LOCUS       Exported                4560 bp ds-DNA     circular SYN 30-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLacIq-Wasabi
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Dan Piraner
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 30, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      109..156
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      193..648
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(763..1623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1624..1716)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      1797..2385
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2815..3006)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        3677..3754
                     /gene="lacI (mutant)"
                     /note="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3755..3757
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             3767..3784
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             3797..4507
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /note="mWasabi"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYKG"
ORIGIN
        1 ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct
       61 actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact agcataaccc
      121 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      181 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      241 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      301 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      361 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      421 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      481 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      541 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      601 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttct ggcggcacga
      661 tggcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1621 catactcttc ctttttcaat catgattgaa gcatttatca gggttattgt ctcatgagcg
     1681 gatacatatt tgaatgtatt tagaaaaata aacaaatagg tcatgaccaa aatcccttaa
     1741 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1801 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1861 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1921 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1981 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2041 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2161 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2341 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2401 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2461 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2521 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2581 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2641 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2701 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2761 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2821 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2881 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2941 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     3001 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     3061 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     3121 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     3181 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     3241 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3301 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3361 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3421 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc
     3481 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     3541 ctaatgagtg agctaactta cattaattgc gttgcgcacc attccttgcg gcggcggtgc
     3601 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     3661 gtcgagatcc cgcgttgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata
     3721 gcgcccggaa gagagtcaat tcagggtggt gaatatgggc agcagccatc accatcatca
     3781 ccacagccag gatccaatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc
     3841 cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga
     3901 gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga
     3961 gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcagtt acggcaacag
     4021 ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg
     4081 ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga
     4141 catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc
     4201 ccccaacggc cccgtgatgc agaaggagac caccggctgg gacgcctcca ccgagaggat
     4261 gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagatg aagctgctgc tggagggcgg
     4321 cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc
     4381 cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa
     4441 ggtgaccgtt tacgagatcg ccgtggcccg caactccacc gacggcatgg acgagctgta
     4501 caagggctga aagcttgcgg ccgcataatg cttaagtcga acagaaagta atcgtatgag
//
