LOCUS       Exported                8118 bp ds-DNA     circular UNA 21-NOV-2016
DEFINITION  pOP576 with gbOP67, gbOP68 and gbOP57 cloned in at SacI.
ACCESSION   urn.local...1479743666100.37
VERSION     .
KEYWORDS    pOP592
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        12..155
                     /label=proD
                     /note="proD promoter"
     primer_bind     37..60
                     /label=OP598
                     /note="OP598"
     misc_feature    complement(124..155)
                     /label=mmD41
                     /note="mmD41"
                     /note="Geneious type: primer_bind_reverse"
     misc_RNA        156..206
                     /label=RBS
                     /note="RBS"
     primer_bind     156..181
                     /label=OP245
                     /note="OP245"
     primer_bind     156..180
                     /label=OP283
                     /note="OP283"
     misc_feature    complement(181..206)
                     /label=OP290
                     /note="OP290"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(181..206)
                     /label=OP640
                     /note="OP640"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     181..206
                     /label=OP305
                     /note="OP305"
     CDS             207..4313
                     /label=dCas9
                     /note="dCas9"
     primer_bind     4248..4275
                     /label=OP1077
                     /note="OP1077"
     promoter        4326..4360
                     /label=BBa_J23119
                     /note="BBa_J23119 promoter"
     misc_RNA        4364..4388
                     /label=guide
                     /note="guide"
     misc_RNA        4389..4470
                     /label=Handle
                     /note="Handle"
     terminator      4471..4575
                     /label=T1 terminator
                     /note="T1 terminator"
     promoter        4597..4631
                     /label=BBa_J23119
                     /note="BBa_J23119 promoter"
     misc_RNA        4635..4659
                     /label=guide
                     /note="guide"
     misc_RNA        4660..4741
                     /label=Handle
                     /note="Handle"
     terminator      4742..4846
                     /label=T1 terminator
                     /note="T1 terminator"
     modified_base   4868
                     /label=poss deletion
                     /note="poss deletion"
     promoter        4869..4903
                     /label=BBa_J23119
                     /note="BBa_J23119 promoter"
     misc_RNA        4907..4931
                     /label=guide
                     /note="guide"
     misc_RNA        4932..5013
                     /label=Handle
                     /note="Handle"
     terminator      5014..5118
                     /label=T1 terminator
                     /note="T1 terminator"
     CDS             complement(5285..6235)
                     /label=repA101
                     /note="repA101"
     primer_bind     6982..7002
                     /label=OP363
                     /note="OP363"
     primer_bind     6988..7012
                     /label=pL131-f10
                     /note="pL131-f10"
     CDS             complement(7127..7987)
                     /label=ampR
                     /note="ampR"
     primer_bind     7127..7149
                     /label=TEM3-BamHI-R
                     /note="TEM3-BamHI-R"
     primer_bind     7127..7149
                     /label=TEM3-KIlacZYA-R
                     /note="TEM3-KIlacZYA-R"
     primer_bind     7169..7195
                     /label=OP514
                     /note="OP514"
     misc_feature    complement(7214..7233)
                     /label=amp-3-F
                     /note="amp-3-F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     7429..7452
                     /label=JP111
                     /note="JP111"
     primer_bind     7492..7513
                     /label=pL131-f11
                     /note="pL131-f11"
     primer_bind     7859..7879
                     /label=JP112
                     /note="JP112"
     primer_bind     7859..7879
                     /label=OP614
                     /note="OP614"
     primer_bind     7951..7974
                     /label=pL037-bp4274-bp4297-f (D1160)
                     /note="pL037-bp4274-bp4297-f (D1160)"
