LOCUS       pGL4.23_A82V/T23        4655 bp ds-DNA     circular SYN 29-NOV-2016
DEFINITION  From Aaron Lin/Pardis Sabeti.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    pGL4.23-CMV-GPfix.
SOURCE      null
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2015 from SnapGene Viewer 2.7.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     enhancer        30..409
     misc_feature    410..593
     misc_feature    593
                     /note="Missing 20 bp 3' end of promoter"
                     /note="AGGTCTATATAAGCAGAGCT"
     promoter        618..649
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    682..2169
                     /note="GP_dMLD-2005"
                     /note="Geneious type: ORF"
     misc_feature    925..927
                     /note="Geneious type: SNP"
     misc_feature    926
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1369
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1369..1371
                     /note="Geneious type: SNP"
     misc_feature    2047..2049
                     /note="Geneious type: SNP"
     misc_feature    2048
                     /note="Geneious type: Editing History Replacement"
     polyA_signal    2209..2330
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2749..3337)
     CDS             complement(3537..4397)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     polyA_signal    4502..4550
                     /note="synthetic polyadenylation signal"
     misc_feature    4564..4655
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                      the human alpha-2 globin gene"
ORIGIN      
        1 ggcctaactg gccgccagat atacgcgttg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt ggggcctcgg
      601 cggccaagct tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc
      661 ggtactgttg gtaaagccac catgggtgtt acaggaatat tgcagttacc tcgtgatcga
      721 ttcaagagga catcattctt tctttgggta attatccttt tccaaagaac attttccatc
      781 ccgcttggag ttatccacaa tagtacatta caggttagtg atgtcgacaa actagtttgt
      841 cgtgacaaac tgtcatccac aaatcaattg agatcagttg gactgaatct cgaggggaat
      901 ggagtggcaa ctgacgtgcc atctgtgact aaaagatggg gcttcaggtc cggtgtccca
      961 ccaaaggtgg tcaattatga agctggtgaa tgggctgaaa actgctacaa tcttgaaatc
     1021 aaaaaacctg acgggagtga gtgtctacca gcagcgccag acgggattcg gggcttcccc
     1081 cggtgccggt atgtgcacaa agtatcagga acgggaccat gtgccggaga ctttgccttc
     1141 cacaaagagg gtgctttctt cctgtatgat cgacttgctt ccacagttat ctaccgagga
     1201 acgactttcg ctgaaggtgt cgttgcattt ctgatactgc cccaagctaa gaaggacttc
     1261 ttcagctcac accccttgag agagccggtc aatgcaacgg aggacccgtc gagtggctat
     1321 tattctacca caattagata tcaggctacc ggttttggaa ctaatgaggc agagtacttg
     1381 ttcgaggttg acaatttgac ctacgtccaa cttgaatcaa gattcacacc acagtttctg
     1441 ctccagctga atgagacaat atatgcaagt gggaagagga gcaacaccac gggaaaacta
     1501 atttggaagg tcaaccccga aattgataca acaatcgggg agtgggcctt ctgggaaact
     1561 aaaaaaaacc tcactagaaa aattcgcagt gaagagttgt ctttcgcagg actgatcaca
     1621 ggcgggagaa ggactcgaag agaagtaatt gtcaatgctc aacccaaatg caaccccaat
     1681 ttacattact ggactactca ggatgaaggt gctgcaatcg gattggcctg gataccatat
     1741 ttcgggccag cagccgaagg aatttacaca gaggggctaa tgcacaacca agatggttta
     1801 atctgtgggt tgaggcagct ggccaacgaa acgactcaag ctctccaact gttcctgaga
     1861 gccacaactg agctgcgaac cttttcaatc ctcaaccgta aggcaattga cttcctgctg
     1921 cagcgatggg gtggcacatg ccacattttg ggaccggact gctgtatcga accacatgat
     1981 tggaccaaga acataacaga caaaattgat cagattattc atgattttgt tgataaaacc
     2041 cttccgggcc agggggacaa tgacaattgg tggacaggat ggagacaatg gataccggca
     2101 ggtattggag ttacaggtgt tataattgca gttatcgctt tattctgtat atgcaaattt
     2161 gtcttttagt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     2221 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     2281 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     2341 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     2401 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc
     2461 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     2521 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac
     3541 cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg
     3601 gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc
     3661 gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag
     3721 ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct
     3781 atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt
     3841 gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac
     3901 tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc
     3961 agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg
     4021 gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg
     4081 accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct
     4141 tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc
     4201 atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt
     4261 tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt
     4321 tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga
     4381 aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac
     4441 tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg
     4501 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
     4561 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
     4621 ccccagtgca agtgcaggtg ccagaacatt tctct
//
