LOCUS pCAP002_pGL3_zfh 5806 bp DNA linear 30-NOV-2016 FEATURES Location/Qualifiers rep_origin 1..456 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin PCR_primer 689..708 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region /current=0 polyA_signal 587..740 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal misc_feature 3191..3261 /note="MHC intron 16" misc_feature 3194..3260 /note="intron spiced out" polyA_signal 3491..3712 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal misc_marker complement(4815..5675) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) gene 1785..2444 /note="CamR" enhancer 1656..1670 /note="homology arm IF" misc_feature 2786..3091 /note="ccdB survival" enhancer 3133..3147 /note="homology arm IF" misc_feature 3272..3460 /note="truncated sgGFP" primer_bind 3794..3815 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site rep_origin 4053..4053 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin rep_origin 4041..4660 /note="pBR322 rep_ori" intron 753..1614 /note="Zfh1 enhancer" source 1..5806 /dnas_title="pCAP002_pGL3_zfh1_IF_CapSeq_cleaned" ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 721 aggtgccaga acatttctct atcgataggt acCTTAAAAG TGCCAGTGCT TGTGGCGTGC 781 ACGGAGAAAA AAACCTTGAG GTCATATTTG AAAAAAAAAA AAAAAAACTA AATTCCAAGT 841 GAGTAGTTTA CTCTTAGTCA CTATCGAGTG TGTGCTGTTT GTTTTTCGAA ATATGCATAT 901 CTTAGCTACT TGCGTTGCTG TTTTTTTTTT TTTTTTTTTT TTTTTGACGA TTTGTCAACA 961 GGAAATATGC ATAAGGTTGG CTCGGGATCC GGATCGGATG GCATGGGGTT TGATTGGATG 1021 CCGTACTTGG CACCGCACTG CCGTTATCGG TTGGCTCATT TCCCGTTAGC CGCGAACAAA 1081 GCCCACGCAT TGTGCTACGA CCGCCGTGAT GTGTTCAAAG ATCAAGTGCC CCCAGAACCA 1141 TCTCCTGCCA TCCCTGCCAT CCCTGCCCGC CTGCATCCAT CGCGGGCATT CAGATTCCGA 1201 CTGCGCATGA GCAGCGGTCT CCAACACGTA AGCAAGTGGA AGGCGAGGGA TAAGAACAAG 1261 TAACTTCTCT CTTATCGGAC GCTTCCAGGA ATTTCGATCC TCGCACCGCA TTCCGCAGGT 1321 TTAGTCACTG GATTCCACGG CTTTTCGAGC TGATTGTCCC AAGAAACATT ATTTTGGACA 1381 TGTGACTCAC TGACTCAAAG CTATTATCAC TTCTCGGACA TATGCGTGTA TATAAAATAT 1441 TTTTAAACAT TATTAGGTAT ACTATAAACT CTGCCTCATT CATGCCTACA GAACTTCAGC 1501 CTTGACATTC TATATATAGA AACCCTTACT ACGTCTTTAC TCTTTAAATA GTTCTTTGGA 1561 TATTATATTA GCACATTGAT GTGCTTTTTT TTTGGATGGT TGTGTCCAAC TCACggtacc 1621 gagctcttac gcgtgctagc ccgggcTCGA GATCTTAGAG CATGCACCGG TGATATCcgg 1681 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt 1741 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca 1801 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc 1861 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa 1921 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc 1981 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg 2041 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct 2101 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt 2161 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct 2221 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact 2281 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc 2341 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta 2401 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct 2461 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat 2521 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac 2581 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc 2641 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa 2701 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt 2761 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag 2821 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat 2881 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc 2941 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc 3001 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa 3061 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc 3121 tgcagGATAT CGTCGACGAA TTCGGCCCCT GCAGGGGCAT TCCGGTACTG TTGGTAAAGC 3181 CACCATGGAA AAGGTAGGTT CAACCACTGA TGCCTAGGCA CACCGAAACG ACTAACCCTA 3241 ATTCTTATCC TTTACTTCAG GCCACTTAAG catggctagc aaaggagaag aactcttcac 3301 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca agttctctgt 3361 cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt tcatctgcac 3421 tactggcaaa ctgcctgttc cctggccaac actagtctga tctagagtcg gggcGGCCGG 3481 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 3541 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 3601 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 3661 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat 3721 aaggatccga ccgatgccct CAAGCTTtga gagGGGCCcc cttcaaccca gtcagctccG 3781 ACGTCttggC gccCCcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 3841 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg 3901 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3961 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 4021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 4081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 4141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 4201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 4261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 4321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 4381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 4441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 4501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 4561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 4621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 4681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 4741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 4801 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 4861 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 4921 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 4981 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 5041 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 5101 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 5161 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 5221 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 5281 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 5341 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 5401 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 5461 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 5521 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 5581 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 5641 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 5701 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 5761 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg //