LOCUS       Exported                6544 bp ds-DNA     circular SYN 17-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pICH47732 TP Fcp NAT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     36..52
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    56..1035
                     /note="FCP promoter"
     CDS             1036..1605
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /note="nat (domesticated)"
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     misc_feature    1606..2116
                     /note="FCP terminator"
     primer_bind     complement(2123..2139)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2180..2198)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(3911..4499)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4670..5530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5531..5635)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      5722..6353
                     /note="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gagctcgagg tcgacggtat cgataagctt
       61 gcgctttttc cgagaactcc ccataagtca acggctccaa tcaagaatgt atccgacaac
      121 ggcgagcata gcaacacgtc cgtctttgga gtagaatcat catgttgtgg atgaatacac
      181 agatgaatga cattaaaagc atgaacatgt tagagagtag gaggtagaga ttgatatggt
      241 agcattgcga tgtttgtttt tggtcagcat atgatgagtg gataccaata tgatgaaagt
      301 tgaatctcgc gtttgagctc agcggtacgt tattgatcga aagtagcctg atcaaaatcc
      361 ttggagagta caagaggatc aaagaatcca gtgggggcga taactccaag ctcgttctca
      421 aagaggcaat ggaggtagaa actcatccca gttgagaaga agtgaaggca gtggcggtgg
      481 cgaaagcaga ggcaacgagg acagacttcc tgtgggttga tgcaacgaat atttccagaa
      541 ggagaagttt agagagttga accgctacct acaatgacaa agtatcgtat cgattttgat
      601 gttggttggt tatgaattca aactgtaagt tggattgtga gaagatcaga agttgaacga
      661 acacatcttt ccgatcattc acctccacac tgcaacaaca cggtacttct tccgcggcag
      721 atctctgtcg ccattctctt gtcctgttgt tggctgtgag acgaggaaag caacgacaag
      781 tttcacaaaa gggagttcct ttaacgagat atgtttttta taaagagtcc caatagaaag
      841 acaaattgat tcctccgtgc aaacgcgcaa ataaacacca cgtccattat atccatatct
      901 ttcagagtat ccaacaagtg ttgaaggaca ggtagttgaa gtaacgtatc ttccccctcg
      961 actggatcca tcaacaaggc gaacaaatcc attcaacctc tcataaatta tctgatttac
     1021 caaaccgata ccaaaatgac cactcttgac gacacggctt accggtaccg caccagtgtc
     1081 ccgggggacg ccgaggccat cgaggcactg gatgggtcct tcaccaccga caccgtattc
     1141 cgcgtcaccg ccaccgggga cggcttcacc ctgcgggagg tgccggtgga cccgcccctg
     1201 accaaggtgt tccccgacga cgaatcggac gacgaatcgg acgccgggga ggacggcgac
     1261 ccggactccc ggacgttcgt cgcgtacggg gacgacggcg acctggcggg cttcgtggtc
     1321 gtctcgtact ccggctggaa ccgccggctg accgtcgagg acatcgaggt cgccccggag
     1381 caccgggggc acggggtcgg gcgcgcgttg atggggctcg cgacggagtt cgcccgcgag
     1441 cggggcgccg ggcacctctg gctggaggtc accaacgtca acgcaccggc gatccacgcg
     1501 taccggcgga tggggttcac cctctgcggc ctggacaccg ccctgtacga cggcaccgcc
     1561 tcggacggcg agcaggcgct ctacatgagc atgccctgcc cctgagcgcg gccgcatact
     1621 ggattggtga atcaatgagc cgtagcacaa tggttacatt cggctagcta agatccaatg
     1681 gcaaggacca agtgctggaa cttgttttgc tttagcagat cttagcgtga gaggtatttg
     1741 tcctctgtca ggagtagata gtagatgttc tttttaaact aaaatgctaa ctgttccgaa
     1801 ttcctcatcg cagctaatcc gtacatcaaa agacaaaatg ctaggtatgt gtactacatc
     1861 tcctgttgct agataagaca tatgatagga aacacaccat caatagtcat tgtagcttta
     1921 cttatactac gcatttgcac tttcccctga gtggcagagg cgcattgaga aaatcgatct
     1981 caacatagtt tatgtagcat cccctagatc cattacttta agtctccttc gtctttggtg
     2041 taggcatgtt ggacacaacg aggtaaaaca caacacaaac aatgtgtcca gcaaagtagt
     2101 agctgctcca gttctcccgg gggatccact agttctagag cggccggccg ccaccgcggt
     2161 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgctgcaat tgtcttctgc
     2221 acgaagtggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     2281 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
     2341 ccatttgtat gtgcatgcca accacagggt tccccagatc aggcgctggc tgctgaaccc
     2401 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
     2461 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
     2521 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
     2581 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
     2641 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
     2701 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
     2761 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
     2821 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
     2881 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
     2941 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
     3001 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
     3061 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
     3121 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
     3181 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
     3241 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
     3301 atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga acgctccacg
     3361 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
     3421 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
     3481 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
     3541 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
     3601 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
     3661 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
     3721 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
     3781 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
     3841 ttgtcgccgg catcgttgcg ccacatctag gatctgccag gaaccgtaaa aaggccgcgt
     3901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4621 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4801 tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcaaggt tattgtctca
     5581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacga
     5641 attggccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
     5701 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
     5761 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
     5821 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
     5881 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
     5941 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
     6001 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
     6061 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
     6121 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
     6181 aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac
     6241 aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag
     6301 ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtcgcaaagg
     6361 agatcctgat ctgactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     6421 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     6481 tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat gcagtgggcc
     6541 ccac
//
