LOCUS pGL4.23_A82V_201 5198 bp ds-DNA circular SYN 29-NOV-2016 DEFINITION From Aaron Lin/Pardis Sabeti. ACCESSION . (modified) VERSION . KEYWORDS pGL4.23-CMV-GPfix. SOURCE null ORGANISM . REFERENCE 1 (bases 1 to 4655) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 30, 2015 from SnapGene Viewer 2.7.1 http://www.snapgene.com FEATURES Location/Qualifiers enhancer 30..409 misc_feature 410..593 misc_feature 593 /note="Missing 20 bp 3' end of promoter" /note="AGGTCTATATAAGCAGAGCT" promoter 618..649 /note="minP" /note="minimal TATA-box promoter with low basal activity" misc_feature 682..2712 /note="GP_dMLD-2005" /note="Geneious type: ORF" misc_feature 925..927 /note="Geneious type: SNP" misc_feature 926 /note="Geneious type: Editing History Replacement" misc_feature 1606..2148 /note="Geneious type: domain" polyA_signal 2752..2873 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(3292..3880) CDS complement(4080..4940) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" polyA_signal 5045..5093 /note="synthetic polyadenylation signal" misc_feature 5107..5198 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggcctaactg gccgccagat atacgcgttg acattgatta ttgactagtt attaatagta 61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt ggggcctcgg 601 cggccaagct tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc 661 ggtactgttg gtaaagccac catgggtgtt acaggaatat tgcagttacc tcgtgatcga 721 ttcaagagga catcattctt tctttgggta attatccttt tccaaagaac attttccatc 781 ccgcttggag ttatccacaa tagtacatta caggttagtg atgtcgacaa actagtttgt 841 cgtgacaaac tgtcatccac aaatcaattg agatcagttg gactgaatct cgaggggaat 901 ggagtggcaa ctgacgtgcc atctgtgact aaaagatggg gcttcaggtc cggtgtccca 961 ccaaaggtgg tcaattatga agctggtgaa tgggctgaaa actgctacaa tcttgaaatc 1021 aaaaaacctg acgggagtga gtgtctacca gcagcgccag acgggattcg gggcttcccc 1081 cggtgccggt atgtgcacaa agtatcagga acgggaccat gtgccggaga ctttgccttc 1141 cacaaagagg gtgctttctt cctgtatgat cgacttgctt ccacagttat ctaccgagga 1201 acgactttcg ctgaaggtgt cgttgcattt ctgatactgc cccaagctaa gaaggacttc 1261 ttcagctcac accccttgag agagccggtc aatgcaacgg aggacccgtc gagtggctat 1321 tattctacca caattagata tcaggctacc ggttttggaa ctaatgagac agagtacttg 1381 ttcgaggttg acaatttgac ctacgtccaa cttgaatcaa gattcacacc acagtttctg 1441 ctccagctga atgagacaat atatgcaagt gggaagagga gcaacaccac gggaaaacta 1501 atttggaagg tcaaccccga aattgataca acaatcgggg agtgggcctt ctgggaaact 1561 aaaaaaaacc tcactagaaa aattcgcagt gaagagttgt ctttcacagc tgtatcaaac 1621 ggacccaaaa acatcagtgg tcagagtccg gcgcgaactt cttccgaccc agagaccaac 1681 acaacaaatg aagaccacaa aatcatggct tcagaaaatt cctctgcaat ggttcaagtg 1741 cacagtcaag gaaggaaagc tgcagtgtcg catctgacaa cccttgccac aatctccacg 1801 agtcctcaac ctcccacaac caaaacaggt ccggacaaca gcacccataa tacacccgtg 1861 tataaacttg acatctctga ggcaactcaa gttggacaac atcaccgtag agcagacaac 1921 gacagcacag cctccgacac tccccccgcc acgaccgcag ccggaccctt aaaagcagag 1981 aacaccaaca cgagtaagag cgctgactcc ctggacctcg ccaccacgac aagcccccaa 2041 aactacagcg agactgctgg caacaacaac actcatcacc aagataccgg agaagagagt 2101 gccagcagcg ggaagctagg cttaattacc aatactattg ctggagtagc aggactgatc 2161 acaggcggga gaaggactcg aagagaagta attgtcaatg ctcaacccaa atgcaacccc 2221 aatttacatt actggactac tcaggatgaa ggtgctgcaa tcggattggc ctggatacca 2281 tatttcgggc cagcagccga aggaatttac acagaggggc taatgcacaa ccaagatggt 2341 ttaatctgtg ggttgaggca gctggccaac gaaacgactc aagctctcca actgttcctg 2401 agagccacaa ctgagctgcg aaccttttca atcctcaacc gtaaggcaat tgacttcctg 2461 ctgcagcgat ggggtggcac atgccacatt ttgggaccgg actgctgtat cgaaccacat 2521 gattggacca agaacataac agacaaaatt gatcagatta ttcatgattt tgttgataaa 2581 acccttccgg accaggggga caatgacaat tggtggacag gatggagaca atggataccg 2641 gcaggtattg gagttacagg tgttataatt gcagttatcg ctttattctg tatatgcaaa 2701 tttgtctttt agtctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca 2761 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa 2821 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca 2881 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca 2941 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga 3001 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 3061 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc 3121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 3241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 3481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3721 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 3841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 3901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 3961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4021 aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt 4081 taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata 4141 gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc 4201 agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac 4261 cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag 4321 tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga 4381 gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc 4441 aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca 4501 gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc 4561 atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc 4621 gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc 4681 tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc 4741 atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc 4801 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc 4861 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca 4921 cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt 4981 tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg 5041 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat 5101 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc 5161 tgtccccagt gcaagtgcag gtgccagaac atttctct //