LOCUS       Exported                7029 bp ds-DNA     circular SYN 08-DEC-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pICH47732_FcFcpShBle
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Amanda Hopes
  TITLE     Direct Submission
  JOURNAL   Exported 08 Dec 2015 from SnapGene Viewer 2.8.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    6..11
                     /note="BpiI"
                     /note="color: #a6acb3"
     misc_feature    14..17
                     /note="TGCC"
                     /note="color: #a6acb3"
     primer_bind     86..102
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        122..1107
                     /note="FCP Promoter"
                     /note="color: #c0c0c0"
     CDS             1108..1482
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="ShBle"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #993366"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      1483..2600
                     /note="FCP Terminator"
                     /note="color: #c0c0c0"
     primer_bind     complement(2627..2643)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    2691..2694
                     /note="GCAA"
                     /note="color: #a6acb3"
     misc_feature    2697..2702
                     /note="BpiI"
                     /note="color: #a6acb3"
     misc_feature    complement(2718..2845)
                     /note="RB short"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(2869..4350)
                     /note="RK2"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      complement(4396..4984)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5155..6015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="CarbR/AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5155 .. 5946 / #993366
                        2: 5947 .. 6015 / #993366 / signal sequence
                     Cleavage site after base 5946"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6016..6120)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      6207..6838
                     /note="oriV"
                     /note="incP origin of replication"
                     /note="color: #ffff00"
     misc_feature    6860..7007
                     /note="LB"
                     /note="color: #a6acb3"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gaggctgcaa ggcgattaag ttgggtaacg
       61 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtacc
      121 ccaaagtaag gcatagaaat aatctgcatg aaaagatcta acgatttcta tcagttcccc
      181 tacaaatgga tgcttagggg tgcttgtatg agtgtacgaa agagccaacc aaaaaataat
      241 tttttattct gatttttaat tttttgtgct aaaacatgct gcgctgtgta cttcctccgt
      301 gcaccggtaa acgcgcacgg aggaaataca gtgttttaga aaaacaacgc gcaaaaatgc
      361 caatctttat tattttgaag tgaagcgcgc tatttactgt tatcgcgcta atactgtaaa
      421 aattcgcgct gatcttgatg ataccacctt aactttgttt ctattttttg ataacaaata
      481 caaaatctac tagtattgtg caccctccta ctactagcta ctactaggga gtgtcgtact
      541 agtagtctaa aaatacgcgt gttcaacatg cgcgtgctag aagtatgatg cgtgtgtaac
      601 aaatttttaa aagattaaaa aaatctttat tttgagggat tggatgcgtg atagaatgga
      661 gaattgtgtt cgtgggattc attttgacct accaattaaa acgaataaaa atgtcagcgt
      721 cgattataga cacgaagtac agaccagaca gcgcgtcgac gtcagatttt tatggccaat
      781 cagattgttt ccaacacaca aatggaaaga aagaatgcat tctgtggaag agtgtacatc
      841 cgggggaatt tagaacgaaa accacgtcgg tgaattgtac acgcgacaat cctccaatcg
      901 tggataatcc aggatttttt gttgatgttg aactgcatat ttgccgatcc ttcatgcgaa
      961 ctccaaattt ctatatcatt ttttcagcaa aatccaatat ttttcagcaa aatccaaaaa
     1021 acaagccatc gttttaccgc tttcgatctt ctcataactt tattctttca acagtataaa
     1081 ctaccaaaaa caaacttata tatcaaaatg gccaagttga ccagtgccgt tccggtgctc
     1141 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     1201 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     1261 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     1321 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     1381 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     1441 gccggcaact gcgtgcactt cgtggccgag gagcaggact gagcatttta ttaatcctta
     1501 tttgatcgat tcattgattc gaatatagtg agacccttcc gttgacaact acaatcgatt
     1561 cctaatttaa ttatttagta gagttccttg agtttttgtc acaaatatat tatctacttc
     1621 tatcatatac tactattcat agctagctac gcattgtttc ctcaatccat acgcagttgt
     1681 actgttacat tcacaacccg tatccctcat gacctcttga ctgtatccta cagtacgtat
     1741 ttcctttatt tttctcttcc acgagctaca gtagccacac ccctacggta cggtacagta
     1801 ttgataaatc aaccgacgac agcgcctctc aactgccaaa cagctggaat gcgttcgttt
     1861 gagaatgacc ccaagacaaa agatccattt tcaacctaca gtaacgtccg cccacgtttg
     1921 caggatccaa aaatgcgagg aatcaatcca gtcgaactag tcgagaaagg gcaacaaaga
     1981 tccaatttcc gtactagaca gatgtacagt atggaatcga gtgaatcaaa ccagtcgatt
     2041 cagtcgaact agtcgaggat tgtaatcaat actgtacata gtttgcgctt tgccttccac
     2101 taagttaccg gtacaggagt agtagatttt gtgagatttt tcatgacaaa caaatcgaaa
     2161 agtaaaaaaa aaacctatta gaaatcaaca atgacgacga tgatgatgta acatgatgag
     2221 gaggataatc ataatagtga caattcaact atcgtatcac cttatttctt cacaatcagc
     2281 caaaagaaaa ttccctagag gtcagcagaa aaaattacga aaagaacggc gtactgtaca
     2341 gcaacggcga aggattgaca aatgaatagt attattagaa taaaaagtgt ttggttttcg
     2401 ttatcgttat atttatcggt attgttacta ttactattta gtttggttct attttttaat
     2461 agtaataata ataataataa tgatacaaca atcatagtag tgggcgttaa tggatttctt
     2521 ctaccaacaa gaacaacaat aacgccgaca tcagcacagc gactactgtt gtcgtctact
     2581 acagcacaac aacaacgact aggcatgcaa gcttggcgta atcatggtca tagctgtttc
     2641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgct gcaattgtct
     2701 tctgcacgaa gtggtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     2761 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     2821 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc agatcaggcg ctggctgctg
     2881 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     2941 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     3001 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     3061 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     3121 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     3181 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     3241 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     3301 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     3361 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     3421 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     3481 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     3541 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     3601 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     3661 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
     3721 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     3781 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcaagacga cgcgaacgct
     3841 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
     3901 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
     3961 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
     4021 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
     4081 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
     4141 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
     4201 tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat
     4261 acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct
     4321 cctgcttgtc gccggcatcg ttgcgccaca tctaggatct gccaggaacc gtaaaaaggc
     4381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4801 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5281 tgcaatgata ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc aaggttattg
     6061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6121 cacgaattgg ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc
     6181 ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc
     6241 ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat
     6301 aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa
     6361 acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc
     6421 ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt
     6481 agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat
     6541 ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca
     6601 cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc
     6661 ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta
     6721 tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     6781 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgc
     6841 aaaggagatc ctgatctgac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     6901 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     6961 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg tggatgcagt
     7021 gggccccac
//
