LOCUS Exported 14556 bp ds-DNA circular SYN 17-JAN-2016 DEFINITION . ACCESSION . VERSION . KEYWORDS pAGM4723_Tp_FcpCas9YFP_Urease SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14556) AUTHORS . TITLE Direct Submission JOURNAL Exported 17 Jan 2016 from SnapGene Viewer 2.8.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14556 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" CDS 1403..2470 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /note="pVS1 RepA" /note="color: #993366" /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" promoter 2938..3042 /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: RIGHT" CDS 3043..3837 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /note="color: #993366" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 4008..4596 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" promoter 4867..5846 /note="FCP promoter" /note="color: #c0c0c0" CDS 5847..6416 /codon_start=1 /gene="Streptomyces noursei nat1" /product="nourseothricin acetyltransferase" /note="NrsR" /note="confers resistance to nourseothricin" /note="color: #993366" /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT LREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL CGLDTALYDGTASDGEQALYMSMPCP" terminator 6419..6927 /note="FCP terminator" /note="color: #ffffff" promoter 7037..8016 /note="FCP promoter" /note="color: #c0c0c0" CDS 8017..12120 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Cas9" /note="generates RNA-guided double strand breaks in DNA" /note="color: #993366" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 12133..12153 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note="color: #cc99ff" /translation="PKKKRKV" CDS 12154..12873 /codon_start=1 /product="enhanced YFP" /note="EYFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 12154 .. 12156 / #993366 2: 12157 .. 12159 / #993366 / 1a 3: 12160 .. 12873 / #993366" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" terminator 12878..13378 /note="FCP terminator" /note="color: #ffffff" promoter 13403..13851 /note="U6 promoter" /note="color: #c0c0c0" misc_feature 13852..13953 /note="sgRNA" /note="color: #33cccc" promoter 13978..14426 /note="U6 promoter" /note="color: #c0c0c0" misc_feature 14427..14528 /note="sgRNA" /note="color: #33cccc" ORIGIN 1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac 61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta 121 tccgttcgtc catttgtatg tgccagccgt gcggctgcat gaaatcctgg ccggtttgtc 181 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 241 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 301 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 361 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 421 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 481 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 541 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 601 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 661 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 721 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 781 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 841 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 901 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 961 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1021 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1081 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1141 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 1201 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 1261 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 1321 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 1381 aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc 1441 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat 1501 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga 1561 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga 1621 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat 1681 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg 1741 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt 1801 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata 1861 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact 1921 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat 1981 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct 2041 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac 2101 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga 2161 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat 2221 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt 2281 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac 2341 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg 2401 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc 2461 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg 2521 aaaaagcttc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa 2581 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt 2641 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa 2701 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaacagct 2761 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc 2821 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg 2881 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggct ccgagtgcgc 2941 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 3001 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatggctaa aatgagaata 3061 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg 3121 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg 3181 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg 3241 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc 3301 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa 3361 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac 3421 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta 3481 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaggacac tccatttaaa 3541 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt 3601 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt 3661 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg 3721 tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg 3781 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagctg 3841 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 3901 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 3961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4621 cggttcctgc tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc 4681 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat 4741 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt 4801 aatgtactgg ggttgaacac tctgtgccga attcggatcc ggagctcgag gtcgacggta 4861 tcgataagct tgcgcttttt ccgagaactc cccataagtc aacggctcca atcaagaatg 4921 tatccgacaa cggcgagcat agcaacacgt ccgtctttgg agtagaatca tcatgttgtg 4981 gatgaataca cagatgaatg acattaaaag catgaacatg ttagagagta ggaggtagag 5041 attgatatgg tagcattgcg atgtttgttt ttggtcagca tatgatgagt ggataccaat 5101 atgatgaaag ttgaatctcg cgtttgagct cagcggtacg ttattgatcg aaagtagcct 5161 gatcaaaatc cttggagagt acaagaggat caaagaatcc agtgggggcg ataactccaa 5221 gctcgttctc aaagaggcaa tggaggtaga aactcatccc agttgagaag aagtgaaggc 5281 agtggcggtg gcgaaagcag aggcaacgag gacagacttc ctgtgggttg atgcaacgaa 5341 tatttccaga aggagaagtt tagagagttg aaccgctacc tacaatgaca aagtatcgta 5401 tcgattttga tgttggttgg ttatgaattc aaactgtaag ttggattgtg agaagatcag 5461 aagttgaacg aacacatctt tccgatcatt cacctccaca ctgcaacaac acggtacttc 5521 ttccgcggca gatctctgtc gccattctct tgtcctgttg ttggctgtga gacgaggaaa 5581 gcaacgacaa gtttcacaaa agggagttcc tttaacgaga tatgtttttt ataaagagtc 5641 ccaatagaaa gacaaattga ttcctccgtg caaacgcgca aataaacacc acgtccatta 5701 tatccatatc tttcagagta tccaacaagt gttgaaggac aggtagttga agtaacgtat 5761 cttccccctc gactggatcc atcaacaagg cgaacaaatc cattcaacct ctcataaatt 5821 atctgattta ccaaaccgat accaaaatga ccactcttga cgacacggct taccggtacc 5881 gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg 5941 acaccgtatt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg 6001 acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgccgggg 6061 aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg 6121 gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag gacatcgagg 6181 tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt 6241 tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg 6301 cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg 6361 acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc ccctgagcgc 6421 ggccgcatac tggattggtg aatcaatgag ccgtagcaca atggttacat tcggctagct 6481 aagatccaat ggcaaggacc aagtgctgga acttgttttg ctttagcaga tcttagcgtg 6541 agaggtattt gtcctctgtc aggagtagat agtagatgtt ctttttaaac taaaatgcta 6601 actgttccga attcctcatc gcagctaatc cgtacatcaa aagacaaaat gctaggtatg 6661 tgtactacat ctcctgttgc tagataagac atatgatagg aaacacacca tcaatagtca 6721 ttgtagcttt acttatacta cgcatttgca ctttcccctg agtggcagag gcgcattgag 6781 aaaatcgatc tcaacatagt ttatgtagca tcccctagat ccattacttt aagtctcctt 6841 cgtctttggt gtaggcatgt tggacacaac gaggtaaaac acaacacaaa caatgtgtcc 6901 agcaaagtag tagctgctcc agttctcccg ggggatccac tagttctaga gcggccggcc 6961 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgctgcaa 7021 gaattcaagc ttggagagct tgcgcttttt ccgagaactc cccataagtc aacggctcca 7081 atcaagaatg tatccgacaa cggcgagcat agcaacacgt ccgtctttgg agtagaatca 7141 tcatgttgtg gatgaataca cagatgaatg acattaaaag catgaacatg ttagagagta 7201 ggaggtagag attgatatgg tagcattgcg atgtttgttt ttggtcagca tatgatgagt 7261 ggataccaat atgatgaaag ttgaatctcg cgtttgagct cagcggtacg ttattgatcg 7321 aaagtagcct gatcaaaatc cttggagagt acaagaggat caaagaatcc agtgggggcg 7381 ataactccaa gctcgttctc aaagaggcaa tggaggtaga aactcatccc agttgagaag 7441 aagtgaaggc agtggcggtg gcgaaagcag aggcaacgag gacagacttc ctgtgggttg 7501 atgcaacgaa tatttccaga aggagaagtt tagagagttg aaccgctacc tacaatgaca 7561 aagtatcgta tcgattttga tgttggttgg ttatgaattc aaactgtaag ttggattgtg 7621 agaagatcag aagttgaacg aacacatctt tccgatcatt cacctccaca ctgcaacaac 7681 acggtacttc ttccgcggca gatctctgtc gccattctct tgtcctgttg ttggctgtga 7741 gacgaggaaa gcaacgacaa gtttcacaaa agggagttcc tttaacgaga tatgtttttt 7801 ataaagagtc ccaatagaaa gacaaattga ttcctccgtg caaacgcgca aataaacacc 7861 acgtccatta tatccatatc tttcagagta tccaacaagt gttgaaggac aggtagttga 7921 agtaacgtat cttccccctc gactggatcc atcaacaagg cgaacaaatc cattcaacct 7981 ctcataaatt atctgattta ccaaaccaat accaaaatgg acaagaagta ctccattggg 8041 ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg 8101 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt 8161 ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa aagaacagca 8221 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat 8281 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag 8341 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac 8401 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag 8461 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc 8521 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg 8581 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc 8641 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag 8701 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg 8761 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa 8821 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac 8881 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg 8941 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac 9001 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag 9061 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc 9121 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag 9181 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga 9241 