LOCUS       Exported                7575 bp ds-DNA     circular UNA 21-NOV-2016
DEFINITION  pOP576 with gbOP69 cloned in at SacI.
ACCESSION   urn.local...1479743665943.34
VERSION     .
KEYWORDS    pOP589
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        12..155
                     /label=proD
                     /note="proD promoter"
     primer_bind     37..60
                     /label=OP598
                     /note="OP598"
     misc_feature    complement(124..155)
                     /label=mmD41
                     /note="mmD41"
                     /note="Geneious type: primer_bind_reverse"
     misc_RNA        156..206
                     /label=RBS
                     /note="RBS"
     primer_bind     156..181
                     /label=OP245
                     /note="OP245"
     primer_bind     156..180
                     /label=OP283
                     /note="OP283"
     misc_feature    complement(181..206)
                     /label=OP290
                     /note="OP290"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(181..206)
                     /label=OP640
                     /note="OP640"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     181..206
                     /label=OP305
                     /note="OP305"
     CDS             207..4313
                     /label=dCas9
                     /note="dCas9"
     primer_bind     4248..4275
                     /label=OP1077
                     /note="OP1077"
     promoter        4326..4360
                     /label=BBa_J23119
                     /note="BBa_J23119 promoter"
     misc_RNA        4364..4388
                     /label=guide
                     /note="guide"
     misc_RNA        4389..4470
                     /label=Handle
                     /note="Handle"
     terminator      4471..4575
                     /label=T1 terminator
                     /note="T1 terminator"
     CDS             complement(4742..5692)
                     /label=repA101
                     /note="repA101"
     primer_bind     6439..6459
                     /label=OP363
                     /note="OP363"
     primer_bind     6445..6469
                     /label=pL131-f10
                     /note="pL131-f10"
     CDS             complement(6584..7444)
                     /label=ampR
                     /note="ampR"
     primer_bind     6584..6606
                     /label=TEM3-BamHI-R
                     /note="TEM3-BamHI-R"
     primer_bind     6584..6606
                     /label=TEM3-KIlacZYA-R
                     /note="TEM3-KIlacZYA-R"
     primer_bind     6626..6652
                     /label=OP514
                     /note="OP514"
     misc_feature    complement(6671..6690)
                     /label=amp-3-F
                     /note="amp-3-F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6886..6909
                     /label=JP111
                     /note="JP111"
     primer_bind     6949..6970
                     /label=pL131-f11
                     /note="pL131-f11"
     primer_bind     7316..7336
                     /label=JP112
                     /note="JP112"
     primer_bind     7316..7336
                     /label=OP614
                     /note="OP614"
     primer_bind     7408..7431
                     /label=pL037-bp4274-bp4297-f (D1160)
                     /note="pL037-bp4274-bp4297-f (D1160)"
     primer_bind     7408..7428
                     /label=pL131-f12
                     /note="pL131-f12"
     primer_bind     7543..7562
                     /label=Carb_F
                     /note="Carb_F"
     misc_feature    complement(7560..10)
                     /label=OP1088 binding region
                     /note="OP1088 binding region"
ORIGIN
        1 catcgattta gcacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg
       61 gttgctggat aactttacgg gcatgcataa ggctcgtata atatattcag ggagaccaca
