LOCUS       pCAP002_pGL3_zfh        5806 bp    DNA     linear       30-NOV-2016
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    3191..3261
                     /note="MHC intron 16"
     misc_feature    3194..3260
                     /note="intron spiced out"
     polyA_signal    3491..3712
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(4815..5675)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     gene            1785..2444
                     /note="CamR"
     enhancer        1656..1670
                     /note="homology arm IF"
     misc_feature    2786..3091
                     /note="ccdB survival"
     enhancer        3133..3147
                     /note="homology arm IF"
     misc_feature    3272..3460
                     /note="truncated sgGFP"
     primer_bind     3794..3815
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      4053..4053
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      4041..4660
                     /note="pBR322 rep_ori"
     intron          753..1614
                     /note="Zfh1 enhancer"
     source          1..5806
                     /dnas_title="pCAP002_pGL3_zfh1_IF_CapSeq_cleaned"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt acCTTAAAAG TGCCAGTGCT TGTGGCGTGC
      781 ACGGAGAAAA AAACCTTGAG GTCATATTTG AAAAAAAAAA AAAAAAACTA AATTCCAAGT
      841 GAGTAGTTTA CTCTTAGTCA CTATCGAGTG TGTGCTGTTT GTTTTTCGAA ATATGCATAT
      901 CTTAGCTACT TGCGTTGCTG TTTTTTTTTT TTTTTTTTTT TTTTTGACGA TTTGTCAACA
      961 GGAAATATGC ATAAGGTTGG CTCGGGATCC GGATCGGATG GCATGGGGTT TGATTGGATG
     1021 CCGTACTTGG CACCGCACTG CCGTTATCGG TTGGCTCATT TCCCGTTAGC CGCGAACAAA
     1081 GCCCACGCAT TGTGCTACGA CCGCCGTGAT GTGTTCAAAG ATCAAGTGCC CCCAGAACCA
     1141 TCTCCTGCCA TCCCTGCCAT CCCTGCCCGC CTGCATCCAT CGCGGGCATT CAGATTCCGA
     1201 CTGCGCATGA GCAGCGGTCT CCAACACGTA AGCAAGTGGA AGGCGAGGGA TAAGAACAAG
     1261 TAACTTCTCT CTTATCGGAC GCTTCCAGGA ATTTCGATCC TCGCACCGCA TTCCGCAGGT
     1321 TTAGTCACTG GATTCCACGG CTTTTCGAGC TGATTGTCCC AAGAAACATT ATTTTGGACA
     1381 TGTGACTCAC TGACTCAAAG CTATTATCAC TTCTCGGACA TATGCGTGTA TATAAAATAT
     1441 TTTTAAACAT TATTAGGTAT ACTATAAACT CTGCCTCATT CATGCCTACA GAACTTCAGC
     1501 CTTGACATTC TATATATAGA AACCCTTACT ACGTCTTTAC TCTTTAAATA GTTCTTTGGA
     1561 TATTATATTA GCACATTGAT GTGCTTTTTT TTTGGATGGT TGTGTCCAAC TCACggtacc
     1621 gagctcttac gcgtgctagc ccgggcTCGA GATCTTAGAG CATGCACCGG TGATATCcgg
     1681 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     1741 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1801 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1861 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1921 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1981 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     2041 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     2101 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2161 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2221 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2281 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2341 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     2401 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct
     2461 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     2521 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     2581 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     2641 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     2701 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     2761 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     2821 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     2881 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     2941 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     3001 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     3061 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     3121 tgcagGATAT CGTCGACGAA TTCGGCCCCT GCAGGGGCAT TCCGGTACTG TTGGTAAAGC
     3181 CACCATGGAA AAGGTAGGTT CAACCACTGA TGCCTAGGCA CACCGAAACG ACTAACCCTA
     3241 ATTCTTATCC TTTACTTCAG GCCACTTAAG catggctagc aaaggagaag aactcttcac
     3301 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca agttctctgt
     3361 cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt tcatctgcac
     3421 tactggcaaa ctgcctgttc cctggccaac actagtctga tctagagtcg gggcGGCCGG
     3481 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     3541 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3601 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3661 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     3721 aaggatccga ccgatgccct CAAGCTTtga gagGGGCCcc cttcaaccca gtcagctccG
     3781 ACGTCttggC gccCCcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     3841 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     3901 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3961 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4801 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4861 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4921 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4981 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5041 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5101 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5161 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5221 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5281 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5341 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5401 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5461 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5521 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5581 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5641 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5701 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5761 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//