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat 9301 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata 9361 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa 9421 tcagaagaga ctatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc 9481 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt 9541 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa 9601 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc 9661 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagattat 9721 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac 9781 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac 9841 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat 9901 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg 9961 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat 10021 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt 10081 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc 10141 cagaaagcac aagtttctgg ccagggggac agtctccacg agcacatcgc taatcttgca 10201 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc 10261 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa 10321 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata 10381 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat 10441 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg 10501 gacatcaatc ggctctccga ctacgacgtg gatcatatcg tgccccagtc ttttctcaaa 10561 gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat 10621 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac 10681 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg 10741 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc 10801 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa 10861 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtttcaga tttcagaaag 10921 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac 10981 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt 11041 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa 11101 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc 11161 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa 11221 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc 11281 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa 11341 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc 11401 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa 11461 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc 11521 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa 11581 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac 11641 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg 11701 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaaggatct 11761 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag 11821 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat 11881 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac 11941 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac 12001 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt 12061 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac 12121 agcagggctg accccaagaa gaagaggaag gtgatggtga gcaagggcga ggagctgttc 12181 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 12241 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 12301 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg 12361 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 12421 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 12481 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 12541 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 12601 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 12661 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 12721 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc 12781 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 12841 atcactctcg gcatggacga gctgtacaag tgagcttata ctggattggt gaatcaatga 12901 gccgtagcac aatggttaca ttcggctagc taagatccaa tggcaaggac caagtgctgg 12961 aacttgtttt gctttagcag atcttagcgt gagaggtatt tgtcctctgt caggagtaga 13021 tagtagatgt tctttttaaa ctaaaatgct aactgttccg aattcctcat cgcagctaat 13081 ccgtacatca aaagacaaaa tgctaggtat gtgtactaca tctcctgttg ctagataaga 13141 catatgatag gaaacacacc atcaatagtc attgtagctt tacttatact acgcatttgc 13201 actttcccct gagtggcaga ggcgcattga gaaaatcgat ctcaacatag tttatgtagc 13261 atcccctaga tccattactt taagtctcct tcgtctttgg tgtaggcatg ttggacacaa 13321 cgaggtaaaa cacaacacaa acaatgtgtc cagcaaagta gtagctgctc cagttctccg 13381 ctactagaat tcgagctcgg agcttcatca agagagcaac caacaaagaa gaggcgaaca 13441 cggtgcacag caccgtctgc atacttccga tttgcaattc acaacatatg atacatcatc 13501 catctctctg agaaaactgg gcactgttag aatctgggat ttatcaacag ccaccaacta 13561 tctactcaac gcaattgggg cacttgataa tgtgacaatc ctccttgctc ctttccttgt 13621 cgcctcaaac aacgtcggga actgcacaga aacatcgcta cttattcacg cctcacctct 13681 ctttcctcct ttactcactt attcgcgcct cacctctctt tcctccttta ctcaagtcct 13741 ctgcttgccg ggtttattct tccgtttgtg ttgttgcggt ctgataggat ttgtttggag 13801 acgaaccttt ttttttgatt gctttataaa ttaacgtttt gccgaaattg tgtcgtaatc 13861 aagtattgcc ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa 13921 cttgaaaaag tggcaccgag tcggtgcttt tttcgcttta cgaattccca tggggagctt 13981 catcaagaga gcaaccaaca aagaagaggc gaacacggtg cacagcaccg tctgcatact 14041 tccgatttgc aattcacaac atatgataca tcatccatct ctctgagaaa actgggcact 14101 gttagaatct gggatttatc aacagccacc aactatctac tcaacgcaat tggggcactt 14161 gataatgtga caatcctcct tgctcctttc cttgtcgcct caaacaacgt cgggaactgc 14221 acagaaacat cgctacttat tcacgcctca cctctctttc ctcctttact cacttattcg 14281 cgcctcacct ctctttcctc ctttactcaa gtcctctgct tgccgggttt attcttccgt 14341 ttgtgttgtt gcggtctgat aggatttgtt tggagacgaa cctttttttt tgattgcttt 14401 ataaattaac gttttgccga aattgtgttt ccgatctaat gtccatgttt tagagctaga 14461 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt 14521 gcttttttcg ctcagagagg atgcacatgt gaccga //