /note="Geneious type: CDS"
     misc_feature    976..>1689
                     /note="mmGFP5"
                     /note="Geneious type: CDS"
     misc_feature    complement(1004..1025)
                     /note="Internal mmGFP 5' reverse primer"
     misc_feature    complement(1072..1092)
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1326..1345
     primer_bind     1499..1516
     misc_feature    complement(1649..1668)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1690..1716
                     /note="Geneious type: NLS"
     polyA_site      1750..1946
     misc_feature    1950..1979
     polyA_signal    1993..2190
     polyA_signal    complement(2249..2381)
     CDS             complement(2432..3148)
     misc_structure  complement(2944..2957)
     promoter        complement(3149..4047)
     misc_structure  complement(4054..4388)
     LTR             complement(4552..4570)
     misc_structure  complement(4571..4607)
     promoter        complement(4656..4675)
     primer_bind     complement(4691..4712)
     rep_origin      complement(5039..5712)
     CDS             complement(5860..6717)
     gene            7178..7463
     LTR             complement(7463..7479)
     misc_structure  complement(7652..7998)
     primer_bind     8010..8031
     misc_feature    8046..8066
     promoter        8088..11575
     exon            9479..9549
     primer_bind     9514..9536
     misc_feature    11450..11471
                     /note="AG377"
                     /note="Geneious type: primer_bind"
     misc_feature    11582^11583
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 ccggtcaaca tggagcaaaa gctgatctcg gaggaggacc tgggcggctc aggcaagaag
       61 aacagggcct tcctgaagtg ggctggcgga aagtacccac tgctggacga catcaagagg
      121 cacctgccca agggcgagtg cctggtcgag cctttcgtgg gagccggaag cgtgttcctg
      181 aacaccgact tcagccgcta catcctggcc gacatcaaca gcgacctgat cagcctgtac
      241 aacatcgtga agatgaggac cgacgagtac gtgcaggccg ctcgcgagct gttcgtgccc
      301 gagacaaact gcgccgaggt gtactaccag ttcagagaag agttcaacaa gagccaggac
      361 cccttccgca gggccgtgct gttcctgtac ctgaacagat acggatacaa cggcgcctgc
      421 agatacaacc tgaggggcga gttcaacgtg cccttcggaa gatacaagaa gccctacttc
      481 cccgaggccg agctgtacca cttcgccgag aaggcccaga acgcattctt ctactgcgag
      541 agctacgccg acagcatggc cagggccgac gacgcctctg tggtgtactg cgaccctccc
      601 tacgcccctc tgagcgccac cgccaacttc accgcctacc acaccaacag cttcaccctg
      661 gagcagcagg cccacctggc cgagatcgct gagggactgg tcgagaggca catccccgtg
      721 ctgatctcca accacgacac catgctgacc agagagtggt atcagagggc caagctgcac
      781 gtggtcaaag tgcgcagatc catcagcagc aacggcggaa ccagaaagaa ggtggacgag
      841 ctgctggccc tgtacaagcc cggcgtggtg tcccctgcca agaaggctag cctgagcggt
      901 ggaggcggtt caggcggagg tggctctggc ggtggcggat cgggaggcgg tggaagtgca
      961 gccgcgacta gtaccatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     1021 gttgaattag atggtgatgt taatgggtac aaattttctg tcagtggaga gggtgaaggt
     1081 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1141 ccatggccaa cacttgtcac tactctcact tatggtgttc aatgcttttc aagatatcca
     1201 gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata cgtgcaggag
     1261 aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt caagtttgag
     1321 ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga ggacggaaac
     1381 atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat catggccgac
     1441 aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga agacggcggc
     1501 gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatgaccc tgtcctttta
     1561 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1621 gaccacatgg tccttcttga gtttgtaacg gctgctggga ttacacatgg catggatgaa
     1681 ctatacaaac cacctcccaa gaggcccagg ctggactaat ctagaactat agtgagtcgt
     1741 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1801 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1861 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1921 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
     1981 accatcgatg atgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     2041 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2101 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2161 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     2221 gctgattatg atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat
     2281 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2341 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta
     2401 tagttctaga ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga
     2461 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     2521 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     2581 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     2641 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     2701 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     2761 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     2821 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     2881 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     2941 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     3001 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     3061 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     3121 tgaacagctc ctcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct
     3181 cctgggtcac tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt
     3241 ctataaaggt ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat
     3301 acaggagccc tgataactgc acaagtgctc agattccagc agggtggaaa atgagataaa
     3361 gtgtgcagat ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa
     3421 cgcattgaaa atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac
     3481 cagaccttta cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag
     3541 atgattttac aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt
     3601 caaagcaatt gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa
     3661 tttaatgtga catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc
     3721 acatgtttaa aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag
     3781 gcaaagctct tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg
     3841 ttaaatgtgt gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct
     3901 gtaatttatc taaagttaca actggctatg cctgattttt atcactattg aatcaatcat
     3961 tttaaagaat gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc
     4021 ttatttaaca caactgattt aagctttaga tctgcgaaga tacggccacg ggtgctcttg
     4081 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc
     4141 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc
     4201 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca
     4261 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg
     4321 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc
     4381 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
     4441 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
     4501 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
     4561 tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga
     4621 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     4681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5521 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6841 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6901 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6961 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     7021 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7081 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7141 gggagccccc gatttagagc ttgacgggga aagccgggct ggctaagaac tcatcagcct
     7201 ccccggtcca tctacccacg taccaatgca ccaattggcc acaatgacgg ctactacatg
     7261 gtgccattcc ttcctcttta taggaatgga gactacctcc tgtccaacaa ggctcttgga
     7321 tacgagtacg cctacctgtt ggacccaggt cattgcacaa caccagaaat gccctctgat
     7381 ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca ctctgttcca cacaggtcag
     7441 aggtttgtcc aggagttctt gacagaggtg taaaaagtac tcaaaaattt tactcaagtg
     7501 aaagtacaag tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat
     7561 cttacttgag taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt
     7621 aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac
     7681 attaaatgaa atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca
     7741 aactataaga cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat
     7801 ttaacttcag tagaagctga ttctcaaaat tgttagtgtc aagcctagct cttttggggc
     7861 tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg
     7921 tattagtctt gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt
     7981 ctgaaacaca ggccagatgg gccctcgagc aggaaacagc tatgaccatg attacgccaa
     8041 gctatcaact ttgtatagaa aagttggctc cgaattcgcc cttaaactcg agaccagcaa
     8101 agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat tctgagtaag
     8161 ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata aatgataaca
     8221 aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc ctcaaacagt
     8281 gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa ttggttcaga
     8341 agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc tgattccaca
     8401 atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac agcactcact
     8461 agctgcccta tatattttat tatattttac atatattatt ttatttattt agctctgagt
     8521 gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta cctcaggctc
     8581 agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct tgttttcaac
     8641 tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca tgcgactttt
     8701 ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat ataattatat
     8761 tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag atagtcagta
     8821 taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc tatatataag
     8881 tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa tgtagaatga
     8941 cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt gaaaacatcc
     9001 tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa catatacaaa
     9061 aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct ggcagttctg
     9121 tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca gattccacaa
     9181 gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac tgcttgtaga
     9241 ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat atgataatta
     9301 taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac catagcaatc
     9361 attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat ctggtcatgt
     9421 gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact ctcgaccatc
     9481 ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt ttcaacgcaa
     9541 atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta aaaccatagc
     9601 gtattacttt aattatagta aactttcgct ttctttatta caagagacgt tttgtgttga
     9661 ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac cagtaacttg
     9721 cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac gtcaaatagg
     9781 ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta tttttaattt
     9841 atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa agaacccgga
     9901 tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat tttaaaaata
     9961 attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa atgaggaaat
    10021 atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa ctggagaaaa
    10081 ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta gatgttttat
    10141 gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat aacagtaacg
    10201 ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg taagctacga
    10261 gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt aaataaaggc
    10321 tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag aaaaaatata
    10381 aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc tttttgttcc
    10441 tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc caaatgtttc
    10501 cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat aataattttg
    10561 gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca tgcttcagga
    10621 agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca ttacaaacta
    10681 ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc aaatcaaaat
    10741 ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag aaattgtttt
    10801 tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta aaattccaaa
    10861 atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa cagtcttatc
    10921 taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt ttaaatgact
    10981 gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc ttactggccc
    11041 caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca ttattattct
    11101 ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag atgtgcagtt
    11161 tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat gatgcacaaa
    11221 gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga ccctaaattt
    11281 tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata gtccactgtc
    11341 agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag ttttctatta
    11401 ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc attttcgggt
    11461 gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta gaacattgta
    11521 gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt tacagggatc
    11581 ca
//
