LOCUS Exported 5765 bp ds-DNA circular SYN 02-JUN-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSTAP-seq_fly-tj SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5765) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 2, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5765 /organism="synthetic DNA construct" /note="dnas_title=pSTAP-seq_fly-tj" rep_origin 1..456 /label=f1 origin /note="dnas_title=f1 origin vntifkey=33" polyA_signal 587..740 /label=Synthetic (upstream) poly (A)signal /note="dnas_title=Synthetic (upstream) poly (A)signal vntifkey=25" misc_feature 689..708 /label=PCR_primer /label=RVprimer3 binding region /label=PCR_primer /note="current=0 dnas_title=RVprimer3 binding region vntifkey=28" enhancer 754..1608 /note="tj enhancer" enhancer 1615..1629 /note="homology arm IF" gene 1744..2403 /label=CamR misc_feature 2745..3050 /label=ccdB survival enhancer 3092..3106 /note="homology arm IF" misc_feature 3150..3220 /label=MHC intron 16 misc_feature 3153..3219 /label=intron spiced out misc_feature 3231..3419 /label=truncated sgGFP polyA_signal 3450..3671 /label=SV40 late poly (A) signal /note="dnas_title=SV40 late poly (A) signal vntifkey=25" primer_bind 3753..3774 /label=RVprimer4 binding site /note="dnas_title=RVprimer4 binding site vntifkey=28" rep_origin 4000..4619 /label=pBR322 rep_ori rep_origin 4012 /label=ColE1-derived plasmid replication origin /note="dnas_title=ColE1-derived plasmid replication origin vntifkey=33" misc_feature complement(4774..5634) /label=misc_marker /label=Beta-lactamase gene (Ampr) /label=misc_marker /note="dnas_title=Beta-lactamase gene (Ampr) vntifkey=22" ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 721 aggtgccaga acatttctct atcgataggt acctcagtgc aactgtaaac attgatacgg 781 attaaaagat tcattgaatg ctcatgaact gagagttacg ttcgtttctt tggatgcttg 841 atagtttaaa cattgaagat cgtaaaaata tagaatgctc agtttttcat agtattcaaa 901 ttgtattcta ttttgtcccc atgccacatt catattcata tctgggtttt agaaaccacc 961 gagtgggtct cagcttgcat catagatttt gggtttcatc tcgccgtttt ccgagaagtg 1021 gcatcttctg tgtggtctgt cggcatggat aaatgggctt ccagcgacga cgacccccgc 1081 gggcaacagt ttaagaatca aaataaacac ttcgatcccg atccctctgg acttcgataa 1141 gttatgtcaa caaacttcgg tcgcgtaacg gcgcatgcag gagctctgga gtagtaggag 1201 ctccagctga agtgcagcat tgggttacaa tcgtccacat ggaggagatc agcatcagcc 1261 cctaggaggg ggagggggcg gtgttggaac tttggagcag tccagcattc agttggggcg 1321 gcgtaattcg gcaataatca gagggccgta tctacatatc tgcattttca aaccgtaata 1381 aatccctaat aaaaatcgtt tcctttggcc tgtttatttt catttcaatt tcgattcact 1441 tttgcggtgt cgcctcgcta cgcttttttc agaatctgaa tttgttattt ttattttgat 1501 tatatgagtt tcctgcccta ccaagaaccc aaatctctta ggagcttatg taaccagacg 1561 aaaccactcg atcgaagagt tgcgcaagta tttttgatga aattcgggag atcttagagc 1621 atgcaccggt gatatccggc cgcattaggc accccaggct ttacacttta tgcttccggc 1681 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct 1741 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa 1801 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg 1861 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt 1921 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac 1981 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact 2041 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata 2101 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt 2161 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac 2221 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa 2281 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc 2341 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg 2401 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat 2461 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg 2521 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat 2581 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct 2641 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat 2701 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt 2761 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg 2821 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag 2881 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca 2941 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc 3001 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct 3061 cccttataca cagccagtct gcaggatatc gtcgacgaat tcggcccctg caggggcatt 3121 ccggtactgt tggtaaagcc accatggaaa aggtaggttc aaccactgat gcctaggcac 3181 accgaaacga ctaaccctaa ttcttatcct ttacttcagg ccacttaagc atggctagca 3241 aaggagaaga actcttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 3301 acggccacaa gttctctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 3361 ccctgaagtt catctgcact actggcaaac tgcctgttcc ctggccaaca ctagtctgat 3421 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 3481 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 3541 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 3601 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 3661 caaatgtggt aaaatcgata aggatccgac cgatgccctc aagctttgag aggggccccc 3721 ttcaacccag tcagctccga cgtcttggcg cccccgcggg gcatgactat cgtcgccgca 3781 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc 3841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4441 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4741 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 4801 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 4861 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 4921 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 4981 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5041 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 5101 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 5161 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 5221 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 5281 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5341 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5401 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5461 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5521 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5581 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 5641 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 5701 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 5761 acctg //