LOCUS       Exported                7846 bp ds-DNA     circular UNA 21-NOV-2016
DEFINITION  pOP576 with gbOP66 and gbOP68 cloned in at SacI.
ACCESSION   urn.local...1479743666208.39
VERSION     .
KEYWORDS    pOP594
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        12..155
                     /label=proD
                     /note="proD promoter"
     primer_bind     37..60
                     /label=OP598
                     /note="OP598"
     misc_feature    complement(124..155)
                     /label=mmD41
                     /note="mmD41"
                     /note="Geneious type: primer_bind_reverse"
     misc_RNA        156..206
                     /label=RBS
                     /note="RBS"
     primer_bind     156..181
                     /label=OP245
                     /note="OP245"
     primer_bind     156..180
                     /label=OP283
                     /note="OP283"
     misc_feature    complement(181..206)
                     /label=OP290
                     /note="OP290"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(181..206)
                     /label=OP640
                     /note="OP640"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     181..206
                     /label=OP305
                     /note="OP305"
     CDS             207..4313
                     /label=dCas9
                     /note="dCas9"
     primer_bind     4248..4275
                     /label=OP1077
                     /note="OP1077"
     promoter        4326..4360
                     /label=BBa_J23119
                     /note="BBa_J23119 promoter"
     misc_RNA        4364..4388
                     /label=guide
                     /note="guide"
     misc_RNA        4389..4470
                     /label=Handle
                     /note="Handle"
     terminator      4471..4575
                     /label=T1 terminator
                     /note="T1 terminator"
     promoter        4597..4631
                     /label=BBa_J23119
                     /note="BBa_J23119 promoter"
     misc_RNA        4635..4659
                     /label=guide
                     /note="guide"
     misc_RNA        4660..4741
                     /label=Handle
                     /note="Handle"
     terminator      4742..4846
                     /label=T1 terminator
                     /note="T1 terminator"
     CDS             complement(5013..5963)
                     /label=repA101
                     /note="repA101"
     primer_bind     6710..6730
                     /label=OP363
                     /note="OP363"
     primer_bind     6716..6740
                     /label=pL131-f10
                     /note="pL131-f10"
     CDS             complement(6855..7715)
                     /label=ampR
                     /note="ampR"
     primer_bind     6855..6877
                     /label=TEM3-BamHI-R
                     /note="TEM3-BamHI-R"
     primer_bind     6855..6877
                     /label=TEM3-KIlacZYA-R
                     /note="TEM3-KIlacZYA-R"
     primer_bind     6897..6923
                     /label=OP514
                     /note="OP514"
     misc_feature    complement(6942..6961)
                     /label=amp-3-F
                     /note="amp-3-F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     7157..7180
                     /label=JP111
                     /note="JP111"
     primer_bind     7220..7241
                     /label=pL131-f11
                     /note="pL131-f11"
     primer_bind     7587..7607
                     /label=JP112
                     /note="JP112"
     primer_bind     7587..7607
                     /label=OP614
                     /note="OP614"
     primer_bind     7679..7702
                     /label=pL037-bp4274-bp4297-f (D1160)
                     /note="pL037-bp4274-bp4297-f (D1160)"
     primer_bind     7679..7699
                     /label=pL131-f12
                     /note="pL131-f12"
     primer_bind     7814..7833
                     /label=Carb_F
                     /note="Carb_F"
     misc_feature    complement(7831..10)
                     /label=OP1088 binding region
                     /note="OP1088 binding region"
ORIGIN
        1 catcgattta gcacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg
       61 gttgctggat aactttacgg gcatgcataa ggctcgtata atatattcag ggagaccaca
      121 acggtttccc tctacaaata attttgttta actttgcatg ccagttgtag atcagcatag
