LOCUS       Exported                6884 bp ds-DNA     circular SYN 09-DEC-2016
DEFINITION  Ligation of NEW_SEQUENCE_1/HindIII,XhoI into 
            pcDNA3.1(+)/HindIII,XhoI.
ACCESSION   .
VERSION     .
KEYWORDS    GS51405-1 pcDNA3.1(+)-NEW_SEQUENCE_1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     SECID/File created by SciEd Central, Scientific & Educational 
            Software
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             232..819
                     /gene="CMV"
                     /note="CMV"
     CDS             863..882
                     /gene="T7"
                     /note="T7"
     misc_feature    895..911
                     /gene="MCS''"
                     /note="MCS''"
     CDS             914..2441
                     /codon_start=1
                     /gene="'NEW_SEQUENCE_1'"
                     /note="Dopamine receptor"
                     /translation="LTTMKKSRGLSDYLWAWTLILSTLSGRSYGDTDRLAEIAANQEHS
                     IQLDQMPPDRTFHYPNIPSSQHDRRREGGKRKKFRKERLDEPCTAINETLRKNLLQELF
                     DDSYDKNNLPSANSTEVIVELTVQSITEISEFSSSFKADVWFSQIWRDPRLDFSDRNYC
                     LKNISLASHKLPTMWSPNVCFVNSKKVEIHASPSQNILLLIFPNGTVWLNFRVSLTGPC
                     KLDLTYFPMDRQSCNLVFESYSYNTAEVRIVWRDWDAVTIPDPDSKNLPDFELVNIEHM
                     NATLVYTAGLWDQLEVKFTFRRLYGYYVLQAYMPTYLSVFISWIAFWIDTKALPARITL
                     GVSSLMALTFQFGNIVKNLPRVSYVKALDIWMFGCVGFIFLSLVELAVVGFADKLEAKA
                     AAHNRCKEQLMMRSDSEQQWLSRLSGQRPQVSETNSDATHTIQINDGNGNVAAAKSEEK
                     RQKLLLQMESPMFVNGEKIDEISAKLFPLLFTAFNIFYWFYYIGMSGGFF"
     misc_feature    2442..2466
                     /gene="'MCS"
                     /note="'MCS"
     CDS             2754..3182
                     /gene="F1"
                     /note="F1"
     CDS             3187..3530
                     /gene="SV40"
                     /note="SV40"
     CDS             3592..4386
                     /codon_start=1
                     /gene="Neo"
                     /note="Neo"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5073..5743
                     /gene="pUC"
                     /note="pUC"
     CDS             complement(5888..6883)
                     /gene="Amp"
                     /note="Amp"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca ccatgaagaa gagcagagga ctcagtgatt acctgtgggc
      961 atggactctg atcctgagta ccctgtctgg aagatcttac ggagacactg acagactggc
     1021 cgaaatagcc gcaaaccagg agcacagtat tcagctcgac caaatgcctc cagacaggac
     1081 attccattat cctaacatac ctagcagcca gcatgaccgc agaagagaag gcggtaaacg
     1141 gaagaagttc agaaaggaaa gactggacga gccctgcacc gccatcaacg agactctcag
     1201 aaagaatctc ctccaagagc tgttcgacga ttcttacgac aagaataacc tcccaagcgc
     1261 caacagtaca gaggttatcg tcgaactcac cgtgcaatct attacagaga tctccgaatt
     1321 ctcatctagc ttcaaggcag acgtgtggtt tagccagatt tggcgcgacc ccaggctgga
     1381 cttctcagac agaaactatt gcctgaagaa cattagtctg gcaagccaca aactccccac
     1441 aatgtggagt cccaatgttt gctttgtcaa cagcaagaag gtggagatac acgcatcccc
     1501 atctcagaat atcctgctgc tgatattccc taacggaact gtctggctga atttccgggt
     1561 gagcctcact ggaccttgca aactggacct gacatatttc ccaatggatc ggcagtcttg
     1621 taatctggtc ttcgaatcat attcatataa cactgccgag gtcaggatcg tctggcggga
     1681 ctgggacgct gtgaccatac ctgatccaga ttcaaagaac ctgccagatt ttgagctcgt
     1741 gaatatcgag cacatgaatg ccaccctggt gtacactgca ggcctctggg accaactcga
     1801 agtgaagttt accttcagga gactctatgg ctactacgtt ctgcaagcct acatgccaac
     1861 ctacctcagt gtctttatca gctggatcgc attttggatc gacacaaaag cactgcccgc
     1921 aagaatcact ctgggcgtct cctccctgat ggcactgaca ttccagttcg gaaacattgt
     1981 taagaatctg cccagagtta gttatgtcaa ggccctcgac atttggatgt tcggctgtgt
     2041 gggcttcatc ttcctctctc tcgtggaact ggctgtcgtt ggcttcgcag acaagctgga
     2101 ggcaaaggca gctgcccaca acaggtgtaa agagcaactg atgatgcggt cagactccga
     2161 gcagcaatgg ctctctcgcc tcagtggcca gcggcctcag gtgtcagaaa ctaattctga
     2221 cgctacccat acaattcaaa ttaacgatgg aaacggaaac gttgcagctg ccaaatccga
     2281 ggaaaagcgc cagaaactgc tgctgcagat ggaatctcca atgttcgtga atggagaaaa
     2341 gatcgatgag atctccgcca aactgttccc tctcctgttc accgccttta acatcttcta
     2401 ttggttctat tacatcggca tgtctggcgg tttcttttga ctcgagtcta gagggcccgt
     2461 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2521 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2581 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2641 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     2701 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     2761 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2821 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2881 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2941 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3001 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3061 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3121 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3181 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     3481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     3541 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     3601 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3661 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3721 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3781 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3841 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3901 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3961 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4021 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4081 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     4141 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4201 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4261 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4321 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4381 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4441 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4501 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     4561 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4621 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4681 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4741 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4801 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4861 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4921 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5641 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     5701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5761 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5821 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5881 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5941 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6001 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6061 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6121 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6181 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6241 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6301 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6361 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6421 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6481 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6541 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6601 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6661 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6721 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6841 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
