LOCUS       pKIR1_0                17955 bp ds-DNA     circular     09-DEC-2016
DEFINITION  Cloning vector pKIR1_0 DNA, complete sequence.
ACCESSION   LC189563
VERSION     LC189563.1
KEYWORDS    .
SOURCE      Cloning vector pKIR1_0
  ORGANISM  Cloning vector pKIR1_0 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2016) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroki Tsutsui Nagoya University, Graduate School of
            Science; 1 Furo-cho, Nagoya, Aichi 464-8602, Japan
REFERENCE   2
  AUTHORS   Tsutsui,H. and Higashiyama,T.
  TITLE     pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene
            knockout in Arabidopsis thaliana
  JOURNAL   Plant Cell Physiol. (2016) In press
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     regulatory      76..1739
                     /note="AtRPS5A promoter"
                     /regulatory_class="promoter"
                     /label=AtRPS5A promoter
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=#66ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1742..1810
                     /note="3xFLAG"
                     /label=3xFLAG
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1811..1861
                     /note="NLS"
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1862..5962
                     /note="human-codon-optimized SpCas9"
                     /label=human-codon-optimized SpCas9
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5963..6010
                     /note="nucleoplasmin NLS peptide"
                     /label=nucleoplasmin NLS peptide
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6011..6013
                     /note="stop codon"
                     /label=stop codon
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      6023..6272
                     /note="Heat_shock_protein18.2 terminator"
                     /regulatory_class="terminator"
                     /label=Heat_shock_protein18.2 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6318..9398
                     /note="OLE1-TagRFP cassette"
                     /label=OLE1-TagRFP cassette
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9502..9526
                     /note="Right Border"
                     /label=Right Border
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    16037..16062
                     /note="Left Border"
                     /label=Left Border
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cgacgtcgca tgctcccggc cgccatggcc gcgggatatc acaagtttgt acaaaaaagc
       61 aggctccgcg gccgcgatcc ctcaactttt gattcgctat ttgcagtgca cctgtggcgt
      121 tcatcacatc ttttgtgaca ctgtttgcac tggtcattgc tattacaaag gaccttcctg
      181 atgttgaagg agatcgaaag taagtaactg cacgcataac cattttcttt ccgctctttg
      241 gctcaatcca tttgacagtc aaagacaatg tttaaccagc tccgtttgat atattgtctt
      301 tatgtgtttg ttcaagcatg tttagttaat catgcctttg attgatcttg aataggttcc
      361 aaatatcaac cctggcaaca aaacttggag tgagaaacat tgcattcctc ggttctggac
      421 ttctgctagt aaattatgtt tcagccatat cactagcttt ctacatgcct caggtgaatt
      481 catctatttc cgtcttaact atttcggtta attaaagcac gaacaccatt actgcatgta
      541 gaagcttgat aaactatcgc caccaattta tttttgttgc gatattgtta ctttcctcag
      601 tatgcagctt tgaaaagacc aaccctctta tcctttaaca atgaacaggt ttttagaggt
      661 agcttgatga ttcctgcaca tgtgatcttg gcttcaggct taattttcca ggtaaagcat
      721 tatgagatac tcttatatct cttacatact tttgagataa tgcacaagaa cttcataact
      781 atatgcttta gtttctgcat ttgacactgc caaattcatt aatctctaat atctttgttg
      841 ttgatctttg gtagacatgg gtactagaaa aagcaaacta caccaaggta aaatactttt
      901 gtacaaacat aaactcgtta tcacggaaca tcaatggagt gtatatctaa cggagtgtag
      961 aaacatttga ttattgcagg aagctatctc aggatattat cggtttatat ggaatctctt
     1021 ctacgcagag tatctgttat tccccttcct ctagctttca atttcatggt gaggatatgc
     1081 agttttcttt gtatatcatt cttcttcttc tttgtagctt ggagtcaaaa tcggttcctt
     1141 catgtacata catcaaggat atgtccttct gaatttttat atcttgcaat aaaaatgctt
     1201 gtaccaattg aaacaccagc tttttgagtt ctatgatcac tgacttggtt ctaaccaaaa
     1261 aaaaaaaatg tttaatttac atatctaaaa gtaggtttag ggaaacctaa acagtaaaat
     1321 atttgtatat tattcgaatt tcactcatca taaaaactta aattgcacca taaaattttg
     1381 ttttactatt aatgatgtaa tttgtgtaac ttaagataaa aataatattc cgtaagttaa
     1441 ccggctaaaa ccacgtataa accagggaac ctgttaaacc ggttctttac tggataaaga
     1501 aatgaaagcc catgtagaca gctccattag agcccaaacc ctaaatttct catctatata
     1561 aaaggagtga cattagggtt tttgttcgtc ctcttaaagc ttctcgtttt ctctgccgtc
     1621 tctctcattc gcgcgacgca aacgatcttc aggtgatctt ctttctccaa atcctctctc
     1681 ataactctga tttcgtactt gtgtatttga gctcacgctc tgtttctctc accacagccc
     1741 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
     1801 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
     1861 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
     1921 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     1981 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     2041 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     2101 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     2161 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     2221 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     2281 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     2341 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     2401 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     2461 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     2521 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     2581 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     2641 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     2701 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     2761 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     2821 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     2881 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     2941 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     3001 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     3061 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     3121 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     3181 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     3241 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     3301 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     3361 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     3421 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     3481 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     3541 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     3601 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     3661 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     3721 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     3781 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     3841 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     3901 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     3961 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     4021 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     4081 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     4141 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     4201 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     4261 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     4321 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     4381 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     4441 