LOCUS       R702-X43-521            6770 bp    DNA     circular SYN 07-MAY-2014
DEFINITION  GST-RAF1-RBDkit
ACCESSION   R702-X43-521
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Entry Clone:   R702-E43, 3871 bps DNA circular (attL1, 
            attL2 sites)
            Destination Vector:   pDest-521, 7247 bps DNA circular (attR1, 
            attR2 sites)
            
            Recombination using LR clonase to produce Expression Clone
FEATURES             Location/Qualifiers
     CDS             1..17
                     /gene="T7"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_recomb     complement(96..117)
                     /gene="attB1"
                     /product="attB1 recombination site"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="attB1"
                     /SECDescr="attB1 recombination site"
     CDS             146..1300
                     /gene="GST-RAF1-RBDkit"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_recomb     1302..1323
                     /gene="attB2"
                     /product="attB2 recombination site"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="attB2"
                     /SECDescr="attB2 recombination site"
     CDS             1829..1875
                     /gene="ter"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(2484..3344)
                     /gene="bla"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             3507..4122
                     /gene="oriP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(4536..4727)
                     /gene="rop"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(5298..6380)
                     /gene="lacI"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agctagatag
       61 ctagaagctt gcggccgcac agctgtatac acatcacaag tttgtacaaa aaagttggct
      121 ttaactttaa gaaggagata tacatatgag tcctatcttg ggttactgga agattaaagg
      181 tttggtccaa ccaacgcgtc tgttgctgga gtacctggaa gaaaagtatg aagaacacct
      241 gtacgagcgt gacgaaggcg acaaatggcg caataaaaag ttcgagctgg gcctggagtt
      301 cccgaacctc ccgtattaca tcgacggtga tgttaaactg acgcagtcta tggctatcat
      361 tcgttacatt gcggacaagc ataacatgct gggtggctgc ccaaaagagc gcgctgagat
      421 ttccatgttg gaaggtgcgg tgctggatat tcgttatggt gttagccgta ttgcgtatag
      481 caaagatttt gaaacgctga aggttgactt tctgagcaag ctgcctgaga tgctgaagat
      541 gttcgaggat cgcctgtgcc acaagactta cctgaatggc gaccacgtga cccacccgga
      601 cttcatgctt tatgacgcac tggatgtcgt gctgtacatg gacccgatgt gtctggacgc
      661 ctttccgaaa ctggtctgct tcaagaagcg catcgaagcg atcccgcaga tcgataagta
      721 cctgaaaagc tctaagtata tcgcatggcc gctgcagggc tggcaagcca ccttcggtgg
      781 tggtgaccat ccgccgaaaa gcgacttggt tccgcgtggt tcgccgattc acattatgga
      841 acatatccag ggcgcatgga aaactatcag caatggcttc ggtttcaaag atgcggtgtt
      901 tgatggctcc agctgcatta gcccgaccat cgtacagcag ttcggttacc agcgtcgtgc
      961 gagcgatgat ggcaaactga ccgatccgag caagaccagc aacacgattc gcgtttttct
     1021 gccgaataaa caacgcaccg tcgtcaacgt tcgtaacggt atgagcctgc acgattgtct
     1081 gatgaaggct ctgaaagtgc gcggtctgca accggagtgt tgcgcagttt ttcgtctgtt
     1141 acacgagcac aagggtaaaa aggcccgtct ggactggaat accgacgcgg ccagcctgat
     1201 tggcgaggaa ctgcaagtcg attttctgga tcatgtgccg ctgacgaccc acaacttcgc
     1261 gcgtaaaacc tttttgaagc tgggtatcca tcgtgactaa tcaactttct tgtacaaagt
     1321 ggtgatgtgc aagccagcca gaactcgctc ctgaagaccc agaggatctc gagcaccacc
     1381 accaccacca ctaatgttaa ttaagttggg cgttcctagg ctgataaaac agaatttgcc
     1441 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     1501 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     1561 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1621 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     1681 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     1741 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
     1801 cattcaaata tgtatccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1861 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
     1921 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1981 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2041 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2101 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2161 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2221 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2281 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2341 ttttaacaaa atattaacgt ttacaatttc tggcggcacg atggcatgag attatcaaaa
     2401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3361 tcatgattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3421 ttagaaaaat aaacaaatag gtcatgacca aaatccctta acgtgagttt tcgttccact
     3481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3661 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4261 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     4321 tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc
     4381 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     4441 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4501 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4561 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     4621 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     4681 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     4741 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     4801 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     4861 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     4921 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     4981 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     5041 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     5101 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     5161 ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa ggagctgact
     5221 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     5281 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5341 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     5401 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     5461 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     5521 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     5581 gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     5641 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     5701 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     5761 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     5821 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     5881 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     5941 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     6001 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     6061 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     6121 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     6181 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     6241 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     6301 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     6361 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     6421 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     6481 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     6541 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     6601 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     6661 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     6721 ccacgatgcg tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa
//
