LOCUS       pSTAP-seq_fly-zf        4582 bp    DNA     linear       30-NOV-2016
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    1967..2037
                     /note="MHC intron 16"
     misc_feature    1970..2036
                     /note="intron spiced out"
     polyA_signal    2267..2488
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(3591..4451)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     misc_feature    2048..2236
                     /note="truncated sgGFP"
     primer_bind     2570..2591
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      2829..2829
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      2817..3436
                     /note="pBR322 rep_ori"
     intron          753..1614
                     /note="Zfh1 enhancer"
     enhancer        1909..1923
                     /note="homology arm IF"
     PCR_primer      complement(1875..1908)
                     /note="illumina adaptor rv"
     PCR_primer      1671..1703
                     /note="illumina adapter fw"
     enhancer        1656..1670
                     /note="homology arm IF"
     misc_feature    1704..1874
                     /note="dpse ush-2 promoter"
     source          1..4582
                     /dnas_title="pSTAP-seq_fly-zfh1_spikeIn-4"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt acCTTAAAAG TGCCAGTGCT TGTGGCGTGC
      781 ACGGAGAAAA AAACCTTGAG GTCATATTTG AAAAAAAAAA AAAAAAACTA AATTCCAAGT
      841 GAGTAGTTTA CTCTTAGTCA CTATCGAGTG TGTGCTGTTT GTTTTTCGAA ATATGCATAT
      901 CTTAGCTACT TGCGTTGCTG TTTTTTTTTT TTTTTTTTTT TTTTTGACGA TTTGTCAACA
      961 GGAAATATGC ATAAGGTTGG CTCGGGATCC GGATCGGATG GCATGGGGTT TGATTGGATG
     1021 CCGTACTTGG CACCGCACTG CCGTTATCGG TTGGCTCATT TCCCGTTAGC CGCGAACAAA
     1081 GCCCACGCAT TGTGCTACGA CCGCCGTGAT GTGTTCAAAG ATCAAGTGCC CCCAGAACCA
     1141 TCTCCTGCCA TCCCTGCCAT CCCTGCCCGC CTGCATCCAT CGCGGGCATT CAGATTCCGA
     1201 CTGCGCATGA GCAGCGGTCT CCAACACGTA AGCAAGTGGA AGGCGAGGGA TAAGAACAAG
     1261 TAACTTCTCT CTTATCGGAC GCTTCCAGGA ATTTCGATCC TCGCACCGCA TTCCGCAGGT
     1321 TTAGTCACTG GATTCCACGG CTTTTCGAGC TGATTGTCCC AAGAAACATT ATTTTGGACA
     1381 TGTGACTCAC TGACTCAAAG CTATTATCAC TTCTCGGACA TATGCGTGTA TATAAAATAT
     1441 TTTTAAACAT TATTAGGTAT ACTATAAACT CTGCCTCATT CATGCCTACA GAACTTCAGC
     1501 CTTGACATTC TATATATAGA AACCCTTACT ACGTCTTTAC TCTTTAAATA GTTCTTTGGA
     1561 TATTATATTA GCACATTGAT GTGCTTTTTT TTTGGATGGT TGTGTCCAAC TCACggtacc
     1621 gagctcttac gcgtgctagc ccgggcTCGA GATCTTAGAG CATGCACCGG ACACTCTTTC
     1681 CCTACACGAC GCTCTTCCGA TCTCCGGCCG CCCCAGCAGC GCCTGCCACT GCCACACTGC
     1741 CACTGCCTGG TGCCGTGCCG TGCCCCGAGT TTCAGTTTGC CATAGAACGC GGACCGAGCA
     1801 GCAAGCGGAC ACTCGACACT CAACACTCGA CACTCGACAA GCGAGAGATA GAGATATAAT
     1861 CACGTTCGTC ATACAGATCG GAAGAGCACA CGTCTGAACT CCAGTCACTC GACGAATTCG
     1921 GCCCCTGCAG GGGCATTCCG GTACTGTTGG TAAAGCCACC ATGGAAAAGG TAGGTTCAAC
     1981 CACTGATGCC TAGGCACACC GAAACGACTA ACCCTAATTC TTATCCTTTA CTTCAGGCCA
     2041 CTTAAGcatg gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga
     2101 attagatggt gatgttaacg gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc
     2161 aacatacgga aaacttaccc tgaagttcat ctgcactact ggcaaactgc ctgttccctg
     2221 gccaacacta gtctgatcta gagtcggggc GGCCGGccgc ttcgagcaga catgataaga
     2281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2401 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2461 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgaccga tgccctCAAG
     2521 CTTtgagagG GGCCcccttc aacccagtca gctccGACGT CttggCgccC Ccgcggggca
     2581 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     2641 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3241 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4561 tttccccgaa aagtgccacc tg
//

