LOCUS       Exported                5912 bp ds-DNA     circular SYN 28-NOV-2016
DEFINITION  pOP455 with N-terminal amino acids of HC modified. FINAL 2G4 
            CONSTRUCT.
ACCESSION   urn.local.MacBookAir.1412266516035.40
VERSION     .
KEYWORDS    pOP459
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     Serial Cloner Genbank Format SerialCloner_Type=DNA 
            SerialCloner_Comments= SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     412..442
                     /label=OP47_PCR
                     /note="OP47_PCR"
     terminator      419..658
                     /label=CYC tt
                     /note="CYC tt terminator"
     primer_bind     482..508
                     /label=JP121
                     /note="JP121"
     misc_feature    complement(488..509)
                     /label=spR002
                     /note="spR002"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(581..602)
                     /label=OP258
                     /note="OP258"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(716..1344)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     primer_bind     717..736
                     /label=JP109
                     /note="JP109"
     misc_feature    complement(786..805)
                     /label=mylP133
                     /note="mylP133"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     831..850
                     /label=pL131-f09
                     /note="pL131-f09"
     primer_bind     1230..1255
                     /label=OP362
                     /note="OP362"
     primer_bind     1234..1258
                     /label=OP384
                     /note="OP384"
     primer_bind     1234..1257
                     /label=JP110
                     /note="JP110"
     misc_feature    complement(1315..1346)
                     /label=OP378
                     /note="OP378"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1348..1368
                     /label=OP363
                     /note="OP363"
     primer_bind     1354..1378
                     /label=pL131-f10
                     /note="pL131-f10"
     misc_feature    1418..1494
                     /label=BxbI attB
                     /note="BxbI attB"
     misc_feature    complement(1472..1491)
                     /label=NR12
                     /note="NR12"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1472..1491)
                     /label=NR39
                     /note="NR39"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1500..1604
                     /label=UASrpg
                     /note="UASrpg"
     misc_feature    1610..2548
                     /label=pAOX1
                     /note="pAOX1"
     primer_bind     2461..2483
                     /label=OP752
                     /note="OP752"
     primer_bind     2465..2497
                     /label=OP480
                     /note="OP480"
     primer_bind     2522..2579
                     /label=OP730
                     /note="OP730"
     CDS             2549..2815
                     /label=alpha factor
                     /note="alpha factor"
     CDS             2816..4162
                     /label=2G4 Heavy Chain
                     /note="2G4 Heavy Chain"
     CDS             2816..3193
                     /label=Variable region
                     /note="Variable region"
     CDS             3194..4162
                     /label=Constant region IgG-1. Uniprot P01857, less first 
                     7aa which were already in the variable region
                     /note="Constant region IgG-1. Uniprot P01857, less fir"
     primer_bind     3296..3319
                     /label=OP852
                     /note="OP852"
     misc_feature    4163..4225
                     /label=GSG-T2A
                     /note="GSG-T2A"
                     /note="Geneious type: motif"
     CDS             4226..4492
                     /label=alpha factor
                     /note="alpha factor"
     CDS             4493..5137
                     /label=2G4 Light Chain
                     /note="2G4 Light Chain"
     CDS             4493..4816
                     /label=Variable region
                     /note="Variable region"
     CDS             4817..5137
                     /label=Constant region (Kappa)
                     /note="Constant region (Kappa)"
     misc_feature    5138..5384
                     /label=AOX1 terminator
                     /note="AOX1 terminator"
     misc_feature    complement(5144..5164)
                     /label=OP329
                     /note="OP329"
                     /note="Geneious type: primer_bind_reverse"
     promoter        5405..5811
                     /label=TEF1 promoter
                     /note="TEF1 promoter"
     primer_bind     5414..5433
                     /label=pTEF1-f
                     /note="pTEF1-f"
     promoter        5817..5883
                     /label=EM7 promoter
                     /note="EM7 promoter"
     promoter        5817..5883
                     /label=mPGK Prom
                     /note="mPGK Prom promoter"
     CDS             5884..346
                     /label=ZeoR
                     /note="ZeoR"
ORIGIN
        1 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
       61 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
      121 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
      181 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
      241 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
      301 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tccgacggcg
      361 gcccacgggt cccaggcctc ggagatccgt cccccttttc ctttgtcgat atcatgtaat
      421 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
      481 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
      541 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
      601 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgca
      661 agctggagac caacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      901 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
      961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     1201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1381 aagggatttt ggtcatgaga tcagatctga gctcgtttgg ccgtggccgt gctcgtcctc
     1441 gtcggccggc ttgtcgacga cggcggtctc cgtcgtcagg atcatccggg ccacaagctt
     1501 gctgacagaa gcctcaagaa aaaaaaaatt cttcttcgac tatgctggag gcagagatga
     1561 tcgagccggt agttaactat atatagctaa attggttcca tcacctcgag atctaacatc
     1621 caaagacgaa aggttgaatg aaaccttttt gccatccgac atccacaggt ccattctcac
     1681 acataagtgc caaacgcaac aggaggggat acactagcag cagaccgttg caaacgcagg
     1741 acctccactc ctcttctcct caacacccac ttttgccatc gaaaaaccag cccagttatt
     1801 gggcttgatt ggagctcgct cattccaatt ccttctatta ggctactaac accatgactt
     1861 tattagcctg tctatcctgg cccccctggc gaggttcatg tttgtttatt tccgaatgca
     1921 acaagctccg cattacaccc gaacatcact ccagatgagg gctttctgag tgtggggtca
     1981 aatagtttca tgttccccaa atggcccaaa actgacagtt taaacgctgt cttggaacct
     2041 aatatgacaa aagcgtgatc tcatccaaga tgaactaagt ttggttcgtt gaaatgctaa
     2101 cggccagttg gtcaaaaaga aacttccaaa agtcggcata ccgtttgtct tgtttggtat
     2161 tgattgacga atgctcaaaa ataatctcat taatgcttag cgcagtctct ctatcgcttc
     2221 tgaaccccgg tgcacctgtg ccgaaacgca aatggggaaa cacccgcttt ttggatgatt
     2281 atgcattgtc tccacattgt atgcttccaa gattctggtg ggaatactgc tgatagccta
     2341 acgttcatga tcaaaattta actgttctaa cccctacttg acagcaatat ataaacagaa
     2401 ggaagctgcc ctgtcttaaa cctttttttt tatcatcatt attagcttac tttcataatt
     2461 gcgactggtt ccaattgaca agcttttgat tttaacgact tttaacgaca acttgagaag
     2521 atcaaaaaac aactaattat tcgaaacgat gagattccct tcaattttta cagccgttct
     2581 gttcgctgct tcttccgctc tagccgcacc cgtgaatact acaactgagg atgagactgc
     2641 ccagattcct gcagaggccg tcatcggcta ctccgattta gaaggtgatt ttgatgttgc
     2701 tgtgctgcca ttttctaact ctaccaacaa cggactgctt tttattaata caacaattgc