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     4501 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     4561 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     4621 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     4681 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     4741 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     4801 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     4861 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     4921 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     4981 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     5041 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     5101 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     5161 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     5221 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     5281 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     5341 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     5401 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     5461 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     5521 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     5581 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     5641 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     5701 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     5761 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     5821 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     5881 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     5941 cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     6001 aaagaaaaag taaaagggtg ggatatgaag atgaagatga aatatttggt gtgtcaaata
     6061 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     6121 ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat
     6181 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     6241 ggtatggact atggaatatg attaaagata aggcgcgccg acccagcttt cttgtacaaa
     6301 gtggtgatat cactagtgta tgtaggtata gtaacatgaa caagaacaga ctcaagtaca
     6361 agatcgcata cgaaaatgga aatggcaatg tcacttccac ataatcaaac acgaatcctc
     6421 atatcaacaa ggcctgagat tctaactagc tcataacaac ttagccaata gttacttgag
     6481 actaccaaat gtatgtagaa ctaaagacta agggacagag agttcgtcta aacaggtgaa
     6541 tctagtcgtt gttatctaat aaacaattca gccccaaatg cagaacacac atagagctct
     6601 ctattgattc aaattacgat ctgatactga taacgtctag atttttaggg ttaaagcaat
     6661 caatcacctg acgattcaag gtggttggat catgacgatt ccagaaaaca tcaagcaagc
     6721 tctcaaagct acactctttg ggatcatact gaactctaac aacctcgtta tgtcccgtag
     6781 tgccagtaca gacatcctcg taactcggat tgtgcacgat gccatggcta tacccaacct
     6841 cggtcttggt cacaccagga actctctggt aagctagctc cactccccag aaacaaccgg
     6901 cgccaaattg cgcgaattgc tgacctgaag acggaacatc atcgtcgggt ccttgggcga
     6961 ttgcggcgga agatgggtca gcttgggctt gaggacgaga cccgaatccg agtctgttga
     7021 aaaggttgtt cattggggat ttgtatacgg agattggtcg tcgagaggtt tgagggaaag
     7081 gacaaatggg tttggctctg gagaaagaga gtgcggcttt agagagagaa ttgagaggtt
     7141 tagagagaga tgcggcggcg atgagcggag gagagacgac gaggacctgc attatcaaag
     7201 cagtgacgtg gtgaaatttg gaacttttaa gaggcagata gatttattat ttgtatccat
     7261 tttcttcatt gttctagaat gtcgcggaac aaattttaaa actaaatcct aaatttttct
     7321 aattttgttg ccaatagtgg atatgtgggc cgtatagaag gaatctattg aaggcccaaa
     7381 cccatactga cgagcccaaa ggttcgtttt gcgttttatg tttcggttcg atgccaacgc
     7441 cacattctga gctaggcaaa aaacaaacgt gtctttgaat agactcctct cgttaacaca
     7501 tgcagcggct gcatggtgac gccattaaca cgtggcctac aattgcatga tgtctccatt
     7561 gacacgtgac ttctcgtctc ctttcttaat atatctaaca aacactccta cctcttccaa
     7621 aatatataca catctttttg atcaatctct cattcaaaat ctcattctct ctagtaaaca
     7681 agaacaaaaa aatggcggat acagctagag gaacccatca cgatatcatc ggcagagacc
     7741 agtacccgat gatgggccga gaccgagacc agtaccagat gtccggacga ggatctgact
     7801 actccaagtc taggcagatt gctaaagctg caactgctgt cacagctggt ggttccctcc
     7861 ttgttctctc cagccttacc cttgttggaa ctgtcatagc tttgactgtt gcaacacctc
     7921 tgctcgttat cttcagccca atccttgtcc cggctctcat cacagttgca ctcctcatca
     7981 ccggttttct ttcctctgga gggtttggca ttgccgctat aaccgttttc tcttggattt
     8041 acaagtaagc acacatttat catcttactt cataattttg tgcaatatgt gcatgcatgt
     8101 gttgagccag tagctttgga tcaatttttt tggtcgaata acaaatgtaa caataagaaa
     8161 ttgcaaattc tagggaacat ttggttaact aaatacgaaa tttgacctag ctagcttgaa
     8221 tgtgtctgtg tatatcatct atataggtaa aatgcttggt atgataccta ttgattgtga
     8281 ataggtacgc aacgggagag cacccacagg gatcagacaa gttggacagt gcaaggatga
     8341 agttgggaag caaagctcag gatctgaaag acagagctca gtactacgga cagcaacata
     8401 ctggtgggga acatgaccgt gaccgtactc gtggtggcca gcacactact atgagcgagc
     8461 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     8521 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     8581 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     8641 acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     8701 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     8761 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     8821 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca
     8881 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     8941 tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg
     9001 ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg
     9061 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     9121 tccctagcaa actggggcac aagtgaatcg ttcaaacatt tggcaataaa gtttcttaag
     9181 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     9241 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     9301 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     9361 taaattatcg cgcgcggtgt catctatgtt actagatcac tagtgcggcc gcctgcaggt
     9421 cgaccatatg ggagagctca agcttagctt gagcttggat cagattgtcg tttcccgcct
     9481 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     9541 cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     9601 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     9661 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     9721 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     9781 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     9841 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     9901 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     9961 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
    10021 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
    10081 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
    10141 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
    10201 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
    10261 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
    10321 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
    10381 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
    10441 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
    10501 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
    10561 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
    10621 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
    10681 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
    10741 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
    10801 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
    10861 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
    10921 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
    10981 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
    11041 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
