LOCUS       Exported                5757 bp ds-DNA     circular SYN 21-NOV-2016
DEFINITION  pOP512 with modified gbOP58, generated by PCR of gbOP58 with 
            OP1058/OP1060, cloned in between BamHI/SalI.
ACCESSION   .
VERSION     .
KEYWORDS    pOP549
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /note="source synthetic DNA construct"
     misc_feature    complement(55..771)
                     /label=GFP
                     /note="GFP"
                     /note="GFPmut3b_RSR;color: #cc0000; direction: 
                     LEFT;Geneious type: CDS"
     misc_feature    complement(55..89)
                     /label=OP641
                     /note="OP641"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     55..88
                     /label=OP308
                     /note="OP308"
     primer_bind     55..80
                     /label=BamHI-gfp-R
                     /note="BamHI-gfp-R"
     misc_feature    complement(167..194)
                     /label=OP599
                     /note="OP599"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(232..255)
                     /label=OP530
                     /note="OP530"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(433..457)
                     /label=OP825
                     /note="OP825"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(440..463)
                     /label=NR18
                     /note="NR18"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     721..748
                     /label=OP601
                     /note="OP601"
     misc_feature    complement(744..768)
                     /label=KpnI-gfp-F
                     /note="KpnI-gfp-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(744..768)
                     /label=KpnI-gfp-F2
                     /note="KpnI-gfp-F2"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     768..791
                     /label=OP227
                     /note="OP227"
     misc_feature    complement(772..822)
                     /label=RBS
                     /note="RBS"
                     /note="rbsKDL025;color: #cc0000; direction: LEFT;Geneious 
                     type: misc_RNA"
     primer_bind     772..797
                     /label=OP290
                     /note="OP290"
     primer_bind     772..797
                     /label=OP640
                     /note="OP640"
     misc_feature    complement(772..797)
                     /label=OP305
                     /note="OP305"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(797..822)
                     /label=OP245
                     /note="OP245"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(798..822)
                     /label=OP283
                     /note="OP283"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(823..896)
                     /label=PLtet0
                     /note="PLtet0"
                     /note="PL(tetO);color: #cc0000; direction: LEFT;Geneious 
                     type: promoter"
     primer_bind     823..843
                     /label=mmD46
                     /note="mmD46"
     primer_bind     823..841
                     /label=NR2
                     /note="NR2"
     misc_feature    complement(830..851)
                     /label=NR14
                     /note="NR14"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(849..854)
                     /label=-10
                     /note="-10"
                     /note="-10 box;color: #cc0000; direction: LEFT"
     misc_feature    complement(853..871)
                     /label=Otet2
                     /note="Otet2"
                     /note="Otet2;color: #cc0000; direction: LEFT"
     misc_feature    complement(858..871)
                     /label=NheI-pLtet-F
                     /note="NheI-pLtet-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(872..877)
                     /label=-35
                     /note="-35"
                     /note="-35 Box;color: #cc0000; direction: LEFT"
     misc_feature    complement(878..896)
                     /label=Otet2
                     /note="Otet2"
                     /note="Otet2;color: #cc0000; direction: LEFT"
     misc_feature    complement(883..896)
                     /label=NheI-pLtet-F
                     /note="NheI-pLtet-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(904..947)
                     /label=OP224
                     /note="OP224"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     933..977
                     /label=OP223
                     /note="OP223"
     misc_feature    complement(939..2021)
                     /label=LacI
                     /note="LacI"
                     /note="lacI-RSR;color: #cc0000; direction: LEFT;Geneious 
                     type: CDS"
     modified_base   940
                     /label=changed to T
                     /note="changed to T"
     primer_bind     1148..1171
                     /label=OP156
                     /note="OP156"
     misc_feature    complement(1151..1170)
                     /label=lacZYA-KI-Ck-F
                     /note="lacZYA-KI-Ck-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1160..1179)
                     /label=lacZ-UpCk-F
                     /note="lacZ-UpCk-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1197..1216)
                     /label=lacZ-KO-Ck-F
                     /note="lacZ-KO-Ck-F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1210..1232
                     /label=OP449
                     /note="OP449"
     misc_feature    complement(1284..1303)
                     /label=lacZ-UpCk-New-F
                     /note="lacZ-UpCk-New-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1590..1610)
                     /label=OP822
                     /note="OP822"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1824..1848
                     /label=OP450
                     /note="OP450"
     primer_bind     1978..1998
                     /label=pL131-f04
                     /note="pL131-f04"
     misc_feature    complement(2022..2051)
                     /label=RBS
                     /note="RBS"
                     /note="rbsKDL026;color: #cc0000; direction: LEFT;Geneious 
                     type: misc_RNA"
     misc_feature    complement(2026..2051)
                     /label=OP310
                     /note="OP310"
                     /note="Geneious type: primer_bind_reverse"
     promoter        complement(2070..2143)
                     /label=pLtetO
                     /note="pLtetO promoter"
     promoter        2162..2236
                     /label=Ptrc-2
                     /note="Ptrc-2 promoter"
     misc_feature    2181..2197
                     /label=lambdaCI OR_1
                     /note="lambdaCI OR_1"
     misc_feature    2236..2237
                     /label=GTCGA
                     /note="GTCGA"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    2238..2269
                     /label=RBS
                     /note="RBS"
                     /note="rbsKDL028;color: #cc0000; direction: RIGHT;Geneious 
                     type: misc_RNA"
     misc_feature    2270..2893
                     /label=tetR
                     /note="tetR"
                     /note="tetR_RSR;color: #cc0000; direction: RIGHT;Geneious 
                     type: CDS"
     primer_bind     2304..2324
                     /label=BC_4
                     /note="BC_4"
     primer_bind     complement(2349..2370)
                     /label=JP119
                     /note="JP119"
     misc_feature    complement(2349..2370)
                     /label=JP119
                     /note="JP119"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2349..2370)
                     /label=OP239
                     /note="OP239"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2529..2555)
                     /label=OP824
                     /note="OP824"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2669..2691)
                     /label=OP154
                     /note="OP154"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2755..2783
                     /label=OP228
                     /note="OP228"
     primer_bind     complement(2802..2823)
                     /label=JP118
                     /note="JP118"
     misc_feature    complement(2802..2823)
                     /label=JP118
                     /note="JP118"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2845..2899)
                     /label=OP226
                     /note="OP226"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2885..2930
                     /label=OP225
                     /note="OP225"
     modified_base   2892
                     /label=changed to A
                     /note="changed to A"
     misc_feature    2936..3010
                     /label=Ptrc-2
                     /note="Ptrc-2"
                     /note="Ptrc-2;color: #cc0000; direction: RIGHT;Geneious 
                     type: promoter"
     modified_base   2937
                     /label=deleted
                     /note="deleted"
     misc_feature    2949..2954
                     /label=-35
                     /note="-35"
                     /note="-35 Box;color: #cc0000; direction: RIGHT"
     misc_feature    2972..2977
                     /label=-10
                     /note="-10"
                     /note="-10 box;color: #cc0000; direction: RIGHT"
     misc_feature    complement(2979..2998)
                     /label=OP269
                     /note="OP269"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2979..2998)
                     /label=lacI-Ck-R
                     /note="lacI-Ck-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2984..3004
                     /label=Olac
                     /note="Olac"
                     /note="Olac;color: #cc0000; direction: RIGHT"
     misc_feature    3017..3052
                     /label=rbs
                     /note="rbs"
                     /note="rbsKDL027;color: #cc0000; direction: RIGHT;Geneious 
                     type: misc_RNA"
     misc_feature    3053..3763
                     /label=mCherry
                     /note="mCherry"
                     /note="mCherry;color: #cc0000; direction: RIGHT;Geneious 
                     type: CDS"
     primer_bind     3053..3073
                     /label=OP299
                     /note="OP299"
     primer_bind     3053..3067
                     /label=rcD59
                     /note="rcD59"
     misc_feature    complement(3375..3394)
                     /label=mCherry_reverse_complementary_F
                     /note="mCherry_reverse_complementary_F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3657..3680)
                     /label=pL037-bp392-bp415-f (D1162)
                     /note="pL037-bp392-bp415-f (D1162)"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3818..3922
                     /label=T1
                     /note="T1"
                     /note="T1;transcriptional terminator from rrnB 
                     operon;color: #cc0000; direction: RIGHT;Geneious type: 
                     terminator"
     primer_bind     3818..3842
                     /label=NR36
                     /note="NR36"
     primer_bind     3818..3838
                     /label=BamHI-TAA-ClaI-T1-F
                     /note="BamHI-TAA-ClaI-T1-F"
     primer_bind     3870..3892
                     /label=OP823
                     /note="OP823"
     misc_feature    complement(3902..3922)
                     /label=NR37
                     /note="NR37"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3903..3922)
                     /label=Pro-at-csgA-KI-R
                     /note="Pro-at-csgA-KI-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3903..3922)
                     /label=KIatPlacZYA-T1-R
                     /note="KIatPlacZYA-T1-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3903..3922)
                     /label=Pro-attB-KI-R
                     /note="Pro-attB-KI-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3903..3922)
                     /label=Pro-at-lacI-KI-R
                     /note="Pro-at-lacI-KI-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3905..3927)
                     /label=OP962
                     /note="OP962"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3910..3922)
                     /label=AvrII-T1-R
                     /note="AvrII-T1-R"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      3928..4685
                     /label=p15A
                     /note="p15A"
     primer_bind     4686..4709
                     /label=OP961
                     /note="OP961"
     misc_feature    4696..4801
                     /label=T0
                     /note="T0"
                     /note="T0;transcriptional terminator from phage 
                     lambda;color: #cc0000; direction: RIGHT;Geneious type: 
                     terminator"
     primer_bind     4696..4722
                     /label=Pro-at-lacI-KI-F
                     /note="Pro-at-lacI-KI-F"
     primer_bind     4696..4722
                     /label=Pro-attB-KI-F
                     /note="Pro-attB-KI-F"
     primer_bind     4696..4722
                     /label=Pro-at-csgA-KI-F
                     /note="Pro-at-csgA-KI-F"
     primer_bind     4696..4715
                     /label=gt0-Amp-F
                     /note="gt0-Amp-F"
     misc_feature    complement(4827..5621)
                     /label=KanR
                     /note="KanR"
                     /note="KanR;color: #cc0000; direction: LEFT;Geneious type: 
                     CDS"
     primer_bind     5687..5706
                     /label=kan-3-F
                     /note="kan-3-F"
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tcacttgaat tcgataccca gctgttattt
       61 gtatagttca tccatgccat gtgtaatccc agcagcggtt acaaactcaa gaaggaccat
      121 gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt
      181 gtctggtaaa aggacagggc catcgccaat cggagtattt tgttgataat ggtctgctag
      241 ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt
      301 ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc
      361 aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt
      421 atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat
      481 agttctttcc tgtacgtaac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat
      541 gtgatctggg tatctcgcaa agcattgaac accataaccg aaagtagtga caagtgttgg
      601 ccacggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt
      661 tgcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa
      721 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttacgca tgtgcactac
      781 ctccttggtt gtctatgcta tgctgatcta caactggcat gcggtcagtg cgtcctgctg
      841 atgtgctcag tatctctatc actgataggg atgtcaatct ctatcactga tagggaaacg
      901 tttcgcagaa gcttccgcaa ggtaccactt tgccgcggtt actgcccgct ttccagtcgg
      961 gaaacctgtc gtgccagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1021 gtattgggag ccagggtggt ttttcttttc accagtgaga cgggcaacag ttgattgccc
     1081 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     1141 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     1201 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
     1261 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     1321 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     1381 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     1441 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     1501 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     1561 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     1621 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ctgcgcgaga
     1681 agattgtgaa ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     1741 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     1801 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     1861 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     1921 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     1981 ccggcatact ctgcgacatc gtacagcgtt actggtttca ttgtacaccc tctccttata
     2041 ttgggaccta tggcactaac gttcgtaggg gtcagtgcgt cctgctgatg tgctcagtat
     2101 ctctatcact gatagggatg tcaatctcta tcactgatag ggacggccgc ccgttccatg
     2161 gctgaaatga gctgttgaca atggagaccg ccactattat aatgtgtgga attgtgagcg
     2221 gataacaatt tcacacccgc cttcggcgaa gctagggacg agagctagca tgtctcgttt
     2281 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
     2341 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
     2401 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
     2461 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
     2521 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
     2581 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
     2641 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
     2701 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
     2761 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
     2821 agccttctta ttcggccttg aattgatcat ctgcggatta gaaaaacaac ttaaatgtga
     2881 aagtgggtct taagagctcg gactgcttaa gtcgctccat atgctcgttc ccgggctgaa
     2941 atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg agcggataac
     3001 aatttcacac caattgagta ttaactatcg ttcaactgat agggaggcgc cgatggtgag
     3061 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggttcacat
     3121 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3181 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     3241 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3301 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3361 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcaag acggcgagtt
     3421 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3481 gaagactatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgcgctgaa
     3541 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     3601 gaccacctac aaggccaaga agcccgtgca actgcccggc gcgtacaacg tcaacatcaa
     3661 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     3721 gggccgccac tccaccggcg gcatggacga gctgtataag taagatatct atcgccctag
     3781 ggaccgtctc gagagaatca atattaatcc aacgcgtggc atcaaataaa acgaaaggct
     3841 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     3901 aggacaaatc cgccgcccta gacttagcgg tcgttcgact gcggcgagcg gaaatggctt
     3961 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     4021 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     4081 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4141 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     4201 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     4261 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     4321 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     4381 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     4441 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     4501 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     4561 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     4621 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     4681 ttgttcatgg ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     4741 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     4801 ccaattctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4861 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4921 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     4981 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     5041 ggcaagcagg catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat acgcgccttg
     5101 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     5161 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     5221 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     5281 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     5341 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagccgc gcaaggaacg
     5401 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     5461 gacaggtcgg tcttgacaaa aagaaccggg cgaccctgcg ctgacagccg gaacacggcg
     5521 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5581 gccgccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5641 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     5701 cagtttactt tgcagggctt cccaacctta ccagagggcg gcccaactgg caattcc
//