     primer_bind     7951..7971
                     /label=pL131-f12
                     /note="pL131-f12"
     primer_bind     8086..8105
                     /label=Carb_F
                     /note="Carb_F"
     misc_feature    complement(8103..10)
                     /label=OP1088 binding region
                     /note="OP1088 binding region"
ORIGIN
        1 catcgattta gcacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg
       61 gttgctggat aactttacgg gcatgcataa ggctcgtata atatattcag ggagaccaca
      121 acggtttccc tctacaaata attttgttta actttgcatg ccagttgtag atcagcatag
      181 catagacaac caaggaggta gtgcacatgg ataagaaata ctctattggt ttggctatcg
      241 gtacaaactc tgttggttgg gctgttatta ctgatgaata caaggttcca tccaagaagt
      301 tcaaggtttt gggtaacact gatagacact ccatcaaaaa gaacttgatt ggtgccttgt
      361 tgttcgattc tggtgaaact gctgaagcta ctagattgaa aagaaccgct agaagaagat
      421 acaccagaag aaagaacaga atctgctact tgcaagaaat cttctccaac gaaatggcca
      481 aggttgatga ttcattcttc cacagattgg aagaatcctt cttggtcgaa gaagataaga
      541 agcacgaaag acatccaatc ttcggtaaca tcgttgatga agttgcttac cacgaaaagt
      601 acccaactat ctaccatttg agaaagaagt tggttgactc taccgataag gctgatttga
      661 gattgatcta tttggctttg gcccacatga ttaagttcag aggtcatttc ttgatcgaag
      721 gtgatttgaa cccagataac tccgatgttg ataagttgtt catccaatta gtccaaacct
      781 acaatcaatt attcgaagaa aacccaatca acgcctctgg tgttgatgct aaagctattt
      841 tgtctgccag attgtccaag tccagaagat tagaaaattt gatcgcccaa ttaccaggtg
      901 aaaagaagaa tggtttgttc ggtaatttga ttgccttgtc tttgggtttg actccaaact
      961 tcaagtccaa tttcgatttg gctgaagatg ccaagttgca attatctaag gatacctacg
     1021 atgacgattt ggataacttg ttggctcaaa tcggtgatca atacgctgat ttgtttttgg
     1081 ctgctaagaa cttgtccgat gccattttgt tgtccgatat tttgagagtc aacaccgaaa
     1141 ttactaaggc tccattgtct gcctctatga tcaaaagata cgatgaacac caccaagact
     1201 tgactttgtt gaaggctttg gtcagacaac aattacctga aaagtacaaa gaaattttct
     1261 tcgatcaatc caagaacggt tacgccggtt atattgatgg tggtgcttct caagaagaat
     1321 tttacaagtt catcaagcca atcttggaaa agatggacgg tactgaagaa ttattggtca
     1381 agttgaacag agaagatttg ttgagaaagc aaagaacctt cgacaacggt tctattccac
     1441 atcaaattca cttgggtgaa ttgcacgcaa ttttgagaag acaagaagat ttttatccat
     1501 tcttgaagga caacagagaa aagatcgaaa agattctgac cttcagaatc ccttactacg
     1561 ttggtccatt ggctagaggt aattcaagat ttgcctggat gactagaaag tccgaagaaa
     1621 ctattactcc ttggaacttc gaagaagttg tagataaggg tgcttctgcc caatccttta
     1681 ttgaaagaat gaccaacttc gacaagaact tgccaaacga aaaggttttg ccaaagcact
     1741 ctttgttgta cgaatacttc accgtctaca acgaattgac taaggttaag tacgtcaccg
     1801 aaggtatgag aaaaccagct tttttatccg gtgaacaaaa gaaggctatc gtcgatttgt
     1861 tgttcaagac caacagaaag gttactgtca agcaattaaa agaagattac ttcaagaaaa
     1921 tcgaatgctt cgactccgtt gaaatttctg gtgtcgaaga tagattcaat gcctctttag
     1981 gtacttacca tgacttgttg aaaatcatca aggacaagga tttcttggac aacgaagaaa
     2041 acgaagatat tttggaagat attgtcttga cattgacctt gtttgaagat agagaaatga
     2101 ttgaagaaag attgaaaacc tacgcccact tgttcgatga taaggttatg aagcaattaa
     2161 agagaagaag atacactggt tggggtagat tgtccagaaa attgattaac ggtatcagag
     2221 acaagcaatc cggtaagacc attttggact ttttgaagtc tgatggtttc gctaacagaa
     2281 acttcatgca attaatccac gacgattcct tgactttcaa agaagatata caaaaggccc
     2341 aagtctctgg tcaaggtgat tctttacatg aacatatcgc taacttggct ggttctccag
     2401 ctattaagaa gggtatttta caaaccgtta aggtcgttga cgaattggtc aaagttatgg
     2461 gtagacataa gccagaaaac atcgttatcg aaatggctag agaaaatcaa accacccaaa
     2521 agggtcaaaa gaactccaga gaaagaatga agagaatcga agaaggtatc aaagaattgg
     2581 gttcccaaat tttgaaagaa cacccagttg aaaacaccca attacaaaac gaaaagttgt
     2641 acttgtacta cttgcaaaac ggtagagata tgtacgttga ccaagaattg gacatcaaca
     2701 gattgtctga ttacgatgtt gacgctatcg ttccacaatc ttttttgaag gatgactcca
     2761 ttgacaacaa ggtcttgact agatccgata agaatagagg taagtccgat aacgttccat
     2821 ctgaagaagt cgttaagaaa atgaagaact attggagaca attattgaac gccaagttga
     2881 tcacccaaag aaagtttgac aatttgacca aggctgaaag aggtggtttg tctgaattgg
     2941 ataaggcagg tttcatcaaa agacaattag tagaaaccag acaaatcacc aagcacgttg
     3001 ctcaaatttt ggatagtaga atgaacacta agtacgacga aaacgacaaa ttgatcagag
     3061 aagttaaggt cattaccttg aagtccaagt tggtttccga tttcagaaag gacttccaat
     3121 tctacaaggt cagagaaatc aacaactacc atcatgcaca tgatgcttac ttgaatgctg