      181 catagacaac caaggaggta gtgcacatgg ataagaaata ctctattggt ttggctatcg
      241 gtacaaactc tgttggttgg gctgttatta ctgatgaata caaggttcca tccaagaagt
      301 tcaaggtttt gggtaacact gatagacact ccatcaaaaa gaacttgatt ggtgccttgt
      361 tgttcgattc tggtgaaact gctgaagcta ctagattgaa aagaaccgct agaagaagat
      421 acaccagaag aaagaacaga atctgctact tgcaagaaat cttctccaac gaaatggcca
      481 aggttgatga ttcattcttc cacagattgg aagaatcctt cttggtcgaa gaagataaga
      541 agcacgaaag acatccaatc ttcggtaaca tcgttgatga agttgcttac cacgaaaagt
      601 acccaactat ctaccatttg agaaagaagt tggttgactc taccgataag gctgatttga
      661 gattgatcta tttggctttg gcccacatga ttaagttcag aggtcatttc ttgatcgaag
      721 gtgatttgaa cccagataac tccgatgttg ataagttgtt catccaatta gtccaaacct
      781 acaatcaatt attcgaagaa aacccaatca acgcctctgg tgttgatgct aaagctattt
      841 tgtctgccag attgtccaag tccagaagat tagaaaattt gatcgcccaa ttaccaggtg
      901 aaaagaagaa tggtttgttc ggtaatttga ttgccttgtc tttgggtttg actccaaact
      961 tcaagtccaa tttcgatttg gctgaagatg ccaagttgca attatctaag gatacctacg
     1021 atgacgattt ggataacttg ttggctcaaa tcggtgatca atacgctgat ttgtttttgg
     1081 ctgctaagaa cttgtccgat gccattttgt tgtccgatat tttgagagtc aacaccgaaa
     1141 ttactaaggc tccattgtct gcctctatga tcaaaagata cgatgaacac caccaagact
     1201 tgactttgtt gaaggctttg gtcagacaac aattacctga aaagtacaaa gaaattttct
     1261 tcgatcaatc caagaacggt tacgccggtt atattgatgg tggtgcttct caagaagaat
     1321 tttacaagtt catcaagcca atcttggaaa agatggacgg tactgaagaa ttattggtca
     1381 agttgaacag agaagatttg ttgagaaagc aaagaacctt cgacaacggt tctattccac
     1441 atcaaattca cttgggtgaa ttgcacgcaa ttttgagaag acaagaagat ttttatccat
     1501 tcttgaagga caacagagaa aagatcgaaa agattctgac cttcagaatc ccttactacg
     1561 ttggtccatt ggctagaggt aattcaagat ttgcctggat gactagaaag tccgaagaaa
     1621 ctattactcc ttggaacttc gaagaagttg tagataaggg tgcttctgcc caatccttta
     1681 ttgaaagaat gaccaacttc gacaagaact tgccaaacga aaaggttttg ccaaagcact
     1741 ctttgttgta cgaatacttc accgtctaca acgaattgac taaggttaag tacgtcaccg
     1801 aaggtatgag aaaaccagct tttttatccg gtgaacaaaa gaaggctatc gtcgatttgt
     1861 tgttcaagac caacagaaag gttactgtca agcaattaaa agaagattac ttcaagaaaa
     1921 tcgaatgctt cgactccgtt gaaatttctg gtgtcgaaga tagattcaat gcctctttag
     1981 gtacttacca tgacttgttg aaaatcatca aggacaagga tttcttggac aacgaagaaa
     2041 acgaagatat tttggaagat attgtcttga cattgacctt gtttgaagat agagaaatga
     2101 ttgaagaaag attgaaaacc tacgcccact tgttcgatga taaggttatg aagcaattaa
     2161 agagaagaag atacactggt tggggtagat tgtccagaaa attgattaac ggtatcagag
     2221 acaagcaatc cggtaagacc attttggact ttttgaagtc tgatggtttc gctaacagaa
     2281 acttcatgca attaatccac gacgattcct tgactttcaa agaagatata caaaaggccc
     2341 aagtctctgg tcaaggtgat tctttacatg aacatatcgc taacttggct ggttctccag
     2401 ctattaagaa gggtatttta caaaccgtta aggtcgttga cgaattggtc aaagttatgg
     2461 gtagacataa gccagaaaac atcgttatcg aaatggctag agaaaatcaa accacccaaa
     2521 agggtcaaaa gaactccaga gaaagaatga agagaatcga agaaggtatc aaagaattgg
     2581 gttcccaaat tttgaaagaa cacccagttg aaaacaccca attacaaaac gaaaagttgt
     2641 acttgtacta cttgcaaaac ggtagagata tgtacgttga ccaagaattg gacatcaaca
     2701 gattgtctga ttacgatgtt gacgctatcg ttccacaatc ttttttgaag gatgactcca
     2761 ttgacaacaa ggtcttgact agatccgata agaatagagg taagtccgat aacgttccat
     2821 ctgaagaagt cgttaagaaa atgaagaact attggagaca attattgaac gccaagttga
     2881 tcacccaaag aaagtttgac aatttgacca aggctgaaag aggtggtttg tctgaattgg
     2941 ataaggcagg tttcatcaaa agacaattag tagaaaccag acaaatcacc aagcacgttg
     3001 ctcaaatttt ggatagtaga atgaacacta agtacgacga aaacgacaaa ttgatcagag
     3061 aagttaaggt cattaccttg aagtccaagt tggtttccga tttcagaaag gacttccaat
     3121 tctacaaggt cagagaaatc aacaactacc atcatgcaca tgatgcttac ttgaatgctg
     3181 ttgttggtac tgccttgatt aagaagtatc caaagttgga atccgaattt gtctacggtg
     3241 attacaaggt ttacgacgtt agaaagatga tcgccaagtc cgaacaagaa attggtaaag
     3301 ctactgccaa atacttcttc tactccaata ttatgaattt ctttaagacc gaaatcactt
     3361 tggccaacgg tgaaattaga aaaagaccat tgattgaaac taatggtgaa acaggtgaaa
     3421 tcgtttggga taagggtaga gattttgcca ctgttagaaa ggtattgtcc atgccacaag
     3481 taaacatcgt caaaaagacc gaagttcaaa ctggtggttt ctccaaagaa tccattttgc
     3541 ctaagagaaa ctccgataag ttgatcgcta gaaaaaaaga ctgggaccca aaaaagtacg
     3601 gtggttttga ttctccaact gttgcttact ctgttttggt tgttgctaag gtcgaaaagg
     3661 gtaagagtaa gaagttgaag tccgtcaaag aattattagg tatcactatc atggaaagat