      121 acggtttccc tctacaaata attttgttta actttgcatg ccagttgtag atcagcatag
      181 catagacaac caaggaggta gtgcacatgg ataagaaata ctctattggt ttggctatcg
      241 gtacaaactc tgttggttgg gctgttatta ctgatgaata caaggttcca tccaagaagt
      301 tcaaggtttt gggtaacact gatagacact ccatcaaaaa gaacttgatt ggtgccttgt
      361 tgttcgattc tggtgaaact gctgaagcta ctagattgaa aagaaccgct agaagaagat
      421 acaccagaag aaagaacaga atctgctact tgcaagaaat cttctccaac gaaatggcca
      481 aggttgatga ttcattcttc cacagattgg aagaatcctt cttggtcgaa gaagataaga
      541 agcacgaaag acatccaatc ttcggtaaca tcgttgatga agttgcttac cacgaaaagt
      601 acccaactat ctaccatttg agaaagaagt tggttgactc taccgataag gctgatttga
      661 gattgatcta tttggctttg gcccacatga ttaagttcag aggtcatttc ttgatcgaag
      721 gtgatttgaa cccagataac tccgatgttg ataagttgtt catccaatta gtccaaacct
      781 acaatcaatt attcgaagaa aacccaatca acgcctctgg tgttgatgct aaagctattt
      841 tgtctgccag attgtccaag tccagaagat tagaaaattt gatcgcccaa ttaccaggtg
      901 aaaagaagaa tggtttgttc ggtaatttga ttgccttgtc tttgggtttg actccaaact
      961 tcaagtccaa tttcgatttg gctgaagatg ccaagttgca attatctaag gatacctacg
     1021 atgacgattt ggataacttg ttggctcaaa tcggtgatca atacgctgat ttgtttttgg
     1081 ctgctaagaa cttgtccgat gccattttgt tgtccgatat tttgagagtc aacaccgaaa
     1141 ttactaaggc tccattgtct gcctctatga tcaaaagata cgatgaacac caccaagact
     1201 tgactttgtt gaaggctttg gtcagacaac aattacctga aaagtacaaa gaaattttct
     1261 tcgatcaatc caagaacggt tacgccggtt atattgatgg tggtgcttct caagaagaat
     1321 tttacaagtt catcaagcca atcttggaaa agatggacgg tactgaagaa ttattggtca
     1381 agttgaacag agaagatttg ttgagaaagc aaagaacctt cgacaacggt tctattccac
     1441 atcaaattca cttgggtgaa ttgcacgcaa ttttgagaag acaagaagat ttttatccat
     1501 tcttgaagga caacagagaa aagatcgaaa agattctgac cttcagaatc ccttactacg
     1561 ttggtccatt ggctagaggt aattcaagat ttgcctggat gactagaaag tccgaagaaa
     1621 ctattactcc ttggaacttc gaagaagttg tagataaggg tgcttctgcc caatccttta
     1681 ttgaaagaat gaccaacttc gacaagaact tgccaaacga aaaggttttg ccaaagcact
     1741 ctttgttgta cgaatacttc accgtctaca acgaattgac taaggttaag tacgtcaccg
     1801 aaggtatgag aaaaccagct tttttatccg gtgaacaaaa gaaggctatc gtcgatttgt
     1861 tgttcaagac caacagaaag gttactgtca agcaattaaa agaagattac ttcaagaaaa
     1921 tcgaatgctt cgactccgtt gaaatttctg gtgtcgaaga tagattcaat gcctctttag
     1981 gtacttacca tgacttgttg aaaatcatca aggacaagga tttcttggac aacgaagaaa
     2041 acgaagatat tttggaagat attgtcttga cattgacctt gtttgaagat agagaaatga
     2101 ttgaagaaag attgaaaacc tacgcccact tgttcgatga taaggttatg aagcaattaa
     2161 agagaagaag atacactggt tggggtagat tgtccagaaa attgattaac ggtatcagag
     2221 acaagcaatc cggtaagacc attttggact ttttgaagtc tgatggtttc gctaacagaa
     2281 acttcatgca attaatccac gacgattcct tgactttcaa agaagatata caaaaggccc
     2341 aagtctctgg tcaaggtgat tctttacatg aacatatcgc taacttggct ggttctccag
     2401 ctattaagaa gggtatttta caaaccgtta aggtcgttga cgaattggtc aaagttatgg
     2461 gtagacataa gccagaaaac atcgttatcg aaatggctag agaaaatcaa accacccaaa
     2521 agggtcaaaa gaactccaga gaaagaatga agagaatcga agaaggtatc aaagaattgg
     2581 gttcccaaat tttgaaagaa cacccagttg aaaacaccca attacaaaac gaaaagttgt
     2641 acttgtacta cttgcaaaac ggtagagata tgtacgttga ccaagaattg gacatcaaca
     2701 gattgtctga ttacgatgtt gacgctatcg ttccacaatc ttttttgaag gatgactcca
     2761 ttgacaacaa ggtcttgact agatccgata agaatagagg taagtccgat aacgttccat
     2821 ctgaagaagt cgttaagaaa atgaagaact attggagaca attattgaac gccaagttga
     2881 tcacccaaag aaagtttgac aatttgacca aggctgaaag aggtggtttg tctgaattgg
     2941 ataaggcagg tttcatcaaa agacaattag tagaaaccag acaaatcacc aagcacgttg
     3001 ctcaaatttt ggatagtaga atgaacacta agtacgacga aaacgacaaa ttgatcagag
     3061 aagttaaggt cattaccttg aagtccaagt tggtttccga tttcagaaag gacttccaat
     3121 tctacaaggt cagagaaatc aacaactacc atcatgcaca tgatgcttac ttgaatgctg
     3181 ttgttggtac tgccttgatt aagaagtatc caaagttgga atccgaattt gtctacggtg