    11101 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
    11161 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
    11221 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
    11281 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
    11341 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
    11401 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
    11461 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
    11521 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
    11581 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
    11641 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
    11701 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
    11761 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
    11821 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
    11881 ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
    11941 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
    12001 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
    12061 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
    12121 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
    12181 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
    12241 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
    12301 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
    12361 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
    12421 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
    12481 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
    12541 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
    12601 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
    12661 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
    12721 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
    12781 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    12841 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    12901 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    12961 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
    13021 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    13081 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    13141 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    13201 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
    13261 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    13321 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    13381 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    13441 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    13501 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    13561 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    13621 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    13681 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    13741 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    13801 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    13861 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    13921 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    13981 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    14041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    14101 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    14161 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    14221 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    14281 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    14341 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    14401 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    14461 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    14521 aaaactcacg ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt
    14581 attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat
    14641 tatgtgctta gtgcatctaa tcgcttgagt taacgccggc gaagcggcgt cggcttgaac
    14701 gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
    14761 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
    14821 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
    14881 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
    14941 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
    15001 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
    15061 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
    15121 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
    15181 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
    15241 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
    15301 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
    15361 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
    15421 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
    15481 gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg gcgactgccc
    15541 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
    15601 tgctgcttgg atgcccgagg catagactgt accccaaaaa aacatgtcat aacaagaagc
    15661 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
    15721 gcgtgacggc agttacgcta cttgcattac agcttacgaa ccgaacgagg cttatgtcca
    15781 ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    15841 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    15901 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    15961 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    16021 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    16081 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaattg aattatcagc
    16141 ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
    16201 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
    16261 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
    16321 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
    16381 ctttattgcc aaatgtttga acgatctgct tgactctagg gaattaattc ctgaatcact
    16441 gcgaccggcc ctcccgcgac ccagccgagc gagcttagcg aactgtggac gagaactgtg
    16501 ccaccaagcg taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc
    16561 tgaggcgttc tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg
    16621 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
    16681 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
    16741 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
    16801 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagcctt ggcgatcctg
    16861 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
    16921 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
    16981 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaatccgc
    17041 gtgcacgagt gccggacttc ggggcagtcc tcgcccaaag catcagctca tcgagagcct
    17101 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
    17161 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
    17221 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatagcatcc atagcctccg
    17281 cgaccggctg aagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
    17341 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ctccccaatg tcaagcactt
    17401 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
    17461 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
    17521 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
    17581 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcataggg gggattcgag
    17641 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
    17701 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
    17761 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctcagtg
    17821 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
    17881 ggcggggtca taacgtgact cccttaattc tcatgtatga taattcgagg gtacccgggg
    17941 atcctctaga gggcc    
//
