LOCUS pGL3\Promoter\sg 4080 bp DNA circular 12-NOV-2008 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|498754724| COMMENT VNTDBDATE|498754762| COMMENT LSOWNER| COMMENT VNTNAME|pGL3 Promoter sgGFP| COMMENT VNTAUTHORNAME|Jenuwein| FEATURES Location/Qualifiers primer_bind 3018..3037 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region polyA_signal 2916..3069 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal rep_origin 2330..2785 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin misc_marker complement(1338..2198) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) gene 3347..4999 /note="luc+ firefly" primer_bind 319..338 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site polyA_signal 30..251 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal misc_feature 3149..3303 /note="DSCP Drosophila synthetic core promoter" misc_feature 3206..3211 /note="Inr" misc_feature 3177..3183 /note="TATA" misc_feature 3235..3240 /note="DPE" rep_origin 576..576 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin rep_origin 564..1183 /note="pBR322 ori" source 1..5001 /dnas_title="pGL3 Promoter sgGFP" ORIGIN 1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag 301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa 2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac 2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca 3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa 3061 catttctcta tcgataggta ccgagctctt aCGCGTCCAT GCATGCTATG GGAATTCCCA 3121 TCAGACGTCT GGCTCGAGAT CTGCGATCga gctcgcccgg ggatcgagcg cagcggtata 3181 aaagggcgcg gggtggctga gagcatcagt tgtgaatgaa tgttcgagcc gagcagacgt 3241 gccgctgcct tcgttaatat cctttgaata agccaacttt gaatcacaag acgcatacca 3301 aacCCTGCAg gAagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa 3361 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca 3421 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc 3481 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga 3541 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc 3601 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc 3661 gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt 3721 ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat 3781 catccaaaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta 3841 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc 3901 cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc 3961 taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc 4021 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca 4081 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat 4141 gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc 4201 gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga 4261 tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga 4321 agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac 4381 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt 4441 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa 4501 tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc 4561 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta 4621 ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa 4681 aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt 4741 cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt 4801 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca 4861 agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg 4921 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg 4981 cggaaagatc gccgtgtaat t //