LOCUS       pGL3-ank3_1-luc         6696 bp    DNA     circular UNA 12-DEC-2016
DEFINITION  Concatenation of 2 sequences - NheI-KpnI digestion - ligation.
ACCESSION   urn:local:.:1363016736586.137
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    640
                     /created_by="User"
                     /modified_by="User"
     misc_feature    1..>5
                     /note="multiple cloning site"
     misc_feature    <1900..1936
                     /note="multiple cloning site"
     misc_feature    complement(427..434)
                     /created_by="User"
                     /modified_by="User"
     rep_origin      4196
                     /note="ColE1-derived plasmid replication origin"
     source          1..>5
                     /organism="Cloning vector pGL3-Basic"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45857"
                     /note="luciferase reporter vector"
     source          <1900..6696
                     /organism="Cloning vector pGL3-Basic"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45857"
                     /note="luciferase reporter vector"
     rep_origin      5950..6405
                     /note="f1 origin"
     misc_feature    1781..1792
                     /created_by="User"
     misc_feature    1336..1345
                     /created_by="User"
     misc_feature    19..1818
                     /Original_Bases="MUSMUSCUUSCGCCATCAATGCTCCCTGCCAGCCTTTGAGT
                     GGTGGTTTACTGTTTGTAATGCGCAGCCGCAGAACAACCATTAAATAACCCACAGTGC
                     GCAGGATCTCCATGGGTCGCTGGAGCAAGCAMUSMUSCUUSCTTGTAACCCTGGTGTC
                     CATGACAGAGCTGTCTCAAGCTGCCCTCATGCTCACTCATTTTTAGGGGGGTGGCGCT
                     TGGGGAGTCCAGATTTGTTGAACATAGGTACAACCCTGCTTAAATMUSMUSCUUSGAT
                     AGATGAGAAATGGTGATTTTGGCTTCAGTATTTTAGCATTGTGTCTCCAGTATTAAAT
                     AGCTCCATGTACCTCACTGAAAACTGGCCTTCTGGGTCTCATTTACGCTCCTGTATGT
                     CMUSMUSCUUSTTTGATCATATTAATTAAAATCCACTTCCCTGAGCCAGCCTCCTCTC
                     TGGATTCCCGCTCGTTCCTCCCTTCCTAGTGTACATGTGTTTTAGCTCAAGGGACCTG
                     CTTCCATCTTAAAGGMUSMUSCUUSTGATCTGGCCGGGATTGGGGGGGGGGGACCCAG
                     AACCTTATTCCTTCCTAACAAGAATGGATTCCTAACACTTTCCAAAGATGCTTAAGCG
                     ACCACACCCGACAGTAAATCAGCTGTGTTMUSMUSCUUSCCGATGCGTGGACCATTTT
                     GGAGACTTTCCACAAAGGAGTGAAGTCACCCAGAGACCTCTCAAAATCTTGGTGGCCC
                     AGAGCAGGAGTGTGTGCCTCTGTGTGCTTGGGGACCCTGTGGTMUSMUSCUUSGGCTG
                     TAATGTGGCCAGAGACCTGCACTGACCTGAGTGCCTTATCCAGGAGCACAGGATGTTT
                     CTGGCACAGAAAAAGCCAGGGGCTGAACCTTTTGGATTTCTTGAATATTCTGGAGGGM
                     USMUSCUUSCTCCTTTTCATGGTTGGTTTGATATATTCATGACCAAAGCTTGAATGTG
                     GTACTGGCAACTTAGGTATGGGCTCCGTGTGACAGATTCTTCATTAGAAGTCCATAGC
                     TTTGGTTAGGCAAMUSMUSCUUSTCTGCAAACTTTATTTTATTTTATTTTATTTATTT
                     TTTGCTAATTACATTTTAAAACAAAACAAACAACAACAACAAAAACCACCTCACTGGG
                     TAAGAATCCAGCTTTGTCTTCTGTAGTMUSMUSCUUSAACAGGTCTCTTTATTGTTTT
                     TAATCATGGGGTTGGGGTTGGGGGTGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
                     AGAATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTMUSMUSCUUSTCACGAA
                     CCAGTAACCCGATCTCTCGTTACCACCCCCACTGCTGCCTCCTGGGTATACCCAATGC
                     TTTCAACACATGTGTCCTCTGGAGGCTTGATCTCAGAGCATTTACATTCGGCTTGMUS
                     MUSCUUSGCTTCCCAGCTTGCAAACTGCAGTGAGCCAGCCCCTGGGACGCTTCAGAGA
                     TTCGTCAGCAGTCTAGCTTATTCCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
                     TGTGTGTGTGTMUSMUSCUUSGTGCAGCGCACATGCACGTATGCCTCCCCCTTTCTCT
                     CTCTCACCATTTCCAAGCTAGAATCACCGTGGAGAAGCAAGCCCTGAAATGTTGTCAA
                     GATCTTTTCTCGGGGTAACGACTGGMUSMUSCUUSCTAAAAAAACAAATAAAAAGAGA
                     GAGAAAGGAAGAAAAAGAAAGAAGGAAGAAAGGAAGGAAGAAAACAAAGGGTTGGAAT
                     ATTGATTTTTCTTTGGAGGAATAACCAGGGCTTTGCTGGMUSMUSCUUSAGAGAACAC
                     CCAGTTCCTCCCTGAGGCGAGCTGAGTTGCCCGTGACAGCAGCCAGGGCTGGCTTGAA
                     GCAGCCGCTCTGCCTGGGGAGTTCCCTAAATGGACTTTGGTCTCAATGCTGTGMUSMU
                     SCUUS"
                     /note="Geneious type: Editing History Replacement"
     CDS             1966..3618
                     /gene="luc+"
                     /codon_start=1
                     /transl_table=1
                     /product="luciferase"
                     /protein_id="AAA89082.1"
                     /db_xref="GI:1200460"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3939..3958)
                     /note="RV primer4 sequencing primer binding site"
     misc_feature    1149..1219
                     /created_by="User"
     misc_feature    6..1899
                     /note="Geneious type: Concatenated sequence"
     misc_feature    638..648
                     /created_by="User"
     misc_feature    1904..1
                     /note="Geneious type: Concatenated sequence"
     CDS             complement(4958..5818)
                     /gene="AmpR"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /transl_table=1
                     /product="beta-lactamase"
                     /protein_id="AAA89083.1"
                     /db_xref="GI:1200461"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     polyA_signal    6536..6689
                     /note="upstream poly(A) signal"
     misc_feature    426..435
                     /created_by="User"
     misc_feature    6
                     /Original_Bases
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1899
                     /Original_Bases
                     /note="Geneious type: Editing History Insertion"
     gene            complement(4958..5818)
                     /gene="AmpR"
     gene            1966..3618
                     /gene="luc+"
     polyA_signal    3650..3871
                     /note="SV40 late poly(A) signal"
     primer_bind     6638..6657
                     /note="RV primer3 sequencing primer binding site"
     misc_feature    1783..1792
                     /created_by="User"
     primer_bind     complement(1967..1989)
                     /note="GL primer2 sequencing primer binding site"
     ligation        2..5
     ligation        1900..1903
     misc_feature    1782..1791
                     /created_by="User"
                     /modified_by="User"
     misc_feature    425..434
                     /created_by="User"
     misc_feature    1780..1791
                     /created_by="User"
                     /modified_by="User"
ORIGIN      
        1 ggtaccgggt ggggcaaccg ccatcaatgc tccctgccag cctttgagtg gtggtttact
       61 gtttgtaatg cgcagccgca gaacaaccat taaataaccc acagtgcgca ggatctccat
      121 gggtcgctgg agcaagcact tgtaaccctg gtgtccatga cagagctgtc tcaagctgcc
      181 ctcatgctca ctcattttta ggggggtggc gcttggggag tccagatttg ttgaacatag
      241 gtacaaccct gcttaaatga tagatgagaa atggtgattt tggcttcagt attttagcat
      301 tgtgtctcca gtattaaata gctccatgta cctcactgaa aactggcctt ctgggtctca
      361 tttacgctcc tgtatgtctt tgatcatatt aattaaaatc cacttccctg agccagcctc
      421 ctctctggat tcccgctcgt tcctcccttc ctagtgtaca tgtgttttag ctcaagggac
      481 ctgcttccat cttaaaggtg atctggccgg gattgggggg gggggaccca gaaccttatt
      541 ccttcctaac aagaatggat tcctaacact ttccaaagat gcttaagcga ccacacccga
      601 cagtaaatca gctgtgttcc gatgcgtgga ccattttgga gactttccac aaaggagtga
      661 agtcacccag agacctctca aaatcttggt ggcccagagc aggagtgtgt gcctctgtgt
      721 gcttggggac cctgtggtgg ctgtaatgtg gccagagacc tgcactgacc tgagtgcctt
      781 atccaggagc acaggatgtt tctggcacag aaaaagccag gggctgaacc ttttggattt
      841 cttgaatatt ctggagggct ccttttcatg gttggtttga tatattcatg accaaagctt
      901 gaatgtggta ctggcaactt aggtatgggc tccgtgtgac agattcttca ttagaagtcc
      961 atagctttgg ttaggcaatc tgcaaacttt attttatttt attttattta ttttttgcta
     1021 attacatttt aaaacaaaac aaacaacaac aacaaaaacc acctcactgg gtaagaatcc
     1081 agctttgtct tctgtagtaa caggtctctt tattgttttt aatcatgggg ttggggttgg
     1141 gggtggggga gagagagaga gagagagaga gagagagaga atgtgtgtgt gtgtgtgtgt
     1201 gtgtgtgtgt gtgtgttttc acgaaccagt aacccgatct ctcgttacca cccccactgc
     1261 tgcctcctgg gtatacccaa tgctttcaac acatgtgtcc tctggaggct tgatctcaga
     1321 gcatttacat tcggcttggc ttcccagctt gcaaactgca gtgagccagc ccctgggacg
     1381 cttcagagat tcgtcagcag tctagcttat tcctgtgtgt gtgtgtgtgt gtgtgtgtgt
     1441 gtgtgtgtgt gtgtgtgtgt gcagcgcaca tgcacgtatg cctccccctt tctctctctc
     1501 accatttcca agctagaatc accgtggaga agcaagccct gaaatgttgt caagatcttt
     1561 tctcggggta acgactggct aaaaaaacaa ataaaaagag agagaaagga agaaaaagaa
     1621 agaaggaaga aaggaaggaa gaaaacaaag ggttggaata ttgatttttc tttggaggaa
     1681 taaccagggc tttgctggag agaacaccca gttcctccct gaggcgagct gagttgcccg
     1741 tgacagcagc cagggctggc ttgaagcagc cgctctgcct ggggagttcc ctaaatggac
     1801 tttggtctca atgctgtgac tctttgccgt gggtttggat gtgggaacac ggaaggtgat
     1861 ctcctgcctc gtctcaactc ccctgatctc aaggaaaggc tagcccgggc tcgagatctg
     1921 cgatctaagt aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa
     1981 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
     2041 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
     2101 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
     2161 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
     2221 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
     2281 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
     2341 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
     2401 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
     2461 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
     2521 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
     2581 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
     2641 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
     2701 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     2761 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg
     2821 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     2881 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     2941 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     3001 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     3061 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     3121 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     3181 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     3241 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     3301 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     3361 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     3421 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     3481 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     3541 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     3601 ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     3661 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3721 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3781 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3841 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     3901 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     3961 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     4021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4081 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4141 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4381 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4561 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4621 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4801 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4861 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4921 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4981 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5041 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5101 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5161 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5221 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5281 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5341 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5401 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5461 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5521 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5581 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5641 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5701 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5761 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5821 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5941 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6001 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6061 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6121 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6181 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6241 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6301 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6361 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca
     6421 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
     6481 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
     6541 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     6601 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     6661 tgcaagtgca ggtgccagaa catttctcta tcgata
//