     3721 cctcattcga aaagaatcct atcgactttt tggaagccaa gggttacaaa gaagtcaaga
     3781 aggacttgat cattaagttg ccaaagtaca gtttgttcga attggaaaat ggtagaaaga
     3841 gaatgttggc ttctgccggt gaattacaaa agggtaatga attggctttg ccatccaagt
     3901 acgttaattt cttatacttg gcctcccact acgaaaaatt gaaaggttct cctgaagata
     3961 acgaacaaaa gcaattattt gtcgaacaac acaagcacta cttggacgaa atcattgaac
     4021 aaatttccga attttccaaa agagtcattt tggctgacgc caatttggac aaagttttgt
     4081 cagcttacaa caagcacaga gataagccaa ttagagaaca agctgaaaac atcattcact
     4141 tgttcacttt gactaacttg ggtgctccag ctgcttttaa gtatttcgat accactatcg
     4201 acagaaagag atacacctct accaaagaag ttttggacgc tactttgatc caccaatcta
     4261 ttactggttt gtacgaaact agaatcgact tgtctcaatt aggtggtgat taacaagctt
     4321 gagctttgac agctagctca gtcctaggta taatgctagc ccccgctgta cgatgtcgca
     4381 gagtatgcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     4441 gaaaaagtgg caccgagtcg gtgctttttt ggcatcaaat aaaacgaaag gctcagtcga
     4501 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     4561 atccgccgcc ctagaatccc tagtcgatta gcacctttga cagctagctc agtcctaggt
     4621 ataatgctag cccccaatta tgatagattc aattgtgagg ttttagagct agaaatagca
     4681 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4741 tggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4801 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaagct cgaattctct
     4861 agagaatcag gtttgtgcca ataccagtag aaacagacga agaatccatg ggtatggaca
     4921 gttttccctt tgatatgtaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg
     4981 cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg
     5041 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatac
     5101 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     5161 aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt
     5221 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     5281 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     5341 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     5401 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     5461 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     5521 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     5581 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     5641 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc
     5701 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     5761 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     5821 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     5881 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     5941 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     6001 caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc
     6061 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     6121 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     6181 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     6241 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     6301 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcggttgc ggccgcaatc
     6361 gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
     6421 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
     6481 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
     6541 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     6601 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     6661 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacggg gtctgacgct
     6721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6841 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6901 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6961 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7021 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7081 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7141 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7201 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7261 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7321 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7381 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7441 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7501 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7561 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7621 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7681 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7741 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7801 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