     3241 attacaaggt ttacgacgtt agaaagatga tcgccaagtc cgaacaagaa attggtaaag
     3301 ctactgccaa atacttcttc tactccaata ttatgaattt ctttaagacc gaaatcactt
     3361 tggccaacgg tgaaattaga aaaagaccat tgattgaaac taatggtgaa acaggtgaaa
     3421 tcgtttggga taagggtaga gattttgcca ctgttagaaa ggtattgtcc atgccacaag
     3481 taaacatcgt caaaaagacc gaagttcaaa ctggtggttt ctccaaagaa tccattttgc
     3541 ctaagagaaa ctccgataag ttgatcgcta gaaaaaaaga ctgggaccca aaaaagtacg
     3601 gtggttttga ttctccaact gttgcttact ctgttttggt tgttgctaag gtcgaaaagg
     3661 gtaagagtaa gaagttgaag tccgtcaaag aattattagg tatcactatc atggaaagat
     3721 cctcattcga aaagaatcct atcgactttt tggaagccaa gggttacaaa gaagtcaaga
     3781 aggacttgat cattaagttg ccaaagtaca gtttgttcga attggaaaat ggtagaaaga
     3841 gaatgttggc ttctgccggt gaattacaaa agggtaatga attggctttg ccatccaagt
     3901 acgttaattt cttatacttg gcctcccact acgaaaaatt gaaaggttct cctgaagata
     3961 acgaacaaaa gcaattattt gtcgaacaac acaagcacta cttggacgaa atcattgaac
     4021 aaatttccga attttccaaa agagtcattt tggctgacgc caatttggac aaagttttgt
     4081 cagcttacaa caagcacaga gataagccaa ttagagaaca agctgaaaac atcattcact
     4141 tgttcacttt gactaacttg ggtgctccag ctgcttttaa gtatttcgat accactatcg
     4201 acagaaagag atacacctct accaaagaag ttttggacgc tactttgatc caccaatcta
     4261 ttactggttt gtacgaaact agaatcgact tgtctcaatt aggtggtgat taacaagctt
     4321 gagctttgac agctagctca gtcctaggta taatgctagc cccttaatca tccggctcgt
     4381 ataatgtggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     4441 gaaaaagtgg caccgagtcg gtgctttttt ggcatcaaat aaaacgaaag gctcagtcga
     4501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     4561 atccgccgcc ctagaagctc gaattctcta gagaatcagg tttgtgccaa taccagtaga
     4621 aacagacgaa gaatccatgg gtatggacag ttttcccttt gatatgtaac ggtgaacagt
     4681 tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac
     4741 ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga
     4801 gccatgagaa cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct
     4861 caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt
     4921 tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct
     4981 ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca
     5041 acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta
     5101 aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat
     5161 tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag
     5221 ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt
     5281 caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag
     5341 gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg
     5401 tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca
     5461 tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca
     5521 tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa
     5581 tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt
     5641 catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca
     5701 attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac
     5761 tagtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac
     5821 ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt
     5881 atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag
     5941 atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt
     6001 cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca
     6061 ccctcgcaag ctcggttgcg gccgcaatcg ggcaaatcgc tgaatattcc ttttgtctcc
     6121 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
     6181 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
     6241 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
     6301 gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt
     6361 ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg
     6421 cagcggtatc atcaacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7201 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7561 gaaaagtgcc acctg
//
