LOCUS       Exported                5765 bp ds-DNA     circular SYN 02-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSTAP-seq_fly-tj
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="synthetic DNA construct"
                     /note="dnas_title=pSTAP-seq_fly-tj"
     rep_origin      1..456
                     /label=f1 origin
                     /note="dnas_title=f1 origin
                     vntifkey=33"
     polyA_signal    587..740
                     /label=Synthetic (upstream) poly (A)signal
                     /note="dnas_title=Synthetic (upstream) poly (A)signal
                     vntifkey=25"
     misc_feature    689..708
                     /label=PCR_primer
                     /label=RVprimer3 binding region
                     /label=PCR_primer
                     /note="current=0
                     dnas_title=RVprimer3 binding region
                     vntifkey=28"
     enhancer        754..1608
                     /note="tj enhancer"
     enhancer        1615..1629
                     /note="homology arm IF"
     gene            1744..2403
                     /label=CamR
     misc_feature    2745..3050
                     /label=ccdB survival
     enhancer        3092..3106
                     /note="homology arm IF"
     misc_feature    3150..3220
                     /label=MHC intron 16
     misc_feature    3153..3219
                     /label=intron spiced out
     misc_feature    3231..3419
                     /label=truncated sgGFP
     polyA_signal    3450..3671
                     /label=SV40 late poly (A) signal
                     /note="dnas_title=SV40 late poly (A) signal
                     vntifkey=25"
     primer_bind     3753..3774
                     /label=RVprimer4 binding site
                     /note="dnas_title=RVprimer4 binding site
                     vntifkey=28"
     rep_origin      4000..4619
                     /label=pBR322 rep_ori
     rep_origin      4012
                     /label=ColE1-derived plasmid replication origin
                     /note="dnas_title=ColE1-derived plasmid replication origin
                     vntifkey=33"
     misc_feature    complement(4774..5634)
                     /label=misc_marker
                     /label=Beta-lactamase gene (Ampr)
                     /label=misc_marker
                     /note="dnas_title=Beta-lactamase gene (Ampr)
                     vntifkey=22"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt acctcagtgc aactgtaaac attgatacgg
      781 attaaaagat tcattgaatg ctcatgaact gagagttacg ttcgtttctt tggatgcttg
      841 atagtttaaa cattgaagat cgtaaaaata tagaatgctc agtttttcat agtattcaaa
      901 ttgtattcta ttttgtcccc atgccacatt catattcata tctgggtttt agaaaccacc
      961 gagtgggtct cagcttgcat catagatttt gggtttcatc tcgccgtttt ccgagaagtg
     1021 gcatcttctg tgtggtctgt cggcatggat aaatgggctt ccagcgacga cgacccccgc
     1081 gggcaacagt ttaagaatca aaataaacac ttcgatcccg atccctctgg acttcgataa
     1141 gttatgtcaa caaacttcgg tcgcgtaacg gcgcatgcag gagctctgga gtagtaggag
     1201 ctccagctga agtgcagcat tgggttacaa tcgtccacat ggaggagatc agcatcagcc
     1261 cctaggaggg ggagggggcg gtgttggaac tttggagcag tccagcattc agttggggcg
     1321 gcgtaattcg gcaataatca gagggccgta tctacatatc tgcattttca aaccgtaata
     1381 aatccctaat aaaaatcgtt tcctttggcc tgtttatttt catttcaatt tcgattcact
     1441 tttgcggtgt cgcctcgcta cgcttttttc agaatctgaa tttgttattt ttattttgat
     1501 tatatgagtt tcctgcccta ccaagaaccc aaatctctta ggagcttatg taaccagacg
     1561 aaaccactcg atcgaagagt tgcgcaagta tttttgatga aattcgggag atcttagagc
     1621 atgcaccggt gatatccggc cgcattaggc accccaggct ttacacttta tgcttccggc
     1681 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     1741 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1801 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1861 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1921 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     1981 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     2041 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     2101 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     2161 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     2221 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     2281 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     2341 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     2401 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     2461 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     2521 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     2581 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     2641 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     2701 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     2761 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     2821 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     2881 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     2941 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     3001 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     3061 cccttataca cagccagtct gcaggatatc gtcgacgaat tcggcccctg caggggcatt
     3121 ccggtactgt tggtaaagcc accatggaaa aggtaggttc aaccactgat gcctaggcac
     3181 accgaaacga ctaaccctaa ttcttatcct ttacttcagg ccacttaagc atggctagca
     3241 aaggagaaga actcttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     3301 acggccacaa gttctctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     3361 ccctgaagtt catctgcact actggcaaac tgcctgttcc ctggccaaca ctagtctgat
     3421 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     3481 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3541 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     3601 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     3661 caaatgtggt aaaatcgata aggatccgac cgatgccctc aagctttgag aggggccccc
     3721 ttcaacccag tcagctccga cgtcttggcg cccccgcggg gcatgactat cgtcgccgca
     3781 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     3841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4441 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4741 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4801 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4861 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4921 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4981 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5041 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5101 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5161 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5221 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5281 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5341 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5401 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5461 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5521 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5581 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5641 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5701 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5761 acctg
//
