LOCUS       Exported                9066 bp ds-DNA     circular SYN 28-NOV-2016
DEFINITION  Ligation of NHEL.Puro-T2A.GFP into pB-CMV-DsRed-CAG-GFP.BS.with 
            repeats cut XhoI and  EcoRI.
ACCESSION   .
VERSION     .
KEYWORDS    pNHEJ-RPG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    330..989
                     /note="AMP"
     misc_feature    1087..1769
                     /note="ColE1 ori"
     misc_feature    2338..2650
                     /note="PB5'LTR"
     misc_feature    2338..2650
                     /note="PB 5'LTR"
     misc_feature    2753..2769
                     /note="pCAG pro '"
     misc_feature    2779..3367
                     /note="CMV IE Promoter"
     misc_feature    2837..3243
                     /note="Enhancer"
     misc_feature    3298..3317
                     /note="CMV-Foward"
     misc_feature    3361
                     /note="Transcription start point"
     misc_feature    3386..4066
                     /note="DsRed1"
     misc_feature    4071..4531
                     /note="Rabbit b-globin pA"
     misc_feature    4559..6277
                     /note="pCAG pro"
     misc_feature    6047..6284
                     /note="ORF-1''"
     misc_feature    6243..6262
                     /note="pCAG-F"
     misc_feature    6290..6315
                     /note="MCS"
     misc_feature    6290..6295
                     /note="Kozak"
     misc_feature    6293..7705
                     /note="ORF-1"
     misc_feature    6293..6295
                     /note="start codon"
     misc_feature    6317..6910
                     /note="Puro"
     misc_feature    6926..6988
                     /note="T2A"
     misc_feature    6926..6988
                     /note="T2A"
     misc_feature    6989..7705
                     /note="GFP"
     misc_feature    7732..7944
                     /note="PolyA"
     misc_feature    8126..8360
                     /note="PB3'LTR"
     misc_feature    8126..8360
                     /note="PB 3'LTR"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctgggtacc tcgcgcgact
     2221 tggtttgcca ttctttagcg cgcgtcgcgt cacacagctt ggccacaatg tggtttttgt
     2281 caaacgaaga ttctatgacg tgtttaaagt ttaggtcgag taaagcgcaa atctttttta
     2341 accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct
     2401 ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct
     2461 cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg
     2521 tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata
     2581 attatattgt tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt
     2641 tatagatatc gtgactaata tataataaaa tgggtagttc tttagacgat gagcatatcc
     2701 tctctgctct tctgcaaagc gatgacgagc ttgttggcta gcggtggcgg cctcgacatt
     2761 gattattgac tagagcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     2821 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2881 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2941 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3001 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3061 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3121 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3181 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3241 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3301 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     3361 tcagatccgc tagccggtcg ccaccatggt gcgctcctcc aagaacgtca tcaaggagtt
     3421 catgcgcttc aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg
     3481 cgagggcgag ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg
     3541 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
     3601 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
     3661 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3721 ctccctgcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
     3781 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     3841 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg
     3901 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
     3961 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt
     4021 ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgtaaggat ctttttccct
     4081 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     4141 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     4201 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     4261 catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg
     4321 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     4381 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     4441 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     4501 ctcttatgaa gatccctcga cctgcagccc aagcttatcg atactagcgg tggcggcctc
     4561 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4621 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4681 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4741 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4801 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4861 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4921 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     4981 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     5041 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     5101 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     5161 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     5221 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
     5281 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     5341 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     5401 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     5461 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     5521 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     5581 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     5641 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     5701 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     5761 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     5821 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     5881 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     5941 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     6001 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     6061 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     6121 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     6181 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     6241 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattca ccatggcggc
     6301 cgcgatatcg gatcccaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6361 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6421 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6481 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6541 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6601 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6661 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6721 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6781 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6841 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6901 gcccggtgcc gctcgagcta ccggtggcag tggagagggc agaggaagtc tgctaacatg
     6961 cggtgacgtc gaggagaatc ctggcccagt gagcaagggc gaggagctgt tcaccggggt
     7021 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     7141 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     7201 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7261 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     7441 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     7501 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7561 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     7621 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     7681 cggcatggac gagctgtaca agtaatctag ataactgatc ataatcagcc ataccacatt
     7741 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     7801 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     7861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7921 gtccaaactc atcaatgtat cttagtcgag ttaattaacg agagcataat attgatatgt
     7981 gccaaagttg tttctgactg actaataagt ataatttgtt tctattatgt ataggttaag
     8041 ctaattactt attttataat acaacatgac tgtttttaaa gtacaaaata agtttatttt
     8101 tgtaaaagag agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt
     8161 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     8221 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     8281 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     8341 gattatcttt ctagggttaa ataatagttt ctaatttttt tattattcag cctgctgtcg
     8401 tgaataccga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     8461 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8521 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8581 ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg
     8641 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8701 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8761 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8821 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8881 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8941 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9001 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     9061 atttcc
//
