LOCUS       Exported                7091 bp ds-DNA     circular SYN 21-NOV-2016
DEFINITION  pOP513 with pLac/ara-1 - lambdaCI cloned in.
ACCESSION   U02374
VERSION     .
KEYWORDS    pOP523
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Kovach ME, Phillips RW, Elzer PH, Roop RM, II, Peterson KM.
  TITLE     pBBR1MCS: a broad-host-range cloning vector
  JOURNAL   BioTechniques 16 (5), 800-802 (1994)
  PUBMED    8068328
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, II, 
            Peterson KM.
  TITLE     Four new derivatives of the broad-host-range cloning vector 
            pBBR1MCS, carrying different antibiotic-resistance cassettes
  JOURNAL   Gene 166 (1), 175-176 (1995)
  PUBMED    8529885
REFERENCE   3  (bases 1 to 7091)
  AUTHORS   Kovach ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-1993) Michael E. Kovach, Microbiology & 
            Immunology, Louisiana State University Medical Center-Shreveport, 
            1501 Kings Highway, Shreveport, LA 71130, USA
REFERENCE   4  (bases 1 to 7091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     On Dec 11, 1993 this sequence version replaced gi:407280.
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4080..4079
                     /organism="Cloning vector pBBR1MCS"
                     /plasmid="pBBR1MCS"
                     /lab_host="Bordetella bronchiseptica"
                     /mol_type="genomic DNA"
                     /label=Source Cloning vector pBBR1MCS
                     /note="Source Cloning vector pBBR1MCS"
                     /db_xref="taxon:31789"
     primer_bind     13..34
                     /label=OP713
                     /note="OP713"
     primer_bind     95..118
                     /label=JP80
                     /note="JP80"
     primer_bind     172..195
                     /label=D119
                     /note="D119"
     primer_bind     172..195
                     /label=pRS403-T7-seq-f (D1119)
                     /note="pRS403-T7-seq-f (D1119)"
     misc_feature    complement(172..195)
                     /label=OP23
                     /note="OP23"
                     /note="Geneious type: primer_bind_reverse"
     promoter        208..282
                     /label=ptrc-2
                     /note="ptrc-2 promoter"
     misc_feature    complement(251..270)
                     /label=OP269
                     /note="OP269"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(251..270)
                     /label=lacI-Ck-R
                     /note="lacI-Ck-R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     281..300
                     /label=OP315
                     /note="OP315"
     ncRNA           283..314
     CDS             315..1193
                     /label=araC
                     /note="araC"
     misc_feature    complement(426..449)
                     /label=OP820
                     /note="OP820"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     655..678
                     /label=pL037-bp1832-bp1855-f (D1164)
                     /note="pL037-bp1832-bp1855-f (D1164)"
     primer_bind     935..954
                     /label=OP1078
                     /note="OP1078"
     misc_feature    complement(1166..1193)
                     /label=OP819
                     /note="OP819"
                     /note="Geneious type: primer_bind_reverse"
     terminator      1194..1298
                     /label=T1 terminator
                     /note="T1 terminator"
     primer_bind     1194..1218
                     /label=NR36
                     /note="NR36"
     primer_bind     1194..1214
                     /label=BamHI-TAA-ClaI-T1-F
                     /note="BamHI-TAA-ClaI-T1-F"
     primer_bind     1246..1268
                     /label=OP823
                     /note="OP823"
     misc_feature    complement(1278..1298)
                     /label=NR37
                     /note="NR37"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1279..1298)
                     /label=Pro-at-csgA-KI-R
                     /note="Pro-at-csgA-KI-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1279..1298)
                     /label=KIatPlacZYA-T1-R
                     /note="KIatPlacZYA-T1-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1279..1298)
                     /label=Pro-attB-KI-R
                     /note="Pro-attB-KI-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1279..1298)
                     /label=Pro-at-lacI-KI-R
                     /note="Pro-at-lacI-KI-R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1286..1298)
                     /label=AvrII-T1-R
                     /note="AvrII-T1-R"
                     /note="Geneious type: primer_bind_reverse"
     promoter        1373..1858
                     /label=pLac/ara-1
                     /note="pLac/ara-1 promoter"
     misc_feature    1795..1800
                     /label=-35
                     /note="-35"
     misc_feature    1819..1824
                     /label=-10
                     /note="-10"
     misc_RNA        1884..1913
                     /label=RBS
                     /note="RBS"
     primer_bind     1884..1909
                     /label=OP310
                     /note="OP310"
     CDS             1914..2627
                     /label=lambdaCI
                     /note="lambdaCI"
     misc_feature    complement(2634..2653)
                     /label=KIatPlacZYA-Up-F
