LOCUS Exported 7919 bp ds-DNA circular SYN 13-JUN-2015 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS 3xFlag_KDM5B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7919) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Tuesday, November 22, 2016 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7919 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 855..884 /note="lac" misc_binding 889..911 /note="LacO" primer_bind 917..937 /note="M13-rev" primer_bind 955..974 /note="T3" misc_feature 997..1040 /note="Xen b-globin 5'UTR" CDS 1069..1134 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /note="3xFLAG" /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1180..5814 /codon_start=1 /note="KDM5B" /translation="MEPATTLPPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADP FAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFL DQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAP GKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKPHDIPQRQSVQPA ETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCTTPKWENEKEMKSTIKQEP TEKKDCELESEKEKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLV PPLHDVPKGDWRCPKCLAQECNKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVH MVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSG WNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPK TWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAG EFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICK MASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIK CKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAE SYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCAS VAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVED FQQQSQKLLSEEMPSAAELQELLDVSFEFDVELPQLTEMRIRLEQARWLEEVQQACLDS SSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLRARPRHSLSS LATAVKEMEEIPAYLPNGTVLKDSVQRARDWVQDVDALQAGGRVPVLETLIELVARGRS IPVHLNSLPRLEMLVAEVHAWKECAAKTFLPENSTYSLLEVLCPRCDIGLLGLKRKQRK LKEPLPSGKKRSTKLESLSDLERALMESKETAAAMATLGEARLREMEALQSLRFANEEK LLSPVQDLEMKVCLCQKTPATPMIQCELCRDAFHTSCVAAPSISQSSRIWLCPHCRRSE KPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKLVQDQVGS GLLSSRWPASAGQASATDKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGQSCLPLHGLS PEVNELLMEAQLLQVSLPEIQELYQTLLTKPSSVQQADRSSPVRSSSEKNDCLRGKRDA INSPERKLKRRPEREGLPSERWDRVKHMRTPQKKKIKLSHPKDMDSFKLERERSYDLVR NAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGV SPEMAEKEDYICVRCTGKDAPSRK" misc_feature 5816..6024 /note="Xen b-globin 3'UTR" primer_bind complement(6061..6080) /note="T7" primer_bind complement(6087..6104) /note="M13-fwd" CDS 6175..6243 /note="LacZ alpha" rep_origin 6254..6694 /direction=RIGHT /note="F1 ori" promoter 6761..6789 /note="Amp prom" CDS 7029..7688 /note="AmpR" ORIGIN 1 gcagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 61 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 901 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac 961 cctcactaaa gggaacaaaa gctggagcta agcttgcttg ttctttttgc agaagctcag 1021 aataaacgct caactttggc agatctgaat tcctgcagcc cggggatgga ctacaaagac 1081 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagggatct 1141 gcaagaagag catctgtgga attcctgcag cccgggtcca tggagccggc caccacgctg 1201 cccccaggcc cgcgcccggc gctgcccctc gggggcccgg gcccgctggg agagttcctg 1261 cctccccccg agtgtccggt cttcgagccg agctgggaag agttcgcgga ccccttcgct 1321 ttcatccaca agatccggcc catagccgag cagactggga tctgtaaggt gcggccgccg 1381 ccggattggc agccaccatt cgcttgtgat gtcgataaac ttcatttcac cccgcggatc 1441 cagaggctga atgagctgga ggcccaaaca cgtgtaaaat tgaatttcct ggaccagatt 1501 gcaaagtatt gggagctaca aggaagtact ctgaagattc cccacgtgga gagaaagatc 1561 ttagacttgt tccagctaaa taagttagtc gcagaagaag gtgggtttgc agttgtttgc 1621 aaggatagaa aatggaccaa aattgccacc aagatgggtt ttgctcctgg taaagctgtg 1681 ggctcacata tcaggggaca ttatgagcga atactcaatc catacaattt gttcctgtct 1741 ggagacagtc tgaggtgtct tcagaagcct aacctgacct ccgacacaaa ggacaaggag 1801 tacaaacccc atgatattcc ccagaggcag tctgtgcagc ccgcagaaac ctgtcctccg 1861 gcccgccgag caaagcgcat gagagcagag gctatgaata ttaaaattga gccagaagag 1921 gcaacagaag ccaggactca taatctgaga cgccgaatgg gttgcacaac tccaaaatgg 1981 gaaaatgaga aagaaatgaa aagcaccatc aagcaagagc ccactgagaa gaaagactgc 2041 gagttagaaa gtgagaagga gaagcccaag agccgggcca agaaaacggc cactgcggtg 2101 gacctgtatg tgtgcctctt atgtggaagt ggcaatgacg aggaccgact cctactgtgt 2161 gacggctgtg atgacagtta ccacaccttc tgcctggttc cccctctcca tgacgttccc 2221 aagggagact ggaggtgtcc taagtgtttg gctcaagaat gtaataagcc acaagaagct 2281 tttggttttg aacaagcagc aagggactat acacttcgta catttggaga aatggcagat 2341 gcattcaaat ctgattactt caacatgcca gtccatatgg ttcccacaga gctggtggag 2401 aaggagtttt ggcggctcgt aagcactatt gaagaagacg tcacggtgga gtatggagct 2461 gatattgctt caaaagagtt tggcagtggc tttcctgttc gagatgggaa aatcaagatt 2521 tctcctgagg aagaggaata cctggatagt ggctggaatt tgaacaacat gccagtgatg 2581 gagcagtctg tccttgctca tattactgct gatatatgtg gcatgaaact cccctggttg 2641 tatgtgggca tgtgcttttc ttcattctgt tggcacattg aagaccactg gagctattca 2701 atcaactacc tacactgggg tgagccaaaa acctggtatg gagttccagg ctacgctgct 2761 gagcagctag aaaacgtgat gaagaagctc gctcctgagc tctttgtgtc ccagccggac 2821 ctgctccacc agctggtgac catcatgaac cccaacactc tgatgactca tgaagtgcct 2881 gtttatcgaa cgaatcagtg tgctggggag tttgtaatta cgtttccaag agcctaccac 2941 agtggcttca accaaggttt taattttgcc gaggctgtta acttctgcac tgttgattgg 3001 ctgccattgg gccgccagtg tgtggagcat tatcgcttgc tgcaccgtta ttgtgtcttt 3061 tcccacgatg aaatgatatg caaaatggct tctaaggctg atgtattaga cgttgtggta 3121 gcgtcaactg ttcagaagga catggccatc atgatcgagg acgagaaagc tttaagggaa 3181 actgttcgga aattgggagt aattgattct gaaagaatgg attttgagct gttgccagat 3241 gatgagcggc agtgtataaa atgcaaaact acatgcttca tgtctgccat ctcctgctct 3301 tgtaaacctg gcctacttgt ttgcctacat catgtgaaag aattgtgttc ttgccccccg 3361 tataagtata acctgcggta tagatacact ctggatgatc tctatccaat gatgaatgca 3421 ttaaagcttc gagcagagtc ttacaatgaa tgggccttaa atgtgaatga agccttggaa 3481 gcaaagatca ataagaagaa aagtcttgtc agctttaaag ctttgattga agaatcagaa 3541 atgaaaaaat tcccagacaa tgatcttttg cgtcaccttc gactagtcac gcaggatgca 3601 gaaaagtgtg cctctgttgc ccagcagttg cttaatggca aaaggcaaac aaggtatcga 3661 tctggtggag gaaagtctca gaatcagctg acggtgaatg aacttagaca gtttgtgact 3721 cagctgtatg ctctgccgtg cgtccttagt cagacaccgt tgctaaagga tctcttgaat 3781 cgtgtggaag atttccaaca gcaaagccaa aaactactgt ctgaggaaat gcctagcgct 3841 gccgagctac aggagttgct ggatgtcagc tttgaatttg atgttgagct tccacagctc 3901 actgagatgc gcattcgctt agagcaggcc cgctggctgg aagaggttca gcaggcttgc 3961 ctggactcca gctccctttc tttggatgat atgagacgac tcatagactt aggggtgggg 4021 ctggctccat attctgctgt ggagaaagct atggcccgcc tgcaggagct gctaacagtg 4081 tcagaacact gggacgacaa agccaagagt ctcctcagag caagaccacg gcattctttg 4141 agtagccttg ctacagcagt caaagagatg gaggagatcc ccgcctacct gcccaatggt 4201 acagtcctga aggattctgt gcaaagagcc agagactggg ttcaggatgt ggatgcattg 4261 caggcaggag gacgtgtgcc agtgttagag acactcattg aacttgttgc aagaggccga 4321 tctatcccag tacatctcaa ttctttacca agattggaaa tgttagtagc tgaagttcat 4381 gcatggaaag aatgtgctgc taaaacattc ttgcctgaga attctacata ttctctgtta 4441 gaggttcttt gccctcggtg tgacattggc cttttggggc tgaaaaggaa acagagaaag 4501 ttgaaggagc ccttgccgag tggaaagaag agaagcacca agttagagag cctgagcgat 4561 ctggagaggg ctctgatgga gagcaaagag