LOCUS       Exported                4560 bp ds-DNA     circular SYN 30-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTlpAsp-Wasabi
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Dan Piraner
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 30, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        180..231
                     /bound_moiety="TlpA"
                     /note="TlpA Promoter (sp)"
     RBS             262..267
                     /note="ribosome binding site"
     CDS             275..277
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             287..304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             317..1027
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /note="mWasabi"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYKG"
     terminator      1189..1236
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1273..1728
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(1843..2703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2704..2796)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      2877..3465
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3895..4086)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
       61 ctaatgagtg agctaactta cattaattgc gttgcgcgca accgcacctg tggcgccggt
      121 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc ccgcgaaatt
      181 ttaatttgtt tgttagttag tttatttgtt ggtttgtttg tgtatttaaa tcctctagaa
      241 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc accatcatca
      301 ccacagccag gatccaatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc
      361 cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga
      421 gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga
      481 gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcagtt acggcaacag
      541 ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg
      601 ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga
      661 catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc
      721 ccccaacggc cccgtgatgc agaaggagac caccggctgg gacgcctcca ccgagaggat
      781 gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagatg aagctgctgc tggagggcgg
      841 cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc
      901 cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa
      961 ggtgaccgtt tacgagatcg ccgtggcccg caactccacc gacggcatgg acgagctgta
     1021 caagggctga aagcttgcgg ccgcataatg cttaagtcga acagaaagta atcgtatgag
     1081 ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct
     1141 actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact agcataaccc
     1201 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
     1261 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1321 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     1381 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     1441 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     1501 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     1561 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttct ggcggcacga
     1741 tggcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1801 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1861 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1921 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1981 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2041 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2101 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2161 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2221 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2281 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2341 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2401 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2461 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2521 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2581 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2641 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2701 catactcttc ctttttcaat catgattgaa gcatttatca gggttattgt ctcatgagcg
     2761 gatacatatt tgaatgtatt tagaaaaata aacaaatagg tcatgaccaa aatcccttaa
     2821 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2881 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2941 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3001 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3061 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3541 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3601 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     3661 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     3721 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     3781 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3841 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3901 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     3961 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     4021 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     4081 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     4141 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     4201 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     4261 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     4321 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     4381 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     4441 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     4501 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc
//