                     /note="KIatPlacZYA-Up-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2635..2658)
                     /label=gNBNLacZ-T1-F
                     /note="gNBNLacZ-T1-F"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2731..2753)
                     /label=OP16
                     /note="OP16"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2742..2767)
                     /label=OP288
                     /note="OP288"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2774..2793
                     /label=OP269
                     /note="OP269"
     primer_bind     2774..2793
                     /label=lacI-Ck-R
                     /note="lacI-Ck-R"
     misc_feature    complement(2776..2798)
                     /label=pL131-f07
                     /note="pL131-f07"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2789..2811
                     /label=OP600
                     /note="OP600"
     misc_feature    complement(2834..2864)
                     /label=OP448
                     /note="OP448"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2866..2887)
                     /label=OP1011
                     /note="OP1011"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2976..2985
                     /label=Misc. feature
                     /note="Misc. feature"
                     /note="Geneious type: Misc._feature;complement of 5' end of
                     PCR primer (5' TTTTATGCATgcgcccaatacgcaaa 3') used to 
                     amplify pBlusescript II KS- polylinker"
     misc_feature    complement(3009..3032)
                     /label=pBAC-step2-transf-bp9125-9148f (D1276)
                     /note="pBAC-step2-transf-bp9125-9148f (D1276)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3691..3712
                     /label=NR22
                     /note="NR22"
     primer_bind     3728..3748
                     /label=OP1010
                     /note="OP1010"
     misc_feature    complement(3819..3840)
                     /label=NR1
                     /note="NR1"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3821..3844)
                     /label=JP114
                     /note="JP114"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3860..3885)
                     /label=gAmp-proD-F
                     /note="gAmp-proD-F"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN
        1 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
       61 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      181 cgactcacta tagggcgaat tggagctctg aaatgagctg ttgacaatta atcatccggc
      241 tcgtataatg tgtggaattg tgagcggata acaatttcac accgccttcg gcgaagctag
      301 ggacgagagc tagcatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta
      361 acgcccatct ggtggcgggt ttaacgccga ttgaggccaa cggttatctc gattttttta
      421 tcgaccgacc gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcaggggg
      481 tggtgaaaaa tcagggacga gaatttgtct gccgaccggg tgatattttg ctgttcccgc
      541 caggagagat tcatcactac ggtcgtcatc cggaggctcg cgaatggtat caccagtggg
      601 tttactttcg tccgcgcgcc tactggcatg aatggcttaa ctggccgtca atatttgcca
      661 atacgggttt ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc
      721 aaatcattaa cgccgggcaa ggggaagggc gctattcgga gctgctggcg ataaatctgc
      781 ttgagcaatt gttactgcgg cgcatggaag cgattaacga gtcgctccat ccgccgatgg
      841 ataatcgggt acgcgaggct tgtcagtaca tcagcgatca cctggcagac agcaattttg
      901 atatcgccag cgtcgcacag catgtttgct tgtcgccgtc gcgtctgtca catcttttcc
      961 gccagcagtt agggattagc gtcttaagct ggcgcgagga ccaacgtatc agccaggcga
     1021 agctgctttt gagcactacc cggatgccta tcgccaccgt cggtcgcaat gttggttttg
     1081 acgatcaact ctatttctcg cgagtattta aaaaatgcac cggggccagc ccgagcgagt
     1141 ttcgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca taaggcatca
     1201 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1261 gaacgctctc ctgagtagga caaatccgcc gccctagagc ccggatgatc ctgacagctc
     1321 caccgcggtg gcggccgctc tagaactagt ggttgaccag acaaaccacg acaattgtga
     1381 gcggataaca atttcacaca gggccctcgg acaccgagga gaatgtcaag aggcgaacac
     1441 acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt gccctgcgtg
     1501 accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc cttaaaaaag
     1561 ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct gaagaggact
     1621 tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg aactcttgtg
     1681 cgtaaggaaa agtaaggaaa acgattcctt ctaacagaaa tgtcctgagc aatcacctat
     1741 gaactgtcga ctcgagcata gcatttttat ccataagatt agcggatcct aagctttaca
     1801 attgtgagcg ctcacaatta tgatagattc aattgtgagc ggataacaat ttcacacagc
     1861 aaaaaaagca aaaagcattt accataggtc ccaatataag gagagggtgt acaatgagca
     1921 caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt
     1981 atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga
     2041 tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg
     2101 ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca
     2161 gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg
     2221 agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta
     2281 ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct
     2341 ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg
     2401 acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag
     2461 ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt
     2521 ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg
     2581 tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggctgaccc cgggctgcag
     2641 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccagctt
     2701 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     2761 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2821 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2881 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2941 gagaggcggt ttgcgtattg ggcgcatgca taaaaactgt tgtaattcat taagcattct
     3001 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     3061 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     3121 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     3181 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     3241 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     3301 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     3361 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     3421 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     3481 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     3541 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     3601 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     3661 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
     3721 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     3781 tgcgaagtga tcttccgtca caggtattta ttcgaagacg aaagggcctc gtgatacgcc
     3841 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     3901 ggggaaatgt gcgcgcccgc gttcctgctg gcgctgggcc tgtttctggc gctggacttc
     3961 ccgctgttcc gtcagcagct tttcgcccac ggccttgatg atcgcggcgg ccttggcctg
     4021 catatcccga ttcaacggcc ccagggcgtc cagaacgggc ttcaggcgct cccgaaggtc
     4081 tcgggccgtc tcttgggctt gatcggcctt cttgcgcatc tcacgcgctc ctgcggcggc
     4141 ctgtagggca ggctcatacc cctgccgaac cgcttttgtc agccggtcgg ccacggcttc
     4201 cggcgtctca acgcgctttg agattcccag cttttcggcc aatccctgcg gtgcataggc
     4261 gcgtggctcg accgcttgcg ggctgatggt gacgtggccc actggtggcc gctccagggc
     4321 ctcgtagaac gcctgaatgc gcgtgtgacg tgccttgctg ccctcgatgc cccgttgcag
     4381 ccctagatcg gccacagcgg ccgcaaacgt ggtctggtcg cgggtcatct gcgctttgtt
     4441 gccgatgaac tccttggccg acagcctgcc gtcctgcgtc agcggcacca cgaacgcggt
     4501 catgtgcggg ctggtttcgt cacggtggat gctggccgtc acgatgcgat ccgccccgta
     4561 cttgtccgcc agccacttgt gcgccttctc gaagaacgcc gcctgctgtt cttggctggc
     4621 cgacttccac cattccgggc tggccgtcat gacgtactcg accgccaaca cagcgtcctt
     4681 gcgccgcttc tctggcagca actcgcgcag tcggcccatc gcttcatcgg tgctgctggc
     4741 cgcccagtgc tcgttctctg gcgtcctgct ggcgtcagcg ttgggcgtct cgcgctcgcg
     4801 gtaggcgtgc ttgagactgg ccgccacgtt gcccattttc gccagcttct tgcatcgcat
     4861 gatcgcgtat gccgccatgc ctgcccctcc cttttggtgt ccaaccggct cgacgggggc
     4921 agcgcaaggc ggtgcctccg gcgggccact caatgcttga gtatactcac tagactttgc
     4981 ttcgcaaagt cgtgaccgcc tacggcggct gcggcgccct acgggcttgc tctccgggct
     5041 tcgccctgcg cggtcgctgc gctcccttgc cagcccgtgg atatgtggac gatggccgcg
     5101 agcggccacc ggctggctcg cttcgctcgg cccgtggaca accctgctgg acaagctgat
     5161 ggacaggctg cgcctgccca cgagcttgac cacagggatt gcccaccggc tacccagcct
     5221 tcgaccacat acccaccggc tccaactgcg cggcctgcgg ccttgcccca tcaatttttt
     5281 taattttctc tggggaaaag cctccggcct gcggcctgcg cgcttcgctt gccggttgga
     5341 caccaagtgg aaggcgggtc aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg
     5401 cgcctggaac gacccaagcc tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct
     5461 gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc
     5521 aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg
     5581 ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga
     5641 actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac
     5701 gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt
     5761 aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct
     5821 gattgcgcat ggtgccgcaa ccgtgcggca ccctaccgca tggagataag catggccacg
     5881 cagtccagag aaatcggcat tcaagccaag aacaagcccg gtcactgggt gcaaacggaa
     5941 cgcaaagcgc atgaggcgtg ggccgggctt attgcgagga aacccacggc ggcaatgctg
     6001 ctgcatcacc tcgtggcgca gatgggccac cagaacgccg tggtggtcag ccagaagaca
     6061 ctttccaagc tcatcggacg ttctttgcgg acggtccaat acgcagtcaa ggacttggtg
     6121 gccgagcgct ggatctccgt cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg
     6181 gtcaatgacc gcgtggcgtg gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt
     6241 gccgccgtgg tggttgatca cgacgaccag gacgaatcgc tgttggggca tggcgacctg
     6301 cgccgcatcc cgaccctgta tccgggcgag cagcaactac cgaccggccc cggcgaggag
     6361 ccgcccagcc agcccggcat tccgggcatg gaaccagacc tgccagcctt gaccgaaacg
     6421 gaggaatggg aacggcgcgg gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg
     6481 gacgatggcg agccgttgga gccgccgaca cgggtcacgc tgccgcgccg gtagcacttg
     6541 ggttgcgcag caacccgtaa gtgcgctgtt ccagactatc ggctgtagcc gcctcgccgc
     6601 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     6661 ctgaatggaa gccggcggca cctcgctaac ggattcaccg tttttatcag gctctgggag
     6721 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
     6781 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     6841 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat
     6901 ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg
     6961 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     7021 gtcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     7081 aatattaacg c
//