actgcggcag ctatggcaac tcttggggaa 4621 gctcgcctac gagagatgga agctttgcag tctctcagat ttgccaatga agagaaattg 4681 ctgtcacctg tccaagactt agagatgaaa gtctgcttgt gtcagaagac tccagccact 4741 cccatgattc agtgtgaact ctgcagggat gctttccata ctagttgtgt ggcagcaccc 4801 agtatttcac aaagctcccg aatctggctt tgtcctcact gtcggaggtc agagaagcct 4861 ccattagaga aaattctgcc tctgctggcc tccctacagc gtatacgagt tcgtcttcct 4921 gaaggagatg cactccgata catgattgaa agaaccgtga actggcaaca tagagcccag 4981 cagctgcttt cttcagggaa tctaaaactt gtgcaagacc aagtgggttc tggactgttg 5041 tctagcagat ggccagcctc agcaggacag gcatcagcaa cagacaaggt gtctcagcct 5101 cctggcacaa catccttttc attgcccgat gactgggaca acagaacctc atatttacac 5161 tctcccttct ctactggaca gagttgtctc cccctccatg gtttgagtcc agaagtgaat 5221 gaattgctga tggaagccca gctgctccag gtatcccttc ctgaaattca ggagctttat 5281 cagactctac ttactaagcc aagctctgtt cagcaagctg accgaagttc accagttaga 5341 tccagcagtg agaagaacga ttgccttcga gggaaacgag atgcaatcaa cagtcctgag 5401 aggaaactga aaaggcgccc agaaagagaa ggccttccta gcgagcggtg ggatcgagtt 5461 aaacacatgc ggacccccca aaagaagaaa atcaaactga gtcaccccaa ggacatggac 5521 agtttcaagt tagaaagaga gcgtagctat gacttagtgc gtaatgctga aactcattcc 5581 ctgccctcag acacatccta ctccgaacag gaggactctg aggatgaaga tgccatctgt 5641 ccagctgtga gctgcctgca gccagaagga gatgaggtgg actgggtcca gtgtgatggc 5701 agctgcaatc agtggtttca ccaggtctgt gttggtgtct ctccagagat ggcagagaag 5761 gaagactaca tctgtgtgcg ctgtactggg aaggacgcac caagccgaaa gtaaagcggc 5821 cgcggatctg gttaccacta aaccagcctc aagaacaccc gaatggagtc tctaagctac 5881 ataataccaa cttacacttt acaaaatgtt gtcccccaaa atgtagccat tcgtatctgc 5941 tcctaataaa aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa aaaacccccc 6001 cccccccccc cccccccccc cccctgcagg gccaagtcgg ccggaaccag ctccaattcg 6061 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa 6121 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 6181 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 6241 tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 6301 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 6361 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 6421 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 6481 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 6541 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 6601 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 6661 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 6721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 6781 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 6841 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 6901 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 6961 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 7021 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 7081 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 7141 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 7201 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 7261 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 7321 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 7381 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 7441 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 7501 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 7561 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 7621 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 7681 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 7741 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 7801 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 7861 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgcta //