LOCUS pGL4.23_WT_2014_ 4655 bp ds-DNA circular SYN 29-NOV-2016 DEFINITION From Aaron Lin/Pardis Sabeti. ACCESSION . (modified) VERSION . KEYWORDS pGL4.23-CMV-GPfix. SOURCE null ORGANISM . REFERENCE 1 (bases 1 to 4655) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 30, 2015 from SnapGene Viewer 2.7.1 http://www.snapgene.com FEATURES Location/Qualifiers enhancer 30..409 misc_feature 410..593 misc_feature 593 /note="Missing 20 bp 3' end of promoter" /note="AGGTCTATATAAGCAGAGCT" promoter 618..649 /note="minP" /note="minimal TATA-box promoter with low basal activity" misc_feature 682..2169 /note="GP_dMLD-2005" /note="Geneious type: ORF" polyA_signal 2209..2330 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(2749..3337) CDS complement(3537..4397) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" polyA_signal 4502..4550 /note="synthetic polyadenylation signal" misc_feature 4564..4655 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggcctaactg gccgccagat atacgcgttg acattgatta ttgactagtt attaatagta 61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt ggggcctcgg 601 cggccaagct tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc 661 ggtactgttg gtaaagccac catgggtgtt acaggaatat tgcagttacc tcgtgatcga 721 ttcaagagga catcattctt tctttgggta attatccttt tccaaagaac attttccatc 781 ccgcttggag ttatccacaa tagtacatta caggttagtg atgtcgacaa actagtttgt 841 cgtgacaaac tgtcatccac aaatcaattg agatcagttg gactgaatct cgaggggaat 901 ggagtggcaa ctgacgtgcc atctgcgact aaaagatggg gcttcaggtc cggtgtccca 961 ccaaaggtgg tcaattatga agctggtgaa tgggctgaaa actgctacaa tcttgaaatc 1021 aaaaaacctg acgggagtga gtgtctacca gcagcgccag acgggattcg gggcttcccc 1081 cggtgccggt atgtgcacaa agtatcagga acgggaccat gtgccggaga ctttgccttc 1141 cacaaagagg gtgctttctt cctgtatgat cgacttgctt ccacagttat ctaccgagga 1201 acgactttcg ctgaaggtgt cgttgcattt ctgatactgc cccaagctaa gaaggacttc 1261 ttcagctcac accccttgag agagccggtc aatgcaacgg aggacccgtc gagtggctat 1321 tattctacca caattagata tcaggctacc ggttttggaa ctaatgagac agagtacttg 1381 ttcgaggttg acaatttgac ctacgtccaa cttgaatcaa gattcacacc acagtttctg 1441 ctccagctga atgagacaat atatgcaagt gggaagagga gcaacaccac gggaaaacta 1501 atttggaagg tcaaccccga aattgataca acaatcgggg agtgggcctt ctgggaaact 1561 aaaaaaaacc tcactagaaa aattcgcagt gaagagttgt ctttcgcagg actgatcaca 1621 ggcgggagaa ggactcgaag agaagtaatt gtcaatgctc aacccaaatg caaccccaat 1681 ttacattact ggactactca ggatgaaggt gctgcaatcg gattggcctg gataccatat 1741 ttcgggccag cagccgaagg aatttacaca gaggggctaa tgcacaacca agatggttta 1801 atctgtgggt tgaggcagct ggccaacgaa acgactcaag ctctccaact gttcctgaga 1861 gccacaactg agctgcgaac cttttcaatc ctcaaccgta aggcaattga cttcctgctg 1921 cagcgatggg gtggcacatg ccacattttg ggaccggact gctgtatcga accacatgat 1981 tggaccaaga acataacaga caaaattgat cagattattc atgattttgt tgataaaacc 2041 cttccggacc agggggacaa tgacaattgg tggacaggat ggagacaatg gataccggca 2101 ggtattggag ttacaggtgt tataattgca gttatcgctt tattctgtat atgcaaattt 2161 gtcttttagt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg 2221 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 2281 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 2341 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt 2401 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc 2461 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 2521 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc 2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 3181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 3481 tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac 3541 cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg 3601 gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc 3661 gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag 3721 ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct 3781 atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt 3841 gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac 3901 tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc 3961 agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg 4021 gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg 4081 accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct 4141 tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc 4201 atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt 4261 tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt 4321 tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga 4381 aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac 4441 tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg 4501 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt 4561 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt 4621 ccccagtgca agtgcaggtg ccagaacatt tctct //