     2761 atccatagca gccaaggagg agggtgtatc ccttgaaaaa agagaagctg aagccgccct
     2821 tgaggagtct ggtggtggtc tgatgcagcc agggggtagt atgaagttat cttgtgtggc
     2881 atccggcttt actttcagta attactggat gaactgggtt cgacaaagtc cagagaaagg
     2941 cctggagtgg gttgccgaaa ttcgtttgaa atctaataac tacgcaacac attacgcaga
     3001 gagtgttaaa ggccgattca ctattagtag agatgattct aagcgttcag tctacttgca
     3061 aatgaatacg ttacgtgctg aagacacagg aatttactac tgtacaagag gtaatggaaa
     3121 ctacagagcc atggattact ggggtcaagg tacttcagtc actgttagtt cagccaaaac
     3181 tacacctcct tctgtattcc ccttggctcc atcctccaaa tccacttccg gcggtacagc
     3241 agctttgggc tgtcttgtga aggattactt tccagagcca gttacagtct cttggaattc
     3301 tggagcactg accagtggcg tccatacgtt tcctgcagtc ttgcagtcca gtgggcttta
     3361 tagtctatca tctgtcgtta ctgttccctc aagttctctg gggacccaaa cttacatttg
     3421 taatgtgaac cacaaacctt ctaacaccaa ggtggacaag aaggttgaac caaaatcttg
     3481 cgacaaaaca cacacttgtc ctccatgtcc tgccccagaa ttgcttggtg gtccctccgt
     3541 ctttttgttt cccccaaaac ccaaggacac tcttatgatt tccagaacac ctgaggttac
     3601 ctgtgttgtt gtggatgtta gtcacgagga ccctgaggtt aagttcaatt ggtacgtgga
     3661 tggtgttgaa gtgcacaatg ctaaaacaaa gccaagggaa gagcaataca attctaccta
     3721 tcgagtggtt tctgtattaa ctgtgttaca tcaagactgg cttaacggaa aagaatataa
     3781 gtgcaaagtt tcaaacaagg cattaccagc tccaattgag aaaacaatta gtaaagccaa
     3841 gggtcagcct agagagccac aggtttacac tttgccacct tcacgtgatg aactaacaaa
     3901 gaatcaagtt agtctgacct gtttagttaa gggattttac ccttccgata ttgccgttga
     3961 atgggaatct aatggtcagc ctgaaaacaa ctataagaca actcctccag tccttgattc
     4021 agacggttca tttttcttgt actctaaatt gactgttgac aaatctagat ggcagcaagg
     4081 taacgttttc tcttgctctg ttatgcacga ggctcttcac aatcactaca cgcagaaaag
     4141 tttgtccctg tcacctggca agggtagtgg tgaaggtaga ggaagtcttc ttacatgtgg
     4201 tgatgtcgaa gaaaatccag gaccaatgcg ttttccatca atatttaccg ccgtattgtt
     4261 tgcagcttct tctgctttag ctgctccagt gaatacaacc acagaggacg agaccgcaca
     4321 gatccctgca gaggctgtca ttggttactc cgacttagag ggagactttg atgtggccgt
     4381 cttgcccttt tctaatagta ctaacaatgg attgctgttc attaatacta caatcgcaag
     4441 tattgctgct aaggaagaag gagtcagttt ggaaaaaaga gaagccgagg ctgacattca
     4501 gatgactcag tccccagctt cattatccgt ttctgttgga gaaaccgtat caattacttg
     4561 tcgtgccagt gaaaatatat atagttcact ggcttggtat caacaaaagc aaggaaaatc
     4621 accacaactg cttgtttatt cagctacgat cttggctgat ggagttccat cacgtttcag
     4681 tggttctgga agtgggacac agtactcctt aaaaatcaac tcattgcaat cagaggattt
     4741 cggaacctat tactgtcagc atttctgggg taccccatat acttttgggg gtggaaccaa
     4801 actggaaatt aagcgtactg tagccgcacc cagtgtcttc atttttcctc catctgacga
     4861 acaactgaaa tctggtaccg cttccgtcgt ctgtctgctt aacaactttt atcctagaga
     4921 ggccaaagtt caatggaagg ttgataatgc tttgcaatct ggcaactccc aagaatctgt
     4981 gacagagcag gattccaaag attccactta ctcattatct tctacactaa cattgagtaa
     5041 agccgattat gagaagcaca aagtgtacgc atgcgaggtg acacatcagg gtttgtcttc
     5101 tccagttact aaatctttca accgaggtga atgctagtca agaggatgtc agaatgccat
     5161 ttgcctgaga gatgcaggct tcatttttga tactttttta tttgtaacct atatagtata
     5221 ggattttttt tgtcattttg tttcttctcg tacgagcttg ctcctgatca gcctatctcg
     5281 cagctgatga atatcttgtg gtaggggttt gggaaaatca ttcgagtttg atgtttttct
     5341 tggtatttcc cactcctctt cagagtacag aagattaagt gagaccttcg tttgtgcgga
     5401 tcccccacac accatagctt caaaatgttt ctactccttt tttactcttc cagattttct
     5461 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaattttcc
     5521 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
     5581 agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
     5641 ttttcttgaa attttttttt ttagtttttt tctctttcag tgacctccat tgatatttaa
     5701 gttaataaac ggtcttcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac
     5761 tttttttact tcttgttcat tagaaagaaa gcatagcaat ctaatctaag gggcggtgtt
     5821 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     5881 accatggcca agttgaccag tgccgttccg gt
//