     3181 ttgttggtac tgccttgatt aagaagtatc caaagttgga atccgaattt gtctacggtg
     3241 attacaaggt ttacgacgtt agaaagatga tcgccaagtc cgaacaagaa attggtaaag
     3301 ctactgccaa atacttcttc tactccaata ttatgaattt ctttaagacc gaaatcactt
     3361 tggccaacgg tgaaattaga aaaagaccat tgattgaaac taatggtgaa acaggtgaaa
     3421 tcgtttggga taagggtaga gattttgcca ctgttagaaa ggtattgtcc atgccacaag
     3481 taaacatcgt caaaaagacc gaagttcaaa ctggtggttt ctccaaagaa tccattttgc
     3541 ctaagagaaa ctccgataag ttgatcgcta gaaaaaaaga ctgggaccca aaaaagtacg
     3601 gtggttttga ttctccaact gttgcttact ctgttttggt tgttgctaag gtcgaaaagg
     3661 gtaagagtaa gaagttgaag tccgtcaaag aattattagg tatcactatc atggaaagat
     3721 cctcattcga aaagaatcct atcgactttt tggaagccaa gggttacaaa gaagtcaaga
     3781 aggacttgat cattaagttg ccaaagtaca gtttgttcga attggaaaat ggtagaaaga
     3841 gaatgttggc ttctgccggt gaattacaaa agggtaatga attggctttg ccatccaagt
     3901 acgttaattt cttatacttg gcctcccact acgaaaaatt gaaaggttct cctgaagata
     3961 acgaacaaaa gcaattattt gtcgaacaac acaagcacta cttggacgaa atcattgaac
     4021 aaatttccga attttccaaa agagtcattt tggctgacgc caatttggac aaagttttgt
     4081 cagcttacaa caagcacaga gataagccaa ttagagaaca agctgaaaac atcattcact
     4141 tgttcacttt gactaacttg ggtgctccag ctgcttttaa gtatttcgat accactatcg
     4201 acagaaagag atacacctct accaaagaag ttttggacgc tactttgatc caccaatcta
     4261 ttactggttt gtacgaaact agaatcgact tgtctcaatt aggtggtgat taacaagctt
     4321 gagctttgac agctagctca gtcctaggta taatgctagc ccctgataga gatactgagc
     4381 acatcagcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     4441 gaaaaagtgg caccgagtcg gtgctttttt ggcatcaaat aaaacgaaag gctcagtcga
     4501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     4561 atccgccgcc ctagaatccc tagtcgatta gcacctttga cagctagctc agtcctaggt
     4621 ataatgctag cccccaatta tgatagattc aattgtgagg ttttagagct agaaatagca
     4681 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4741 tggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4801 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagacctc gataccctag
     4861 ctaatacatt gacagctagc tcagtcctag gtataatgct agccccaaac cattaacaca
     4921 agagcagctt ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     4981 cttgaaaaag tggcaccgag tcggtgcttt tttggcatca aataaaacga aaggctcagt
     5041 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     5101 caaatccgcc gccctagaag ctcgaattct ctagagaatc aggtttgtgc caataccagt
     5161 agaaacagac gaagaatcca tgggtatgga cagttttccc tttgatatgt aacggtgaac
     5221 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     5281 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     5341 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     5401 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     5461 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     5521 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     5581 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     5641 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     5701 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     5761 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     5821 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     5881 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     5941 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     6001 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     6061 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     6121 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     6181 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     6241 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     6301 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     6361 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     6421 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     6481 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     6541 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     6601 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc
     6661 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     6721 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     6781 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     6841 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     6901 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     6961 aggcagcggt atcatcaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8101 cccgaaaagt gccacctg
//
